V-PLEX Human Biomarker 53-Plex Kit

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The V-PLEX platform offers analytically validated singleplex and multiplex assay kits. Developed under rigorous design control, V-PLEX kits provide accurate and reproducible results with consistency from lot to lot. The V-PLEX Human Biomarker 53-Plex Kit includes 53 biomarkers involved in inflammation, chemotaxis, angiogenesis, and immune system regulation (Table 1). The assays can be used for quantitative measurements in the sample types listed in the “Specifications” tab below and offer detection limits (LLODs and LLOQs) in the sub- to low pg/mL range.

Table 1. List of analytes and typical detection limits

Analyte	LLOD	LLOQ	ULOQ	Dynamic Range	Unit
CRP	1.33	27.6	49,600	1.33 - 49,600	pg/mL
Eotaxin	0.20	2.14	910	0.20 - 910	pg/mL
Eotaxin-3	1.44	11.1	11,100	1.44 - 11,100	pg/mL
FGF (basic)	0.09	2.6	1,780	0.09 - 1,780	pg/mL
GM-CSF	0.16	0.842	750	0.16 - 750	pg/mL
ICAM-1	1.94	15	32,700	1.94 - 32,700	pg/mL
IFN-γ	0.37	1.76	938	0.37 - 938	pg/mL
IL-1α	0.09	2.85	278	0.09 - 278	pg/mL
IL-1β	0.05	0.646	375	0.05 - 375	pg/mL
IL-1RA	1.12	9.19	650	1.12 - 650	pg/mL
IL-2	0.09	0.89	938	0.09 - 938	pg/mL
IL-3	2.37	12.6	1,950	2.37 - 1,950	pg/mL
IL-4	0.02	0.218	158	0.02 - 158	pg/mL
IL-5	0.14	4.41	562	0.14 - 562	pg/mL
IL-6	0.06	0.633	488	0.06 - 488	pg/mL
IL-7	0.12	0.851	563	0.12 - 563	pg/mL
IL-8	0.07	0.591	375	0.07 - 375	pg/mL
IL-9	0.311	2.23	975	0.311 - 975	pg/mL
IL-10	0.04	0.298	233	0.04 - 233	pg/mL
IL-12/IL-23p40	0.33	1.32	2,250	0.33 - 2,250	pg/mL
IL-12p70	0.11	1.22	315	0.11 - 315	pg/mL
IL-13	0.24	4.21	353	0.24 - 353	pg/mL
IL-15	0.15	0.774	525	0.15 - 525	pg/mL
IL-16	2.83	19.1	1,870	2.83 - 1,870	pg/mL
IL-17A	0.31	3.19	3,650	0.31 - 3,650	pg/mL
IL-17A Gen. B	0.413	5.86	1,950	0.413 - 1,950	pg/mL
IL-17A/F	0.93	7.57	3,900	0.93 - 3,900	pg/mL
IL-17B	0.185	1.12	1,040	0.185 - 1,040	pg/mL
IL-17C	0.682	3.84	1,620	0.682 - 1,620	pg/mL
IL-17D	3.87	11.2	5,200	3.87 - 5,200	pg/mL
IL-21	0.193	6.12	650	0.193 - 650	pg/mL
IL-22	0.27	2.78	325	0.27 - 325	pg/mL
IL-23	0.274	4.6	3,250	0.274 - 3,250	pg/mL
IL-27	4.2	38.7	13,000	4.20 - 13,000	pg/mL
IL-31	0.446	4.22	650	0.446 - 650	pg/mL
IP-10	0.12	1.29	1,240	0.12 - 1,240	pg/mL
MCP-1	0.16	1.45	247	0.16 - 247	pg/mL
MCP-4	0.05	0.518	234	0.05 - 0.518	pg/mL
MDC	1.00	6.43	2,930	1.00 - 2,930	pg/mL
MIP-1α	0.05	0.357	403	0.05 - 403	pg/mL
MIP-1β	0.09	1.20	390	0.09 - 390	pg/mL
MIP-3α	0.05	0.75	325	0.050 - 325	pg/mL
PlGF	0.21	1.5	800	0.21 - 800	pg/mL
SAA	10.9	54	13,8000	10.9 - 138,000	pg/mL
TARC	0.11	2.14	481	0.11 - 481	pg/mL
Tie-2	31.3	396	63,400	31.3 - 63,400	pg/mL
TNF-α	0.04	0.69	248	0.04 - 248	pg/mL
TNF-β	0.08	0.465	458	0.08 - 458	pg/mL
TSLP	0.063	0.46	325	0.063 - 325	pg/mL
VCAM-1	6	37.6	32,000	6.00 - 32,000	pg/mL
VEGF-A	1.12	7.7	562	1.12 - 562	pg/mL
VEGF-C	9.91	146	17,500	9.91 - 17,500	pg/mL
VEGF-D	4.36	67.1	18,800	4.36 - 18,800	pg/mL
VEGFR-1/Flt-1	0.9	10	6,410	0.90 - 6,410	pg/mL

Specifications
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		CRP, Eotaxin, Eotaxin-3, FGF (basic), GM-CSF, ICAM-1, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, MIP-3α, PlGF, SAA, TARC, Tie-2, TNF-α, TNF-β, TSLP, VCAM-1, VEGF-A, VEGF-C, VEGF-D, VEGFR-1/Flt-1
Species		Human
Instrument(s)		SECTOR Imager 2400 , SECTOR Imager 6000 , MESO QuickPlex SQ 120 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , MESO SECTOR S 600MM
Plate Type		96-well
Sample Type		Serum, Plasma, Cell Culture Supernatant, Urine
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component
Schedule B Code		3822.19.0000

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		Vascular Injury Panel 2 Human Calibrator Insert Vascular Injury Panel 2 Human Insert V-PLEX Angiogenesis Panel 1 Human Calibrator Insert V-PLEX Angiogenesis Panel 1 Human Product Insert V-PLEX Chemokine Panel 1 Human and NHP Gen. B Insert V-PLEX Chemokine Panel 1 Human Gen. B Calibrator Insert V-PLEX Cytokine Panel 1 Human Calibrator Insert View More
SDS		Blocker A Diluent 10, Diluent 11, Diluent 12, Diluent 13, Diluent 15, Diluent 16 and Diluent 17 Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 41, Diluent 43 and Diluent 45 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 Diluent 57, Diluent 58, and Diluent 59 MSD Calibrators and Controls MSD GOLD Read Buffer B Phosphate Buffer (5x) Read Buffer T (4x), Read Buffer S (4x) and Read Buffer S (1x) Wash Buffer (20X)

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
A phase 1 study of prophylactic anakinra to mitigate ICANS in patients with large B-cell lymphoma Paolo Strati, Andrew Jallouk, Qing Deng, Xubin Li, Lei Feng, Ryan Sun, Sherry Adkins, Swapna Johncy, Taylor Cain, Raphael E. Steiner, Sairah Ahmed, Dai Chihara, Luis E. Fayad, Swaminathan P. Iyer, Sandra Horowitz, Loretta J. Nastoupil, Ranjit Nair, Ahmed Hassan, Taher E. Daoud, Misha Hawkins, Maria A. Rodriguez, Elizabeth J. Shpall, Jeremy L. Ramdial, Partow Kebriaei, David S. Hong, Jason R. Westin, Sattva S. Neelapu, Michael R. Green	Blood Advances	2023
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations Bing Sun, Norina Tang, Michael J. Peluso, Nikita S. Iyer, Leonel Torres, Joanna L. Donatelli, Sadie E. Munter, Christopher C. Nixon, Rachel L. Rutishauser, Isabel Rodriguez-Barraquer, Bryan Greenhouse, John D. Kelly, Jeffrey N. Martin, Steven G. Deeks, Timothy J. Henrich, and Lynn Pulliam	Cells	2021
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
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