Developing novel immunoassays using MSD’s platform is fast and easy, and transferring existing ELISAs can often be accomplished with minimal optimization in less than 2 days. 
Our MULTI-ARRAY technology provides inherently better sensitivity and a broader dynamic range than ELISAs. This means fewer samples fall below the assay detection range. 

Compared to ELISA technology, MSD assays:

• Require far less sample 
• Provide more sensitive and precise results
• Handle complex biological matrices easily
• Use simpler, faster protocols
• Quantify up to 10 analytes per well
• Measure elevated and normal expression samples without multiple dilutions

 

Unlike standard ELISA plate readers, MSD instruments provide increased throughput with fast read times (1–3 minutes per plate) and no downtime for cleaning or user maintenance.

	ELISA	MSD
Sample volume requirement	50–100 μL (per analyte)	10–25 μL (up to 10 analytes)
Multiplex panels	No	10 analytes simultaneously
Flexible panel configuration	No	Yes (U-PLEX)
Dynamic range	1–2 logs	3–4+ logs
Matrix effects	Yes	Greatly reduced
Simple protocols	No	Yes
Wash steps	Many	Typically 1–3
Read times	Slow	Up to 960 results in 1 to 3 min
Rapid throughput	No	Yes
Instrument maintenance	Daily cleaning and calibration	No user maintenance required

For more information on converting an existing ELISA to the MSD platform, contact an account manager. 

For more information on how our technology compares to others, download our technology brochure.