| FAQ | Category | |
Are the Aß and Tau calibrators in the validated Human Aß42 and Human Total Tau kits native protein?
The Total Tau assay is a recombinant Tau 441 protein expressed in E.coli. The A&... | Assays
Ordering | |
Can I conjugate proteins and antibodies with SULFO-TAG in my lab?
MSD provides SULFO-TAG NHS-Ester and you can follow the straightforward conjugat... | Assays | |
Can I denature the cell lysates?
Yes, ionic detergents or urea can be used to denature proteins; however, these r... | Assays | |
Can I develop my own phosphoprotein assays?
Yes, immobilizing antibodies on bare plates and labeling detection antibodies is... | Assays | |
Can I increase my sample volume to improve the sensitivity of the assay?
For the sample incubation step, most MSD assays use between 25-50 μL/well sam... | Assays | |
Can I order additional calibrator for the Phospho-Akt (Ser473) Kits and Total Akt Kits?
Can I order additional calibrator for the Phospho-Akt (Ser473) Kits and Total Ak... | Assays | |
Can I perform R-PLEX Singleplex assays on plates other than Small Spot Streptavidin plates?
R‑PLEX Singleplex assays can be developed on any MSD streptavidin- or avidin-coa... | Assays | |
Can I run my samples un-diluted to increase sensitivity?
For optimal performance, you should use the minimum sample dilution recommended ... | Assays | |
Can I run tissue homogenates or other sample types with V-PLEX kits?
MSD has not validated other sample types with the V-PLEX kits, but other sample ... | Assays | |
Can I use 384-well plates with R-PLEX?
You can use as little as 10 µL per well to coat a 384‑well plate. Calculat... | Assays | |
Can I use my own diluents and wash buffer when running R-PLEX assays?
Diluents used to generate the reported data are recommended as starting diluents... | Assays | |
Can I use my own diluents and wash buffer when running R-PLEX assays?
Diluents used to generate the reported data are recommended as starting diluents... | Assays | |
Can I use the same calibrator when testing sample types other than serum, plasma, or urine?
Yes, the same calibrator and thus the same calibration curve can be used for mul... | Assays | |
Can R-PLEX calibrators be purchased individually?
The R-PLEX calibrators are only available as part of an R-PLEX Antibody Set.... | Assays | |
Can sample dilutions other than the recommended dilutions be run? Can you lower the dilution factor to increase sensitivity?
Dilution linearity for CSF is provided in the product insert and data sheets. To... | Assays | |
Can the Aß42 assay be run in a homogenous format?
While the Aβ42 assay was validated with a washed assay, a homogenous protoc... | Assays | |
Can the assays be used with tissue lysates?
Yes, brain tissue homogenates, liver homogenates, homogenates from Matrigel plug... | Assays | |
Can the IL-8 HA assay be purchased as a singleplex?
No, the IL-8 HA is only available in select multiplex panels.... | Assays | |
Can the Validated Human Aß42 and Human Total Tau assays be multiplexed together or with the other Aß peptide assays?
The assays can be provided as a custom multiplex with comparable performance to ... | Assays | |
Can V-PLEX orders ship within the same day?
No. V-PLEX orders generally ship within 1-3 business days.... | Ordering
Assays | |
Can you measure Aß42 levels in plasma with the validated Human Aß42 Kit?
The assay was validated with human CSF. MSD is currently working on blood-based ... | Assays | |
Do I have to use the specific diluents provided on the R PLEX datasheet for my assay? What if I am multiplexing?
The Diluent Compatibility table on the R-PLEX datasheet lists the diluents that ... | Assays | |
Does MSD offer a multiplex for Aß42, Total Tau, and p-Tau (181)?
No, but the assays can be provided as a custom multiplex. However, the custom mu... | Assays | |
Does MSD offer the 12F4 antibody as a detection antibody with a 6E10 capture in the Human (6E10) Abeta 42 Ultra-Sensitive Kit?
No. We currently do not offer 12F4 as a detection antibody. We have not found an... | Assays | |
Does MSD use controls in the development of validated assays? If so, are they samples or diluted standards?
We test controls as part of the validation process for V‑PLEX assays. The t... | Assays | |