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S-PLEX Human TNF-β Kit

Human TNF-b Calibrator Curve K151ADPS K156ADPS

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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human TNF-β Assay has the essential components for an ultra-sensitive assay that measures TNF-β in a single-analyte format. For detailed instructions on running the TNF-β assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
LTA, LT, TNFB, TNFSF1, Lymphotoxin alpha, LT-alpha, tnfb, TNLG1E, TNFSF1, TNFSF1B, TNFSF1TNF-beta, psoriatic arthritis

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		TNF-β
Species		Human
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO QuickPlex Q 60MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
Plate Type		96-well
Capture Antibody		Mouse monoclonal
Detection Antibody		Mouse monoclonal
LLOD (Sensitivity)		3.3 fg/mL
Dynamic Range		3.3 - 16,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant, CSF
Recombinant standards		Expressed in E. coli
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		4049
UniProt ID(s)		P01374
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151ADPS-1

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	1 each (8 mL)

S-PLEX Human TNF-β Antibody Set	2-8 °C	1 each (1 plate size)

Human TNF-β Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (8 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151ADPS-2

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	1 each (40 mL)

S-PLEX Human TNF-β Antibody Set	2-8 °C	1 each (5 plate size)

Human TNF-β Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (40 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151ADPS-4

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	5 each (40 mL)

S-PLEX Human TNF-β Antibody Set	2-8 °C	5 each (5 plate size)

Human TNF-β Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent 59	2-8 °C	5 bottle (40 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Kit Contents for Catalog No. K151ADPS-21

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	1 each (8 mL)

S-PLEX Human TNF-β Antibody Set	2-8 °C	1 each (1 plate size)

Human TNF-β Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (8 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151ADPS-22

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	1 each (40 mL)

S-PLEX Human TNF-β Antibody Set	2-8 °C	1 each (5 plate size)

Human TNF-β Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (40 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151ADPS-24

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	5 each (40 mL)

S-PLEX Human TNF-β Antibody Set	2-8 °C	5 each (5 plate size)

Human TNF-β Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent 59	2-8 °C	5 bottle (40 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Order Details

K151ADPS

Select your instrument:

S 600

SQ 120

Q 60

Catalog No

Size

Price in USD

Qty

K151ADPS-1

1 plate size

$882.00

Not Applicable

K151ADPS-2

5 plate size

$3,735.00

Not Applicable

K151ADPS-4

25 plate size

$15,873.00

Not Applicable

K151ADPS-21

1 plate size

$882.00

Not Applicable

K151ADPS-22

5 plate size

$3,735.00

Not Applicable

K151ADPS-24

25 plate size

$15,873.00

Not Applicable

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		S-PLEX TNF-b Kit Product Insert
Certificate of analysis		K00S0217 with Extension K00S0151 K00S0036
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 Diluent 57, Diluent 58, and Diluent 59 MSD GOLD Read Buffer B

Title	Journal	Year
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Biomarkers of Tuberculosis Severity and Treatment Effect: A Directed Screen of 70 Host Markers in a Randomized Clinical Trial G B Sigal, M R Segal, A Mathew, L Jarlsberg, M Wang, S Barbero, N Small, K Haynesworth, J L Davis, M Weiner, W C Whitworth, J Jacobs, J Schorey, D M Lewinsohn, P Nahid	EBioMedicine	2017
Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy. Koppes SA, Brans R, Ljubojevic Hadzavdic S, Frings-Dresen MH, Rustemeyer T, Kezic S.	Int Arch Allergy Immunol.	2016

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S-PLEX Human TNF-β Kit

Kit Contents for Catalog No. K151ADPS-1

Kit Contents for Catalog No. K151ADPS-2

Kit Contents for Catalog No. K151ADPS-4

Kit Contents for Catalog No. K151ADPS-21

Kit Contents for Catalog No. K151ADPS-22

Kit Contents for Catalog No. K151ADPS-24

Order Details

K151ADPS

S 600

SQ 120

Q 60

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