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U-PLEX CAR-T Cell Exhaustion Combo 1 (human)

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U-PLEX Combos are multiplex assays that are related to a specific application. They have been assembled for ordering convenience. The U-PLEX CAR-T Cell Exhaustion Combo 1 (human) contains assays for seven analytes related to CAR-T cell exhaustion (Table 1). The assays can be used for quantitative measurements in the sample types listed in the "Specifications" tab below, and offer detection limits (LLODs) in the sub- to low pg/mL range.

Table 1. List of analytes and typical detection limits

Analyte	LLOD - ULOD	units
CD28	14 – 14,400	pg/mL
CTLA4	0.12 – 1,500	pg/mL
IFN-γ	1.7 – 17,000	pg/mL
LAG3	6.8 – 36,000	pg/mL
PD-L1 (epitope 1)	0.09 – 1,100	pg/mL
TIGIT	0.36 – 3,500	pg/mL
TNF-α	0.51 – 3,650	pg/mL

Specifications
Kit Contents
Documentation
References

Analyte(s)		CD28, CTLA-4, IFN-γ, LAG3, PD-L1 (epitope 1), TIGIT, TNF-α
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , SECTOR Imager 6000 , MESO SECTOR S 600MM , MESO QuickPlex SQ 120
U-PLEX Group Compatibility		Immuno-Oncology Group 1
Plate Type		96-well
Sample Type		Cell Culture Supernatant, Plasma, Serum
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component
Schedule B Code		3822.19.0000

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 7-Assay (300 µL)	2-8 °C	1 each (1 vial)

U-PLEX Human CD28 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human LAG3 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human IFN-γ Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human TNF-α Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human CTLA-4 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human TIGIT Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human PD-L1 (Epitope 1) Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

Calibrator 1	2-8 °C	1 vial (1 vial)

Calibrator 20	2-8 °C	1 vial (1 vial)

Calibrator 21	2-8 °C	1 vial (1 vial)

Calibrator 23	≤-70 °C	1 vial (1 vial)

U-PLEX 7-Assay, 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)

Diluent 3	≤-10 °C	1 bottle (8 mL)

Diluent 58, 10 mL	≤-10 °C	1 bottle (10 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 7-Assay (1.8 mL)	2-8 °C	1 each (1 vial)

U-PLEX Human CD28 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human LAG3 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human IFN-γ Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human TNF-α Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human CTLA-4 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human TIGIT Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human PD-L1 (epitope 1) Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

Calibrator 1	2-8 °C	5 vial (1 vial)

Calibrator 20	2-8 °C	5 vial (1 vial)

Calibrator 21	2-8 °C	5 vial (1 vial)

Calibrator 23	≤-70 °C	5 vial (1 vial)

U-PLEX 7-Assay, 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)

Diluent 3	≤-10 °C	1 bottle (40 mL)

Diluent 58	≤-10 °C	1 bottle (50 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 7-Assay (1.8 mL)	2-8 °C	5 each (1 vial)

U-PLEX Human CD28 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human LAG3 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human IFN-γ Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human TNF-α Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human CTLA-4 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human TIGIT Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human PD-L1 (epitope 1) Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

Calibrator 1	2-8 °C	25 vial (1 vial)

Calibrator 20	2-8 °C	25 vial (1 vial)

Calibrator 21	2-8 °C	25 vial (1 vial)

Calibrator 23	≤-70 °C	25 vial (1 vial)

U-PLEX 7-Assay, 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

U-PLEX Stop Solution	2-8 °C	5 bottle (40 mL)

Diluent 3	≤-10 °C	5 bottle (40 mL)

Diluent 58	≤-10 °C	5 bottle (50 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Multisystem inflammatory syndrome in children and COVID-19 are distinct presentations of SARS–CoV-2 Caroline Diorio, Sarah E. Henrickson, Laura A. Vella, Kevin O. McNerney, Julie Chase, Chakkapong Burudpakdee, Jessica H. Lee, Cristina Jasen, Fran Balamuth, David M. Barrett, Brenda L. Banwell, Kathrin M. Bernt, Allison M. Blatz, Kathleen Chiotos, Brian T. Fisher, Julie C. Fitzgerald, Jeffrey S. Gerber, Kandace Gollomp, Christopher Gray, Stephan A. Grupp, Rebecca M. Harris, Todd J. Kilbaugh, Audrey R. Odom John, Michele Lambert, Emily J. Liebling, Michele E. Paessler, Whitney Petrosa, Charles Phillips, Anne F. Reilly, Neil D. Romberg, Alix Seif, Deborah A. Sesok-Pizzini, Kathleen E. Sullivan, Julie Vardaro, Edward M. Behrens, David T. Teachey, Hamid Bassiri	The Journal of Clinical Investigation	2020
View All

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Product Name	Applications	Species	Plate Type
U-PLEX CAR-T Cell Combo 2 (human)	Oncology & Cancer	Human	96-well
U-PLEX CAR-T Cell Efficacy Combo 1 (human)		Human	96-well
U-PLEX CAR-T Cell Persistence Combo 1 (human)		Human	96-well
U-PLEX CAR-T Cell Safety Combo 1 (human)		Human	96-well

Order Details

Catalog No

Size

Price in USD

Qty

K15710K-1

1 plate size

$2,080.00

Planned: 3 Day Delivery

K15710K-2

5 plate size

$8,319.00

Planned: 3 Day Delivery

K15710K-4

25 plate size

$36,393.00

Planned: 3 Day Delivery

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		U-PLEX I-O Group 1 (human) Multiplex Insert
Datasheets		U-PLEX Human CD28 U-PLEX Human CTLA-4 U-PLEX Human IFN-γ U-PLEX Human LAG3 U-PLEX Human PD-L1 (epitope 1) U-PLEX Human TIGIT U-PLEX Human TNF-α
Product Highlights		U-PLEX Platform Highlights
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 57, Diluent 58, and Diluent 59 MSD Calibrators and Controls MSD GOLD Read Buffer B U-PLEX Linker Set
Technical Notes		Design of biomarker multiplex assays to support research on CAR-T cell efficacy, safety, persistence and exhaustion
Scientific Posters		Multiplexed Cytokine Assessment of T-and NK-cell Responses to Tumor Cells: Parallel Assessment of Biomarkers Associated with Efficacy, Safety, and Persistence

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U-PLEX CAR-T Cell Exhaustion Combo 1 (human)

Kit Contents for Catalog No. K15710K-1

Kit Contents for Catalog No. K15710K-2

Kit Contents for Catalog No. K15710K-4

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