| FAQ | Category | |
V-PLEX and V-PLEX Plus Questions
Does the V stand for validated?
V-PLEX products are MSD's... | Assays | |
What amount (micrograms) of lysate should I use?
The performance of the assay will depend greatly on the abundance of the protein... | Assays | |
What are kit layout files?
The first step in data analysis is identifying the spot location of each assay. ... | Software | |
What are the dimensions of your 96- and 384-well plates?
Please see immunoassay plates for plate-specific information.... | Plates | |
What are the MSD Product Purchase Terms & Conditions?
When purchasing products or services from MSD, you are agreeing to our Term... | Ordering | |
What are the release specifications (QC) for the kits?
All of our validated kits come with a Certificate of Analysis (COA) that documen... | Assays | |
What browsers are supported by the MSD website?
To ensure you have the best experience possible, we recommend using the followin... | | |
What do I need to purchase along with the R-PLEX and/or U-PLEX Antibody Sets to perform a multiplex assay?
Multiplex assays, using either R-PLEX antibody sets or a combination of R-PLEX a... | Assays | |
What do I need to purchase along with the R-PLEX Antibody Set to perform a singleplex assay?
The R-PLEX Antibody Set includes a matched antibody set and calibrator. MSD GOLD... | Assays | |
What is the best way to thaw MSD reagents that have been shipped frozen?
For diluents (use at room temperature):
Option 1:
The day befor... | Reagents Diluents Buffers | |
What is the binding capacity of avidin and streptavidin plates?
For Standard plates, the avidin or streptavidin-coated electrode surface is capa... | | |
What is the difference between an R-PLEX Antibody Set and a U-PLEX Antibody Set?
R-PLEX Antibody Sets contain a biotinylated capture antibody, a SULFO-TAG conjug... | Assays | |
What is the difference between anti-hu IL-8 and IL-8 (HA) detection antibodies?
The anti-hu IL-8 (HA) antibody has been validated and is recommended when high I... | Assays | |
What is the difference between V-PLEX and V-PLEX Plus kits?
The V-PLEX Plus kits include additional items (controls, wash buffer, and plate ... | Assays | |
What is the MSD Lysis Buffer formulation?
150 mM NaCl
20 mM Tris, pH 7.5
1 mM EDTA
1 mM EGTA
1% T... | Assays | |
What is the MSD wash buffer formulation?
The MSD wash buffer is a 20-fold concentrated phosphate buffered solution with s... | | |
What is the recommended reagent formulation for conjugation?
Carrier proteins (e.g. BSA and gelatin) and buffer additives such as glycerol, d... | Reagents Diluents Buffers | |
What is the sample volume required for the cytokine 30-plex or the 40-plex V-PLEX kits?
The sample volume required depends on sample type and dilution factor used. Reco... | Assays | |
What is the shelf life of the Human Aß42 and Human Total Tau kits?
Plates expire 15 months from the date of manufacture. Kit expiration dates are b... | Assays | |
What is the turnaround time for orders?
Catalog Items
Our catalog assays generally ship within 1-3 business days.
Cust... | Assays
Ordering | |
What other lysis buffers have been used with MSD assays?
Various modified RIPA buffers with low concentrations of ionic detergents and ur... | Assays
Reagents Diluents Buffers | |
What parameters can I optimize for my particular sample?
End users can vary the following parameters:
Concentration of detection a... | Assays | |
What plates do I purchase for R-PLEX multiplex assays?
R-PLEX multiplex assays use 10-spot 96-well U‑PLEX plates, available as U-PLEX D... | Assays | |
What should I do if I have less than 25 µL of sample?
A minimum of 25 µL of solution is required to prevent the plate surface fr... | Assays | |
What should I do if the read buffer is added to the plate, but the instrument is not available?
If the instrument is not available, MSD recommends that you remove the read buff... | Assays
Reagents Diluents Buffers | |