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NEW V-PLEX Human TNF-α V2 Kit

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This assay uses a simplified protocol: incubate and read in under 3 hours. The V-PLEX Human TNF-α V2 Kit enables the measurement of TNF-α in a single-analyte format. For information on multiplexing TNF-α with other analytes, please see V-PLEX Cytolytic Panel 1 (human) Kit or V-PLEX Immune Response Panel 1 (human) Kit.

Related Key Terms:
Tumor necrosis factor, TNF, Tumor necrosis factor alpha, TNFα, cachexin, cachectin, TNF alpha, TNF-alpha, TNF-a, TNFalpha, TNFa, TNF alpha, TNFα, TNF α, Tumor necrosis factor a, Tumor necrosis factor ligand superfamily member 2, Tnfsf2, cachectin

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		TNF-α
Species		Human
Plate Type		96-well
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO QuickPlex Q 60MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
LLOD (Sensitivity)		0.25 pg/mL
Dynamic Range		0.25 - 1,360 pg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		7124
UniProt ID(s)		P01375
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151AVGD-1

Product Description	Storage	Quantity per Kit
Cytolytic Panel 1 (human) Calibrator Blend	≤-70 °C	1 vial (1 vial)

SULFO-TAG Human TNF-α Antibody, 1 Plate	2-8 °C	1 vial (75 μL)

Human TNF-α V2 96-Well Small Spot SECTOR Plate	2-8 °C	1 each (1 each)

Diluent Assembly 37 (includes Diluent 58 - 10 mL)	≤-10 °C	1 each (1 each)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

Kit Contents for Catalog No. K151AVGD-2

Product Description	Storage	Quantity per Kit
Cytolytic Panel 1 (human) Calibrator Blend	≤-70 °C	5 vial (1 vial)

SULFO-TAG Human TNF-α Antibody, 5 Plate	2-8 °C	1 vial (375 μL)

Human TNF-α V2 96-Well Small Spot SECTOR Plate	2-8 °C	5 each (1 each)

Diluent Assembly 38 (includes Diluent 58 - 50 mL)	≤-10 °C	1 each (1 each)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

Kit Contents for Catalog No. K151AVGD-4

Product Description	Storage	Quantity per Kit
Cytolytic Panel 1 (human) Calibrator Blend	≤-70 °C	25 vial (1 vial)

SULFO-TAG Human TNF-α Antibody, 5 Plate	2-8 °C	5 vial (375 μL)

Human TNF-α V2 96-Well Small Spot SECTOR Plate	2-8 °C	25 each (1 each)

Diluent Assembly 38 (includes Diluent 58 - 50 mL)	≤-10 °C	5 each (1 each)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

Kit Contents for Catalog No. K151AVGD-21

Product Description	Storage	Quantity per Kit
Cytolytic Panel 1 (human) Calibrator Blend	≤-70 °C	1 vial (1 vial)

SULFO-TAG Human TNF-α Antibody, 1 Plate	2-8 °C	1 vial (75 μL)

Cytolytic Panel 1 (hu) 96-Well 10-Spot QuickPlex Ultra Plate	2-8 °C	1 each (1 each)

Diluent Assembly 37 (includes Diluent 58 - 10 mL)	≤-10 °C	1 each (1 each)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

Kit Contents for Catalog No. K151AVGD-22

Product Description	Storage	Quantity per Kit
Cytolytic Panel 1 (human) Calibrator Blend	≤-70 °C	5 vial (1 vial)

SULFO-TAG Human TNF-α Antibody, 5 Plate	2-8 °C	1 vial (375 μL)

Cytolytic Panel 1 (hu) 96-Well 10-Spot QuickPlex Ultra Plate	2-8 °C	5 each (1 each)

Diluent Assembly 38 (includes Diluent 58 - 50 mL)	≤-10 °C	1 each (1 each)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

Kit Contents for Catalog No. K151AVGD-24

Product Description	Storage	Quantity per Kit
Cytolytic Panel 1 (human) Calibrator Blend	≤-70 °C	25 vial (1 vial)

SULFO-TAG Human TNF-α Antibody, 5 Plate	2-8 °C	5 vial (375 μL)

Cytolytic Panel 1 (hu) 96-Well 10-Spot QuickPlex Ultra Plate	2-8 °C	25 each (1 each)

Diluent Assembly 38 (includes Diluent 58 - 50 mL)	≤-10 °C	5 each (1 each)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Multisystem inflammatory syndrome in children and COVID-19 are distinct presentations of SARS–CoV-2 Caroline Diorio, Sarah E. Henrickson, Laura A. Vella, Kevin O. McNerney, Julie Chase, Chakkapong Burudpakdee, Jessica H. Lee, Cristina Jasen, Fran Balamuth, David M. Barrett, Brenda L. Banwell, Kathrin M. Bernt, Allison M. Blatz, Kathleen Chiotos, Brian T. Fisher, Julie C. Fitzgerald, Jeffrey S. Gerber, Kandace Gollomp, Christopher Gray, Stephan A. Grupp, Rebecca M. Harris, Todd J. Kilbaugh, Audrey R. Odom John, Michele Lambert, Emily J. Liebling, Michele E. Paessler, Whitney Petrosa, Charles Phillips, Anne F. Reilly, Neil D. Romberg, Alix Seif, Deborah A. Sesok-Pizzini, Kathleen E. Sullivan, Julie Vardaro, Edward M. Behrens, David T. Teachey, Hamid Bassiri	The Journal of Clinical Investigation	2020
View All

Related Products

Product Name	Applications	Species	Plate Type
V-PLEX Plus Human TNF-α V2 Kit	Cytokines & Chemokines, Immunology/Inflammation	Human	96-well

Order Details

K151AVGD

Select your instrument:

S 600

SQ 120

Q 60

Catalog No

Size

Price in USD

Qty

K151AVGD-1

1 plate size

$594.00

Not Applicable

K151AVGD-2

5 plate size

$2,303.00

Not Applicable

K151AVGD-4

25 plate size

$9,789.00

Not Applicable

K151AVGD-21

1 plate size

$594.00

Usually ships in 1-3 days

K151AVGD-22

5 plate size

$2,303.00

Usually ships in 1-3 days

K151AVGD-24

25 plate size

$9,789.00

Usually ships in 1-3 days

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		V-PLEX Cytolytic Panel 1 Human Insert
Datasheets		V-PLEX Cytolytic Panel 1 Human Datasheet
Certificate of analysis		K0082649 K0082686
SDS		Diluent 57, Diluent 58, and Diluent 59 MSD GOLD Read Buffer B

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NEW V-PLEX Human TNF-α V2 Kit

Kit Contents for Catalog No. K151AVGD-1

Kit Contents for Catalog No. K151AVGD-2

Kit Contents for Catalog No. K151AVGD-4

Kit Contents for Catalog No. K151AVGD-21

Kit Contents for Catalog No. K151AVGD-22

Kit Contents for Catalog No. K151AVGD-24

Related Products

Order Details

K151AVGD

S 600

SQ 120

Q 60

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