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Technology

Booster Pack assay development tools use MSD technology to provide a rapid and convenient method for developing signal-enhanced ECL assays on the MSD platform with users' antibody pairs and reagents. The Booster Pack reagents are compatible with assays run on streptavidin plates but not on pre-coated assay plates. In some cases, the enhanced signal shifts the dynamic range of assays, increases sensitivity, and reduces the amount of sample required.

Using the Booster Pack assay development tools to develop an immunoassay with enhanced signals requires biotinylated capture and SULFO-TAG detection antibodies. The biotinylated capture antibody binds to MSD GOLD Small Spot Streptavidin 96-well plates, where analytes in the sample interact with the capture reagents. The detection antibody, conjugated with electrochemiluminescent labels (MSD SULFO-TAG), then binds to the analytes, completing the sandwich immunoassay.

The Booster Pack assay development tools are then employed on the sandwich immunoassay, with the Booster Pack reagents facilitating signal enhancement from the SULFO-TAG antibody.

Figure 1: Assay development on an MSD GOLD 96-well Small Spot Streptavidin Plate using the Booster Pack assay development tool.

Performance

Similar to any immunoassay platform, the performance of assays enhanced with the Booster Pack assay development tool depends, in part, on the quality of available reagents, especially antibodies, calibrators, and diluents. The improvement in sensitivity over the standard assay format is dependent on the reagents, with some analytes exhibiting greater sensitivity improvements than others (see Figures 2A and 2B).

Figure 2: Representative data from assays enhanced using the Booster Pack assay development tool. (A) Sensitivity improvement observed in representative standard assays when utilizing the Booster Pack reagents. (B) Calibration curves for an assay in both the standard and enhanced assay formats.

Reagents Needed for the Booster Pack Assay Development Tool

Reagents	Booster Pack A	Booster Pack B
Booster Pack Reagents	Click Here to Purchase	Click Here to Purchase
MSD GOLD 96-Well Small Spot Streptavidin SECTOR plate	Click Here to Purchase	Included
Biotinylated Capture Antibody	User's Reagent	User’s Reagent
Assay Calibrator	User's Reagent	User’s Reagent
SULFO-TAG Detection Antibody	User's Reagent	User’s Reagent
MSD GOLD SULFO-TAG NHS-Ester Conjugation Pack	Click Here to Purchase	Click Here to Purchase
Assay and Antibody Diluent	User's Reagent	User’s Reagent
MSD GOLD Read Buffer B	Click Here to Purchase	Included