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Read buffer is an essential component of MSD immunoassays: it is required for the generation of electrochemiluminescence (ECL) signals. MSD GOLD Read Buffer B was optimized for use in all MSD assays and can be used as a replacement for MSD GOLD Read Buffer A and MSD Read Buffer T (4x). MSD GOLD Read Buffer B is also recommended for assays that measure whole cell, vesicles, or other membrane-containing analytes. MSD GOLD Read Buffer B has stringent quality control release criteria that ensure lot-to-lot reproducibility. The specifications are designed to meet the requirements of critical assays and longitudinal studies. MSD GOLD Read Buffer B is provided at the working concentration. We recommend using MSD GOLD Read Buffer B at the supplied concentration without any dilution.