Choline deficiency attenuates body weight gain and improves glucose tolerance in ob/ob mice.

Wu G, Zhang L, Li T, Lopaschuk G, Vance DE, Jacobs RL.
Journal   J Obes.
Species  
Analytes Measured   Glucagon , Insulin
Matrix Tested   Plasma
Year   2012
Volume   2012
Page Numbers   319172
Application   Metabolic
Abstract
Previous studies demonstrated that choline supply is directly linked to high-fat-diet-induced obesity and insulin resistance in mice. The aim of this study was to evaluate if choline supply could also modulate obesity and insulin resistance caused by a genetic defect. Eight-week-old male ob/ob mice were fed for two months with either choline-deficient or choline-supplemented diet. Tissue weight including fat mass and lean mass was assessed. Intracellular signaling, plasma glucagon and insulin, and glucose and insulin tolerance tests were also investigated. The choline-deficient diet slowed body weight gain and decreased fat mass. Choline deficiency also decreased plasma glucose level and improved glucose and insulin tolerance although fatty liver was exacerbated. Increased adipose lipolytic activity, decreased plasma glucagon and reduced expression of hepatic glucagon receptor were also observed with the choline-deficient diet. Our results demonstrate that a choline-deficient diet can decrease fat mass and improve glucose tolerance in obese and diabetic mice caused by a genetic defect.

View Publications

Related Products

U-PLEX Diabetes Combo 2 (human)
C-Peptide, GIP (total), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) | Human
Multiplex
NEW
U-PLEX Custom Metabolic Group 1 (human) Assays
BAFF, BDNF, β-NGF, C-Peptide, CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FGF-21, FGF-23, FLT3L, Fractalkine, FSH, G-CSF, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive), GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, Insulin, IP-10, Leptin, Luteinizing Hormone (LH), MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-5, PP, Proinsulin, PYY (total), SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, YKL-40 | Human
Multiplex
NEW
U-PLEX Custom Metabolic Group 1 (mouse) Assays
BAFF, BCA-1/BLC, BDNF, CD40/TNFRSF5, C-Peptide, Eotaxin, EPO, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, IFN-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27p28/IL-30, IL-31, IL-33, Insulin, IP-10, KC/GRO, Leptin, MCP-1, MCP-5/CCL12, MDC, MIP-1α, MIP-1β, MIP-2, MIP-3α, MMP-9 (total), PYY (total), RANTES, TARC, TNF-α, VEGF-A | Mouse
Multiplex
NEW
U-PLEX Custom Metabolic Group 1 (rat) Assays
BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
NEW
U-PLEX Adipokine Combo 1 (human)
BDNF, β-NGF, IL-1β, IL-6, IL-8, IL-10, Insulin, Leptin, MCP-1, TNF-α | Human
Multiplex
NEW
U-PLEX Adipokine Combo 1 (mouse)
BDNF, IL-1β, IL-6, IL-10, Insulin, Leptin, MCP-1, TNF-α | Mouse
Multiplex
NEW
U-PLEX Diabetes Combo 1 (human)
C-Peptide, GIP (total), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Human
Multiplex
NEW
U-PLEX Diabetes Combo 1 (mouse)
C-Peptide, GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
NEW
U-PLEX Diabetes Combo 1 (rat)
C-Peptide, GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
NEW
U-PLEX Gut Hormone Combo 1 (mouse)
Ghrelin (active), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
NEW
U-PLEX Metabolic 2-Plex Combo 1 (human)
Insulin, Leptin | Human
Multiplex
NEW
U-PLEX Metabolic 2-Plex Combo 1 (mouse)
Insulin, Leptin | Mouse
Multiplex
NEW
U-PLEX Metabolic 2-Plex Combo 1 (rat)
Insulin, Leptin | Rat
Multiplex
NEW
U-PLEX Metabolic 2-Plex Combo 2 (mouse)
Glucagon, Insulin | Mouse
Multiplex
NEW
U-PLEX Metabolic 2-Plex Combo 2 (rat)
Glucagon, Insulin | Rat
Multiplex
NEW
U-PLEX Metabolic 3-Plex Combo 1 (human)
GLP-1 (active), Glucagon, Insulin | Human
Multiplex
NEW
U-PLEX Metabolic 3-Plex Combo 1 (mouse)
GLP-1 (total), Glucagon, Insulin | Mouse
Multiplex
NEW
U-PLEX Metabolic 3-Plex Combo 1 (rat)
GLP-1 (total), Glucagon, Insulin | Rat
Multiplex
NEW
U-PLEX Metabolic 3-Plex Combo 2 (mouse)
GLP-1 (active), Glucagon, Insulin | Mouse
Multiplex
NEW
U-PLEX Metabolic 3-Plex Combo 2 (rat)
GLP-1 (active), Glucagon, Insulin | Rat
Multiplex
NEW
U-PLEX Metabolic 4-Plex Combo 1 (human)
GLP-1 (active), Glucagon, Insulin, Leptin | Human
Multiplex
NEW
U-PLEX Metabolic Combo 1 (human)
BAFF, BDNF, β-NGF, C-Peptide, FGF-21, FGF-23, FSH, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive), GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, Luteinizing Hormone (LH), PP, Proinsulin, PYY (total) | Human
Multiplex
NEW
U-PLEX Metabolic Combo 1 (mouse)
BAFF, BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
NEW
U-PLEX Metabolic Combo 1 (rat)
BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
NEW
U-PLEX Metabolic Hormone Combo 1 (mouse)
C-Peptide, Ghrelin (active), GLP-1 (active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PYY (total), TNF-α | Mouse
Multiplex
NEW
U-PLEX Metabolic Hormones Combo 1 (rat)
C-Peptide, Ghrelin (active), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
NEW
U-PLEX Microbiome Combo 1 (human)
Ghrelin (total), GLP-1 (total), Glucagon, Insulin, Leptin, PP, PYY (total) | Human
Multiplex
NEW
U-PLEX Obesity Combo 1 (human)
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin | Human
Multiplex
NEW
U-PLEX Obesity Combo 1 (mouse)
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin | Mouse
Multiplex
NEW
U-PLEX Obesity Combo 1 (rat)
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin | Rat
Multiplex
NEW
U-PLEX Obesity Combo 2 (human)
C-Peptide, FGF-23, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) | Human
Multiplex
NEW
U-PLEX Obesity Combo 2 (mouse)
C-Peptide, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) | Mouse
Multiplex
NEW
U-PLEX Obesity Combo 2 (rat)
C-Peptide, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) | Rat
Multiplex
NEW
V-PLEX Custom Human Biomarkers 1
C-Peptide, CRP, Eotaxin, Eotaxin-3, FGF (basic), GIP (active), GLP-1 (active), Glucagon, GM-CSF, ICAM-1, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, Insulin, IP-10, Leptin, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, PlGF, PP, SAA, TARC, Tie-2, TNF-α, TNF-β, TSLP, VCAM-1, VEGF-A, VEGF-C, VEGF-D, VEGFR-1/Flt-1 | Human
Multiplex
V-PLEX Custom NHP Biomarkers 1
C-Peptide, Eotaxin, Eotaxin-3, GIP (active), GLP-1 (active), Glucagon, GM-CSF, IFN-γ, IL-1β, IL-2, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12/IL-23p40, IL-15, IL-16, IL-17A, Insulin, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, PP, TARC, TNF-β, VEGF-A | Non-human primate
Multiplex
U-PLEX Custom Metabolic Group 1 (ms) 384-well Assays
BAFF, BCA-1/BLC, BDNF, CD40/TNFRSF5, C-Peptide, Eotaxin, EPO, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, IFN-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27p28/IL-30, IL-31, IL-33, Insulin, IP-10, KC/GRO, Leptin, MCP-1, MCP-5/CCL12, MDC, MIP-1α, MIP-1β, MIP-2, MIP-3α, MMP-9 (total), PYY (total), RANTES, TARC, TNF-α, VEGF-A | Mouse
Multiplex
U-PLEX Custom Metabolic Group 1 (rat) 384-well Assays
BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
U-PLEX Mouse Glucagon 384-Well Assay
Glucagon | Mouse
Singleplex
U-PLEX Mouse Insulin 384-Well Assay
Insulin | Mouse
Singleplex
U-PLEX Rat Glucagon 384-Well Assay
Glucagon | Rat
Singleplex
U-PLEX Rat Insulin 384-Well Assay
Insulin | Rat
Singleplex
U-PLEX Custom Metabolic Group 1 (human) 384-well Assays
BAFF, BDNF, β-NGF, C-Peptide, CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FGF-21, FGF-23, FLT3L, Fractalkine, FSH, G-CSF, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive), GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, Insulin, IP-10, Leptin, Luteinizing Hormone (LH), MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-5, PP, Proinsulin, PYY (total), SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, YKL-40 | Human
Multiplex
U-PLEX Human Glucagon 384-well Assay
Glucagon | Human
Singleplex
U-PLEX Human Insulin 384-well Assay
Insulin | Human
Singleplex
Glucagon Antibody
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
Human Insulin Antibody
Insulin | Human, Non-human primate, Canine
V-PLEX Glucagon Kit
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
Singleplex
V-PLEX Human Insulin Kit
Insulin | Human, Non-human primate, Canine
Singleplex
V-PLEX Metabolic Panel 1 Canine Kit
GIP (active), GLP-1 (active), Glucagon, Insulin | Canine
Multiplex
V-PLEX Metabolic Panel 1 Human Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, Leptin, PP | Human
Multiplex
V-PLEX Metabolic Panel 1 Mouse Kit
GLP-1 (active), Glucagon | Mouse
Multiplex
V-PLEX Metabolic Panel 1 NHP Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, PP | Non-human primate
Multiplex
V-PLEX Metabolic Panel 1 Rat Kit
GIP (active), GLP-1 (active), Glucagon | Rat
Multiplex
V-PLEX Plus Glucagon Kit
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
Singleplex
V-PLEX Plus Human Insulin Kit
Insulin | Human, Non-human primate, Canine
Singleplex
V-PLEX Plus Metabolic Panel 1 Canine Kit
GIP (active), GLP-1 (active), Glucagon, Insulin | Canine
Multiplex
V-PLEX Plus Metabolic Panel 1 Human Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, Leptin, PP | Human
Multiplex
V-PLEX Plus Metabolic Panel 1 Mouse Kit
GLP-1 (active), Glucagon | Mouse
Multiplex
V-PLEX Plus Metabolic Panel 1 NHP Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, PP | Non-human primate
Multiplex
V-PLEX Plus Metabolic Panel 1 Rat Kit
GIP (active), GLP-1 (active), Glucagon | Rat
Multiplex
U-PLEX Human Glucagon Assay
Glucagon | Human
Singleplex
U-PLEX Human Insulin Assay
Insulin | Human
Singleplex
U-PLEX Mouse Glucagon Assay
Glucagon | Mouse
Singleplex
U-PLEX Mouse Insulin Assay
Insulin | Mouse
Singleplex
U-PLEX Rat Glucagon Assay
Glucagon | Rat
Singleplex
U-PLEX Rat Insulin Assay
Insulin | Rat
Singleplex
U-PLEX Mouse/Rat Insulin Antibody Set
Insulin | Mouse, Rat
U-PLEX Glucagon Antibody Set (5 Plate Size)
Glucagon | Human, Mouse, Rat
U-PLEX Human Insulin Antibody Set
Insulin | Human
Mouse Metabolic Kit
Insulin, Leptin | Mouse
Multiplex
Mouse/Rat Insulin Kit
Insulin | Mouse, Rat
Singleplex
Human Insulin Kit
Insulin | Human
Singleplex
V-PLEX Custom Canine Biomarkers
GIP (active), GLP-1 (active), Glucagon, Insulin | Canine
Multiplex
Browse Our Products

By Analytes
By Applications
Search
Customer Service/Orders


Scientific/Technical Support


Instrument Support


Company Headquarters