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V-PLEX Plus Rat TNF-α Kit

V-PLEX Rat TNF-α Calibration Curve K153QWG K153QWD

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The V-PLEX platform offers analytically validated singleplex and multiplex assay kits. Developed under rigorous design control, V-PLEX kits provide accurate and reproducible results with consistency from lot to lot. The V-PLEX Plus Rat TNF-α Kit enables the measurement of TNF-α in a single-analyte format. For information on multiplexing TNF-α with other analytes, please see V-PLEX Plus Proinflammatory Panel 2 Rat Kit.

V-PLEX Plus kits include controls, wash buffer, and plate seals.

Related Key Terms:
TNFSF2, Ttumor necrosis factor alpha, DIF, cachectin, cachexia, cytokine, TNF superfamily, adipokine, inflammation, Th1, Th22, NK cells, neutrophils, mast cells, eosinophils, T cells, pyrogen, innate immunity, cancer, asthma, allergy, obesity, preeclampsia, inflammatory bowel disease, psoriasis, Alzheimer's disease, sepsis

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		TNF-α
Species		Rat
Plate Type		96-well
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
Capture Antibody		Hamster Monoclonal
Detection Antibody		Goat Polyclonal
LLOD (Sensitivity)		0.72 pg/mL
Dynamic Range		0.72 - 793 pg/mL
Sample Type		Serum, Plasma, Urine, Cell Culture Supernatant
Recombinant standards		Expressed in E. coli
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component
Gene ID(s)		24835
UniProt ID(s)		P16599
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K153QWG-1

Product Description	Storage	Quantity per Kit
Proinflammatory Panel 1 Rat Control 1	2-8 °C	1 vial (1 vial)

Proinflammatory Panel 1 Rat Control 2	2-8 °C	1 vial (1 vial)

Proinflammatory Panel 1 Rat Control 3	2-8 °C	1 vial (1 vial)

Proinflammatory Panel 1 (rat) Calibrator Blend	2-8 °C	1 vial (1 vial)

SULFO-TAG Rat TNF-α Antibody	2-8 °C	1 vial (75 μL)

Rat TNF-α Plate	2-8 °C	1 each (1 plate)

Diluent Assembly 7 (includes Diluent 40 – 5 mL, Diluent 42 – 10 mL)	≤-10 °C	1 each

Wash Buffer 20X	RT	1 bottle (100 mL)

Read Buffer T 4X	RT	1 bottle (50 mL)

Blocker H, 20 mL	RT	1 bottle (20 mL)

Kit Contents for Catalog No. K153QWG-2

Product Description	Storage	Quantity per Kit
Proinflammatory Panel 1 Rat Control 1	2-8 °C	5 vial (1 vial)

Proinflammatory Panel 1 Rat Control 2	2-8 °C	5 vial (1 vial)

Proinflammatory Panel 1 Rat Control 3	2-8 °C	5 vial (1 vial)

Proinflammatory Panel 1 (rat) Calibrator Blend	2-8 °C	5 vial (1 vial)

SULFO-TAG Rat TNF-α Antibody	2-8 °C	1 vial (375 μL)

Rat TNF-α Plate	2-8 °C	5 each (1 plate)

Diluent Assembly 8 (includes Diluent 40 – 25 mL, Diluent 42 – 50 mL)	≤-10 °C	1 each

Wash Buffer 20X	RT	1 bottle (100 mL)

Read Buffer T 4X	RT	1 bottle (50 mL)

Blocker H, 100 mL	RT	1 bottle (100 mL)

Kit Contents for Catalog No. K153QWG-4

Product Description	Storage	Quantity per Kit
Proinflammatory Panel 1 Rat Control 1	2-8 °C	25 vial (1 vial)

Proinflammatory Panel 1 Rat Control 2	2-8 °C	25 vial (1 vial)

Proinflammatory Panel 1 Rat Control 3	2-8 °C	25 vial (1 vial)

Proinflammatory Panel 1 (rat) Calibrator Blend	2-8 °C	25 vial (1 vial)

SULFO-TAG Rat TNF-α Antibody	2-8 °C	5 vial (375 μL)

Rat TNF-α Plate	2-8 °C	25 each (1 plate)

Diluent Assembly 8 (includes Diluent 40 – 25 mL, Diluent 42 – 50 mL)	≤-10 °C	5 each

Wash Buffer 20X	RT	5 bottle (100 mL)

Read Buffer T 4X	RT	5 bottle (50 mL)

Blocker H, 100 mL	RT	5 bottle (100 mL)

Related Products

Product Name	Applications	Species	Plate Type
V-PLEX Rat TNF-α Kit	Cytokines & Chemokines, Immunology/Inflammation	Rat	96-well

Order Details

K153QWG

Select your instrument:

S 600

SQ 120

Q 60

Catalog No

Size

Price in USD

Qty

K153QWG-1

1 plate size

$853.00

Usually ships in 1-3 days

K153QWG-2

5 plate size

$3,138.00

Usually ships in 1-3 days

K153QWG-4

25 plate size

$14,122.00

Usually ships in 1-3 days

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		V-PLEX Proinflammatory Panel 1 Rat Calibrator Insert V-PLEX Proinflammatory Panel 1 Rat Control Pack Insert V-PLEX Proinflammatory Panel 2 Rat Product Insert
Certificate of analysis		K0082508 K0082443 with Extension Memo K0082344 K0082193 K0082192 K0082191 K0081957 K0081822 K0081509 K0081495 View More
SDS		Diluent 40 and Diluent 42 MSD Calibrators and Controls Read Buffer T (4x), Read Buffer S (4x) and Read Buffer S (1x) Wash Buffer (20X)

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Multisystem inflammatory syndrome in children and COVID-19 are distinct presentations of SARS–CoV-2 Caroline Diorio, Sarah E. Henrickson, Laura A. Vella, Kevin O. McNerney, Julie Chase, Chakkapong Burudpakdee, Jessica H. Lee, Cristina Jasen, Fran Balamuth, David M. Barrett, Brenda L. Banwell, Kathrin M. Bernt, Allison M. Blatz, Kathleen Chiotos, Brian T. Fisher, Julie C. Fitzgerald, Jeffrey S. Gerber, Kandace Gollomp, Christopher Gray, Stephan A. Grupp, Rebecca M. Harris, Todd J. Kilbaugh, Audrey R. Odom John, Michele Lambert, Emily J. Liebling, Michele E. Paessler, Whitney Petrosa, Charles Phillips, Anne F. Reilly, Neil D. Romberg, Alix Seif, Deborah A. Sesok-Pizzini, Kathleen E. Sullivan, Julie Vardaro, Edward M. Behrens, David T. Teachey, Hamid Bassiri	The Journal of Clinical Investigation	2020
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V-PLEX Plus Rat TNF-α Kit

Kit Contents for Catalog No. K153QWG-1

Kit Contents for Catalog No. K153QWG-2

Kit Contents for Catalog No. K153QWG-4

Related Products

Order Details

K153QWG

S 600

SQ 120

Q 60

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