Ozone induces glucose intolerance and systemic metabolic effects in young and aged brown Norway rats.

Bass V, Gordon CJ, Jarema KA, Macphail RC, Cascio WE, Phillips PM, Ledbetter AD, Schladweiler MC, Andrews D, Miller D, Doerfler DL, Kodavanti UP.
Journal   Toxicol Appl Pharmacol.
Species  
Analytes Measured   A2M , Adiponectin , AGP , Glucagon , IL-6 , Insulin , Leptin
Matrix Tested   Serum
Year   2013
Volume   273
Page Numbers   551-560
Application   Cytokines and Chemokines , Metabolic , Toxicology
Abstract
Air pollutants have been associated with increased diabetes in humans. We hypothesized that ozone would impair glucose homeostasis by altering insulin signaling and/or endoplasmic reticular (ER) stress in young and aged rats. One, 4, 12, and 24month old Brown Norway (BN) rats were exposed to air or ozone, 0.25 or 1.0ppm, 6h/day for 2days (acute) or 2d/week for 13weeks (subchronic). Additionally, 4month old rats were exposed to air or 1.0ppm ozone, 6h/day for 1 or 2days (time-course). Glucose tolerance tests (GTT) were performed immediately after exposure. Serum and tissue biomarkers were analyzed 18h after final ozone for acute and subchronic studies, and immediately after each day of exposure in the time-course study. Age-related glucose intolerance and increases in metabolic biomarkers were apparent at baseline. Acute ozone caused hyperglycemia and glucose intolerance in rats of all ages. Ozone-induced glucose intolerance was reduced in rats exposed for 13weeks. Acute, but not subchronic ozone increased α2-macroglobulin, adiponectin and osteopontin. Time-course analysis indicated glucose intolerance at days 1 and 2 (2>1), and a recovery 18h post ozone. Leptin increased day 1 and epinephrine at all times after ozone. Ozone tended to decrease phosphorylated insulin receptor substrate-1 in liver and adipose tissues. ER stress appeared to be the consequence of ozone induced acute metabolic impairment since transcriptional markers of ER stress increased only after 2days of ozone. In conclusion, acute ozone exposure induces marked systemic metabolic impairments in BN rats of all ages, likely through sympathetic stimulation.

View Publications

Related Products

U-PLEX Long COVID Combo 1 (human)
IFN-β, IFN-γ, IL-6, Pentraxin 3 | Human
Multiplex
U-PLEX CAR-T Cell Safety Combo 1 (human)
GM-CSF, IFN-γ, IL-1β, IL-6, IL-10, MCP-1, TNF-α | Human
Multiplex
U-PLEX Diabetes Combo 1 (mouse)
C-Peptide, GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
U-PLEX Metabolic 3-Plex Combo 1 (mouse)
GLP-1 (total), Glucagon, Insulin | Mouse
Multiplex
U-PLEX Diabetes Combo 1 (human)
C-Peptide, GIP (total), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Human
Multiplex
U-PLEX Microbiome Combo 1 (human)
Ghrelin (total), GLP-1 (total), Glucagon, Insulin, Leptin, PP, PYY (total) | Human
Multiplex
U-PLEX Diabetes Combo 1 (rat)
C-Peptide, GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
U-PLEX Metabolic 3-Plex Combo 1 (rat)
GLP-1 (total), Glucagon, Insulin | Rat
Multiplex
U-PLEX TBI Combo 1 (human)
CD40L (soluble), Fractalkine, IFN-γ, IL-1β, IL-6, IL-18, MCP-1, RANKL/TNFSF11, TNF-α, VEGF-D | Human
Multiplex
U-PLEX Oncolysis Combo 1 (human)
CTLA-4, IFN-α2a, IFN-β, IL-1β, IL-2Rα, IL-6, IL-8, IP-10, LAG3, MIP-1α, PD1 (epitope 1), TIGIT | Human
Multiplex
U-PLEX Macrophage M1 Combo 1 (mouse)
IL-1β, IL-6, IL-12p70, IL-15, IL-23, IP-10, MCP-1, MIP-1α, TNF-α | Mouse
Multiplex
U-PLEX Custom Biomarker Group 1 (mouse) 384-well Assays
Mouse
Multiplex
U-PLEX Mouse IL-6 384-Well Assay
IL-6 | Mouse
Singleplex
U-PLEX NHP IL-6 384-Well Assay
IL-6 | Non-human primate
Singleplex
U-PLEX Human Adiponectin 384-Well Assay
Adiponectin | Human
Singleplex
U-PLEX Custom Metabolic Group 1 (rat) 384-well Assays
Rat
Multiplex
U-PLEX Custom Metabolic Group 1 (mouse) 384-well Assays
BAFF, BCA-1/BLC, BDNF, CD40/TNFRSF5, C-Peptide, Eotaxin, EPO, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, IFN-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27p28/IL-30, IL-31, IL-33, Insulin, IP-10, KC/GRO, Leptin, MCP-1, MCP-5/CCL12, MDC, MIP-1α, MIP-1β, MIP-2, MIP-3α, MMP-9 (total), PYY (total), RANTES, TARC, TNF-α, VEGF-A | Mouse
Multiplex
U-PLEX Rat Leptin 384-Well Assay
Leptin | Rat
Singleplex
U-PLEX Rat Insulin 384-Well Assay
Insulin | Rat
Singleplex
U-PLEX Rat Glucagon 384-Well Assay
Glucagon | Rat
Singleplex
U-PLEX Mouse Leptin 384-Well Assay
Leptin | Mouse
Singleplex
U-PLEX Mouse Insulin 384-Well Assay
Insulin | Mouse
Singleplex
U-PLEX Mouse Glucagon 384-Well Assay
Glucagon | Mouse
Singleplex
U-PLEX Biomarker Group 1 (NHP) 384-well Assays
CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, FLT3L, Fractalkine, G-CSF, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, IL-17F, IL-18, IL-22, IL-23, IP-10, I-TAC, MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-1β, MIP-3α, MIP-3β, MIP-5, SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, VEGF-A, YKL-40 | Non-human primate
Multiplex
U-PLEX Human A2M 384-Well Assay
A2M | Human
Singleplex
U-PLEX Custom Biomarker Group 3 (human) 384-well Assays
Human
Multiplex
U-PLEX Human IL-6 384-Well Assay
IL-6 | Human
Singleplex
U-PLEX Human Leptin 384-well Assay
Leptin | Human
Singleplex
U-PLEX Human Insulin 384-well Assay
Insulin | Human
Singleplex
U-PLEX Human Glucagon 384-well Assay
Glucagon | Human
Singleplex
U-PLEX Custom Biomarker Group 1 (human) 384-well Assays
CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FLT3L, Fractalkine, G-CSF, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, IP-10, I-TAC, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-1β, MIP-3α, MIP-3β, MIP-5, SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, YKL-40 | Human
Multiplex
U-PLEX Custom Metabolic Group 1 (human) 384-well Assays
BAFF, BDNF, β-NGF, C-Peptide, CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FGF-21, FGF-23, FLT3L, Fractalkine, FSH, G-CSF, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive), GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, Insulin, IP-10, Leptin, Luteinizing Hormone (LH), MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-5, PP, Proinsulin, PYY (total), SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, YKL-40 | Human
Multiplex
U-PLEX Custom Biomarker Group 3 (human) Assays
Human
Multiplex
U-PLEX Human Adiponectin Antibody Set
Adiponectin | Human
U-PLEX Human A2M Antibody Set
A2M | Human
U-PLEX Human Adiponectin Assay
Adiponectin | Human
Singleplex
U-PLEX Metabolic Combo 2 (human)
Adiponectin, ApoA1, ApoC3, Clusterin, CRP, DPPIV, NGAL/LCN2, RBP4, SHBG, sTfR-1 | Human
Multiplex
U-PLEX Kidney Injury Combo 1 (human)
A2M, Clusterin, Cystatin C, NGAL/LCN2, RBP4, Serpin A1 | Human
Multiplex
U-PLEX Human A2M Assay
A2M | Human
Singleplex
S-PLEX Proinflammatory Panel 1 (human) Kit
IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17A, TNF-α | Human
Multiplex
S-PLEX NHP IL-6 Kit
IL-6 | Non-human primate
Singleplex
S-PLEX Human IL-6 Kit
IL-6 | Human
Singleplex
U-PLEX Macrophage M1 Combo 1 (human)
IL-1β, IL-6, IL-12p70, IL-18, IL-23, IP-10, MCP-1, MIP-1α, TNF-α | Human
Multiplex
Glucagon Antibody
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
Human Leptin Antibody
Leptin | Human
Human Insulin Antibody
Insulin | Human, Non-human primate, Canine
V-PLEX Metabolic Panel 1 Human Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, Leptin, PP | Human
Multiplex
V-PLEX Metabolic Panel 1 Canine Kit
GIP (active), GLP-1 (active), Glucagon, Insulin | Canine
Multiplex
V-PLEX Plus Metabolic Panel 1 NHP Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, PP | Non-human primate
Multiplex
V-PLEX Plus Metabolic Panel 1 Mouse Kit
GLP-1 (active), Glucagon | Mouse
Multiplex
V-PLEX Plus Metabolic Panel 1 Human Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, Leptin, PP | Human
Multiplex
V-PLEX Plus Metabolic Panel 1 Canine Kit
GIP (active), GLP-1 (active), Glucagon, Insulin | Canine
Multiplex
V-PLEX Metabolic Panel 1 Rat Kit
GIP (active), GLP-1 (active), Glucagon | Rat
Multiplex
V-PLEX Metabolic Panel 1 NHP Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, PP | Non-human primate
Multiplex
V-PLEX Metabolic Panel 1 Mouse Kit
GLP-1 (active), Glucagon | Mouse
Multiplex
V-PLEX Plus Metabolic Panel 1 Rat Kit
GIP (active), GLP-1 (active), Glucagon | Rat
Multiplex
V-PLEX Plus Human Insulin Kit
Insulin | Human, Non-human primate, Canine
Singleplex
V-PLEX Plus Glucagon Kit
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
Singleplex
V-PLEX Human Insulin Kit
Insulin | Human, Non-human primate, Canine
Singleplex
V-PLEX Glucagon Kit
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
Singleplex
V-PLEX Plus Human Leptin Kit
Leptin | Human
Singleplex
V-PLEX Human Leptin Kit
Leptin | Human
Singleplex
V-PLEX Viral Panel 3 NHP Kit
IFN-γ, IL-1β, IL-5, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Viral Panel 3 Human Kit
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α | Human
Multiplex
V-PLEX Plus Viral Panel 3 NHP Kit
IFN-γ, IL-1β, IL-5, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Plus Viral Panel 3 Human Kit
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α | Human
Multiplex
U-PLEX Viral Combo 1 (NHP)
G-CSF, GM-CSF, IFN-α2a, IFN-γ, IL-1β, IL-1RA, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IP-10, MCP-1, MIP-1α, TNF-α, VEGF-A | Non-human primate
Multiplex
U-PLEX Viral Combo 1 (human)
G-CSF, GM-CSF, IFN-α2a, IFN-β, IFN-γ, IL-1β, IL-1RA, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IP-10, MCP-1, MIP-1α, TNF-α, VEGF-A | Human
Multiplex
V-PLEX Viral Panel 2 NHP Kit
IFN-γ, IL-1β, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Viral Panel 2 Human Kit
IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-10, TNF-α | Human
Multiplex
V-PLEX Viral Panel 1 NHP Kit
IL-1β, IL-6, IL-8 | Non-human primate
Multiplex
V-PLEX Viral Panel 1 Human Kit
IL-1β, IL-6, IL-8, TNF-α | Human
Multiplex
V-PLEX Plus Viral Panel 2 NHP Kit
IFN-γ, IL-1β, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Plus Viral Panel 2 Human Kit
IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-10, TNF-α | Human
Multiplex
V-PLEX Plus Viral Panel 1 NHP Kit
IL-1β, IL-6, IL-8 | Non-human primate
Multiplex
V-PLEX Plus Viral Panel 1 Human Kit
IL-1β, IL-6, IL-8, TNF-α | Human
Multiplex
U-PLEX Human IL-6 Assay
IL-6 | Human
Singleplex
U-PLEX Mouse IL-6 Assay
IL-6 | Mouse
Singleplex
U-PLEX NHP IL-6 Assay
IL-6 | Non-human primate
Singleplex
U-PLEX Human Leptin Assay
Leptin | Human
Singleplex
U-PLEX Human Insulin Assay
Insulin | Human
Singleplex
U-PLEX Human Glucagon Assay
Glucagon | Human
Singleplex
U-PLEX Rat Leptin Assay
Leptin | Rat
Singleplex
U-PLEX Rat Insulin Assay
Insulin | Rat
Singleplex
U-PLEX Rat Glucagon Assay
Glucagon | Rat
Singleplex
U-PLEX Mouse Leptin Assay
Leptin | Mouse
Singleplex
U-PLEX Mouse Insulin Assay
Insulin | Mouse
Singleplex
U-PLEX Mouse Glucagon Assay
Glucagon | Mouse
Singleplex
R-PLEX Human AGP Assay
AGP | Human
Singleplex
R-PLEX Mouse Adiponectin Assay
Adiponectin | Mouse
Singleplex
R-PLEX Rat IL-6 Assay
IL-6 | Rat
Singleplex
R-PLEX Rat Adiponectin Assay
Adiponectin | Rat
Singleplex
U-PLEX Custom Metabolic Group 1 (rat) Assays
Rat
Multiplex
U-PLEX Custom Metabolic Group 1 (mouse) Assays
Mouse
Multiplex
U-PLEX Gut Hormone Combo 1 (mouse)
Ghrelin (active), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
U-PLEX Adipokine Combo 1 (mouse)
BDNF, IL-1β, IL-6, IL-10, Insulin, Leptin, MCP-1, TNF-α | Mouse
Multiplex
U-PLEX Metabolic Hormones Combo 1 (rat)
C-Peptide, Ghrelin (active), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
U-PLEX Metabolic Combo 1 (rat)
BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
U-PLEX Metabolic Combo 1 (mouse)
BAFF, BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
U-PLEX Metabolic 3-Plex Combo 2 (rat)
GLP-1 (active), Glucagon, Insulin | Rat
Multiplex
Product Search
Browse Our Products

By Analytes
By Applications
Search
Customer Service/Orders


Scientific/Technical Support


Instrument Support


Company Headquarters