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U-PLEX Obesity Combo 1 (human)

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U-PLEX Combos offer popular panels of biomarkers that have been grouped together for convenient ordering. They have been tested for multiplex compatibility and are fulfilled as a kit. The U-PLEX Obesity Combo 1 (human) contains assays for five analytes (Table 1). Ghrelin, Glucagon and Leptin are traditional metabolic markers; BDNF and FGF-21 are emerging biomarkers that have been associated with metabolic syndrome and obesity. The assays can be used for quantitative measurements in the sample types listed in the "Specifications" tab below, and offer detection limits (LLODs) in the sub- to low pg/mL range.

Table 1. List of analytes and typical detection limits

Analyte	LLOD - ULOD	units
BDNF	0.72 - 2,000	pg/mL
FGF-21	2.8 - 8,230	pg/mL
Ghrelin (total)	1.7 - 2,710	pg/mL
Glucagon	0.13 - 156	pM
Leptin	14 - 47,500	pg/mL

Specifications
Kit Contents
Documentation
References

Application(s)		Metabolic
Analyte(s)		BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO SECTOR S 600MM , SECTOR Imager 6000 , MESO QuickPlex SQ 120MM , MESO QuickPlex SQ 120 , MESO SECTOR S 600
U-PLEX Group Compatibility		Metabolic Group 1
Plate Type		96-well
Sample Type		Serum, EDTA Plasma, P800 Plasma
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component
Schedule B Code		3822.19.0000

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 5-Assay (300 µL)	2-8 °C	1 each (1 vial)

U-PLEX FGF-21 Antibody Set	2-8 °C	1 each (1 plate size)

U-PLEX Ghrelin (total) Antibody Set	2-8 °C	1 each (1 plate size)

U-PLEX Glucagon Antibody Set	2-8 °C	1 each (1 plate size)

U-PLEX Human Leptin Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX BDNF Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

Calibrator 13	2-8 °C	1 vial (1 vial)

Calibrator 14	2-8 °C	1 vial (1 vial)

U-PLEX 5-Assay, 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)

Diluent 11	≤-10 °C	1 bottle (10 mL)

Diluent 13	≤-10 °C	1 bottle (10 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

Aprotinin	2-8 °C	1 vial (50 μL)

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 5-Assay (1.8 mL)	2-8 °C	1 each (1 vial)

U-PLEX FGF-21 Antibody Set	2-8 °C	1 each (5 plate size)

U-PLEX Ghrelin (total) Antibody Set	2-8 °C	1 each (5 plate size)

U-PLEX Glucagon Antibody Set	2-8 °C	1 each (5 plate size)

U-PLEX Human Leptin Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX BDNF Antibody Set (1 Plate Size)	2-8 °C	1 each (5 plate size)

Calibrator 13	2-8 °C	5 vial (1 vial)

Calibrator 14	2-8 °C	5 vial (1 vial)

U-PLEX 5-Assay, 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)

Diluent 11	≤-10 °C	1 bottle (50 mL)

Diluent 13	≤-10 °C	1 bottle (50 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

Aprotinin	2-8 °C	1 vial (250 μL)

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 5-Assay (1.8 mL)	2-8 °C	5 each (1 vial)

U-PLEX FGF-21 Antibody Set	2-8 °C	5 each (5 plate size)

U-PLEX Ghrelin (total) Antibody Set	2-8 °C	5 each (5 plate size)

U-PLEX Glucagon Antibody Set	2-8 °C	5 each (5 plate size)

U-PLEX Human Leptin Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX BDNF Antibody Set (1 Plate Size)	2-8 °C	5 each (5 plate size)

Calibrator 13	2-8 °C	25 vial (1 vial)

Calibrator 14	2-8 °C	25 vial (1 vial)

U-PLEX 5-Assay, 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

U-PLEX Stop Solution	2-8 °C	5 bottle (40 mL)

Diluent 11	≤-10 °C	5 bottle (50 mL)

Diluent 13	≤-10 °C	5 bottle (50 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

Aprotinin	2-8 °C	5 vial (250 μL)

Order Details

Catalog No

Size

Price in USD

Qty

K15277K-1

1 plate

$1,788.00

Usually ships in 1-3 days

K15277K-2

5 plates

$7,506.00

Usually ships in 1-3 days

K15277K-4

25 plates

$33,040.00

Usually ships in 1-3 days

Contact Information

Meso Scale Japan 株式会社
電話: 03.6265.7384
〒107-0062
東京都港区南青山 1-1-1 新青山ビル西館１４階 Vistra　Japan内

Product Inserts		U-PLEX Metabolic Group 1 (human) Multiplex Insert
Datasheets		U-PLEX-Human-BDNF.pdf U-PLEX-Human-FGF-21.pdf U-PLEX-Human-Ghrelin-total.pdf U-PLEX-Human-Glucagon.pdf U-PLEX-Human-Leptin.pdf
Product Highlights		U-PLEX Platform Highlights
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 10, Diluent 11, Diluent 12, Diluent 13, Diluent 15, Diluent 16 and Diluent 17 MSD Calibrators and Controls MSD GOLD Read Buffer B U-PLEX Linker Set

Title	Journal	Year
Leptin levels are associated with multiple sclerosis risk Biström M, Hultdin J, Andersen O, Alonso-Magdalena L, Jons D, Gunnarsson M, Vrethem M, Sundström P	Multiple Sclerosis	2020
Genetic Deletion and Pharmacological Inhibition of Phosphodiesterase 10A Protects Mice From Diet-Induced Obesity and Insulin Resistance. Nawrocki AR, Rodriguez CG, Toolan DM, Price O, Henry M, Forrest G, Szeto D, Keohane CA, Pan Y, Smith KM, Raheem IT, Cox CD, Hwa J, Renger JJ, Smith SM.	Diabetes	2014
A multi-biomarker score measures rheumatoid arthritis disease activity in the BeSt study. Hirata S, Dirven L, Shen Y, Centola M, Cavet G, Lems WF, Tanaka Y, Huizinga TW, Allaart CF.	Rheumatology (Oxford)	2013
Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice. Vasu S, Moffett RC, McCluskey JT, Hamid MH, Irwin N, Flatt PR.	Gene Ther.	2013
Efficacy and safety of mavrilimumab in subjects with rheumatoid arthritis. Burmester GR, Weinblatt ME, McInnes IB, Porter D, Barbarash O, Vatutin M, Szombati I, Esfandiari E, Sleeman MA, Kane CD, Cavet G, Wang B, Godwood A, Magrini F; EARTH Study Group.	Ann Rheum Dis.	2013
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U-PLEX Obesity Combo 1 (human)

Kit Contents for Catalog No. K15277K-1

Kit Contents for Catalog No. K15277K-2

Kit Contents for Catalog No. K15277K-4

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