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U-PLEX Human TNF-α Assay

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The U-PLEX platform offers validated components that can be combined to create high quality assays quickly and easily. U-PLEX Human TNF-α Assay has the essential components for an assay that measures TNF-α in a single-analyte format. For information on multiplexing TNF-α with other analytes, please see U-PLEX Custom Biomarker Group 1 (human) Assays, U-PLEX Custom Metabolic Group 1 (human) Assays, or U-PLEX Custom Immuno-Oncology Group 1 (human) Assays.

Related Key Terms:
TNFSF2, Ttumor necrosis factor alpha, DIF, cachectin, cachexia, cytokine, TNF superfamily, adipokine, inflammation, Th1, Th22, NK cells, neutrophils, mast cells, eosinophils, T cells, pyrogen, innate immunity, cancer, asthma, allergy, obesity, preeclampsia, inflammatory bowel disease, psoriasis, Alzheimer's disease, sepsis

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		TNF-α
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO SECTOR S 600MM , SECTOR Imager 6000 , MESO QuickPlex SQ 120MM , MESO QuickPlex SQ 120 , MESO SECTOR S 600
U-PLEX Group Compatibility		Biomarker Group 1
Plate Type		96-well
Capture Antibody		Mouse Monoclonal
Detection Antibody		Goat Polyclonal
LLOD (Sensitivity)		0.51 pg/mL
Dynamic Range		0.51-3,700 pg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component
Gene ID(s)		7124
UniProt ID(s)		P01375
Schedule B Code		3822.19.0000

Product Description	Storage	Quantity per Kit
U-PLEX Human TNF-α Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

Calibrator 1	2-8 °C	1 vial (1 vial)

MSD GOLD 96 Small Spot SA Plate Pack, SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 3	≤-10 °C	1 bottle (8 mL)

Diluent 57	≤-10 °C	1 bottle (10 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

Product Description	Storage	Quantity per Kit
U-PLEX Human TNF-α Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

Calibrator 1	2-8 °C	5 vial (1 vial)

MSD GOLD 96 Small Spot SA Plate Pack, SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 3	≤-10 °C	1 bottle (40 mL)

Diluent 57	≤-10 °C	1 bottle (50 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

Product Description	Storage	Quantity per Kit
U-PLEX Human TNF-α Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

Calibrator 1	2-8 °C	25 vial (1 vial)

MSD GOLD 96 Small Spot SA Plate Pack, SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent 3	≤-10 °C	5 bottle (40 mL)

Diluent 57	≤-10 °C	5 bottle (50 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Multisystem inflammatory syndrome in children and COVID-19 are distinct presentations of SARS–CoV-2 Caroline Diorio, Sarah E. Henrickson, Laura A. Vella, Kevin O. McNerney, Julie Chase, Chakkapong Burudpakdee, Jessica H. Lee, Cristina Jasen, Fran Balamuth, David M. Barrett, Brenda L. Banwell, Kathrin M. Bernt, Allison M. Blatz, Kathleen Chiotos, Brian T. Fisher, Julie C. Fitzgerald, Jeffrey S. Gerber, Kandace Gollomp, Christopher Gray, Stephan A. Grupp, Rebecca M. Harris, Todd J. Kilbaugh, Audrey R. Odom John, Michele Lambert, Emily J. Liebling, Michele E. Paessler, Whitney Petrosa, Charles Phillips, Anne F. Reilly, Neil D. Romberg, Alix Seif, Deborah A. Sesok-Pizzini, Kathleen E. Sullivan, Julie Vardaro, Edward M. Behrens, David T. Teachey, Hamid Bassiri	The Journal of Clinical Investigation	2020
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Order Details

Catalog No

Size

Price in USD

Qty

K151UCK-1

1 plate

$485.00

Usually ships in 1-3 days

K151UCK-2

5 plates

$1,940.00

Usually ships in 1-3 days

K151UCK-4

25 plates

$8,489.00

Usually ships in 1-3 days

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		Biomarker Group 1 (human) Singleplex Insert
Datasheets		U-PLEX Human TNF-α
Product Highlights		U-PLEX Platform Highlights
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 Diluent 57, Diluent 58, and Diluent 59 MSD Calibrators and Controls MSD GOLD Read Buffer B

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U-PLEX Human TNF-α Assay

Kit Contents for Catalog No. K151UCK-1

Kit Contents for Catalog No. K151UCK-2

Kit Contents for Catalog No. K151UCK-4

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