Barrier responses of human bronchial epithelial cells to grass pollen exposure.

Blume C, Swindle EJ, Dennison P, Jayasekera NP, Dudley S, Monk P, Behrendt H, Schmidt-Weber CB, Holgate ST, Howarth PH, Traidl-Hoffmann C, Davies DE.
Journal   Eur Respir J.
Species  
Analytes Measured   Eotaxin , GM-CSF , I-TAC , IP-10 , MCP-1 , MDC , RANTES , TARC
Matrix Tested   Cell culture supernatants
Year   2012
Volume  
Page Numbers  
Application   Cytokines and Chemokines
Abstract
The airway epithelium forms a physical, chemical and immunological barrier against inhaled environmental substances. In asthma, these barrier properties are thought to be abnormal. Here, we analysed the effect of grass pollen on the physical and immunological barrier properties of differentiated human primary bronchial epithelial cells.Following exposure to Timothy grass (Phleum pratense) pollen extract, the integrity of the physical barrier was not impaired as monitored by measuring the transepithelial resistance and immunofluorescence staining of tight junction proteins. In contrast, pollen exposure affected the immunological barrier properties by modulating vectorial mediator release. CXCL8/IL-8 showed the greatest increase in response to pollen exposure with preferential release to the apical compartment. Inhibition of the ERK1/2 and p38 MAPK pathways selectively blocked apical CXCL8/IL-8 release via a post transcriptional mechanism. Apical release of CCL20/MIP-3α, CCL22/MDC and TNFα was significantly increased only in severe asthma cultures, while CCL11/eotaxin-1 and CXCL10/IP-10 were reduced in non-asthmatic cultures.The bronchial epithelial barrier modulates polarised release of mediators in response to pollen without direct effects on its physical barrier properties. The differential response of cells from normal and asthmatic donors suggests the potential for the bronchial epithelium to promote immune dysfunction in asthma.

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