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The
U-PLEX platform offers validated components that can be combined to create high quality assays quickly and easily. The U-PLEX Human Insulin 384-well Assay has the essential components for an assay that measures Insulin in a single-analyte format on 384-well plates. For information on multiplexing Insulin with other analytes, please see
U-PLEX Custom Metabolic Group 1 (human) Assays.
Related Key Terms: metabolic diseases, Metabolic Syndrome, Diabetes, blood sugar, beta cells, INS, IDDM, MODY, MEN1, glucose, metabolism, T1D, proinsulin, C-Peptide
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Specifications
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Kit Contents
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Product Details
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Documentation
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References
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Application(s)
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Metabolic
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Analyte(s)
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Insulin
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Species
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Human
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Instrument(s)
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MESO SECTOR S 600
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SECTOR Imager 6000
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MESO SECTOR S 600MM
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U-PLEX Group Compatibility
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Metabolic Group 1
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Plate Type
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384-well
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Capture Antibody
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Mouse monoclonal
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Detection Antibody
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Mouse monoclonal
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LLOD (Sensitivity)
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0.32 µIU/mL
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Dynamic Range
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0.32 - 736 µIU/mL
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Sample Type
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Serum, EDTA Plasma, P800 Plasma
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Gene ID(s)
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3630
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UniProt ID(s)
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P01308
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Schedule B Code
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3822.19.0000
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Product Description
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Storage
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Quantity per Kit
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2-8 °C
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1 each
(5 plate size)
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2-8 °C
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5 vial
(1 vial)
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2-8 °C
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5 each
(1 plate)
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2-8 °C
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2 bottle
(50 mL)
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≤-10 °C
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2 bottle
(50 mL)
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≤-10 °C
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2 bottle
(50 mL)
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RT
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1 bottle
(90 mL)
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Aprotinin
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2-8 °C
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2 vial
(250 μL)
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Product Description
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Storage
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Quantity per Kit
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2-8 °C
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5 each
(5 plate size)
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2-8 °C
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25 vial
(1 vial)
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2-8 °C
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25 each
(1 plate)
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2-8 °C
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10 bottle
(50 mL)
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≤-10 °C
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10 bottle
(50 mL)
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≤-10 °C
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10 bottle
(50 mL)
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RT
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5 bottle
(90 mL)
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Aprotinin
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2-8 °C
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10 vial
(250 μL)
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Notes: - The Insulin assay will cross-react with the Proinsulin assay. We do not recommend multiplexing the Insulin assay with the Proinsulin assay on the same plate.
- The U-PLEX Human Insulin Assay has been anchored to NIBSC/WHO standard 83/500. Please see the table below for conversion factors. The U-PLEX Human Insulin Assay uses a different format and protocol than previous MSD assays for insulin. In a limited study with samples comparing the U-PLEX Human Insulin Assay to previous MSD insulin assays, the observed concentrations of the samples were on average within 20% for similar protocols. The data were insufficient to support a general, single-factor conversion between the assays and we recommend that users transitioning from previous MSD insulin assays carry out a standard concordance study. Please contact MSD Scientific Support for further information.
| Analyte | U-PLEX Calibrator | NIBSC/WHO Cat. # | NIBSC/WHO Unit | MSD Unit | NIBSC/WHO : MSD |
| Insulin | Calibrator 15 | 83/500 | µIU/mL | µIU/mL | 1 |
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Product Inserts
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Datasheets
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Product Highlights
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SDS
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Title
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Journal
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Year
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Islet Autoantibody Standardization Program: interlaboratory comparison of insulin autoantibody assay performance in 2018 and 2020 workshops
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Leptin levels are associated with multiple sclerosis risk
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Effect of orally administered L. fermentum NCIMB 5221 on markers of metabolic syndrome: an in vivo analysis using ZDF rats
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Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo.
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Fibroblast growth factor 21 mediates specific glucagon actions.
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