How much secondary antibody do I use for my assay development?
For initial assay development, it is recommended that a 1:1 primary:secondary antibody ratio be used. For example, 1 µg/mL primary detection antibody:1 µg/mL secondary detection antibody. A recommended starting concentration for each antibody is 1 µg/mL (6.7 nM), and can be adjusted according to initial assay results.
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