Can I increase my sample volume to improve the sensitivity of the assay?

For the sample incubation step, most MSD assays use between 25-50 μL/well sample volumes which is optimal for the suggested protocol (with 1-2 hour sample incubation).  Increasing sample volumes may yield higher signal, however, higher sample volumes may need longer incubation times to reach equilibrium.  If additional sensitivity is required, please consult your Application Scientist.  

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