Our new Phospho-Akt (Ser473) Kit (catalog# K150MND) and Total Akt Kit (catalog# K150MOD) provide many advantages for the absolute quantification of Akt1 in biological samples. The new assays have been developed with recombinant calibrators and highly characterized critical reagents for greater sensitivity and better reproducibility. The recombinant calibrator included in the kits can be used to generate a standard curve to quantitate phosphorylated Akt at serine 473 or total Akt levels in human, mouse, rat, and non-human primate cell lysates as well as tissue lysate samples. Read all of our Akt product FAQs for more details.