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S-PLEX Human IL-6 Kit

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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IL-6 Assay has the essential components for an ultrasensitive assay that measures IL-6 in a single-analyte format. For detailed instructions on running the IL-6 assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
IL6, BSF2, HGF, HSF, IFNB2, BSF-2, CDF, IFN-beta-2, interleukin 6, rheumatoid arthritis

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		IL-6
Species		Human
Instrument(s)		SECTOR Imager 2400 , SECTOR Imager 6000 , MESO QuickPlex SQ 120 , MESO SECTOR S 600 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600MM
Plate Type		96-well
Capture Antibody		Mouse monoclonal
Detection Antibody		Mouse monoclonal
LLOD (Sensitivity)		1.1 fg/mL
Dynamic Range		1.1 - 6,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant
Recombinant standards		Expressed in E. coli
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		3569
UniProt ID(s)		P05231
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151B3S-1

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-6 Antibody Set	2-8 °C	1 each (1 plate size)

Human IL-6 Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 21 (includes Diluent 2 – 8 mL, and Diluent 3 – 8 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	1 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (18 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151B3S-2

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-6 Antibody Set	2-8 °C	1 each (5 plate size)

Human IL-6 Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	5 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (90 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151B3S-4

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-6 Antibody Set	2-8 °C	5 each (5 plate size)

Human IL-6 Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)	≤-10 °C	5 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	25 each (50 μL)

MSD GOLD Read Buffer A	RT	5 bottle (90 mL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Order Details

Catalog No

Size

Price in USD

Qty

K151B3S-1

1 plate size

$844.00

Usually ships in 1-3 days

K151B3S-2

5 plate size

$3,574.00

Usually ships in 1-3 days

K151B3S-4

25 plate size

$14,665.00

Usually ships in 1-3 days

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		S-PLEX IL-6 Kit Product Insert
Certificate of analysis		K00S0086 K00S0065 K00S0019 with Extension memo K00S0003A
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 MSD GOLD Read Buffer A

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations Bing Sun, Norina Tang, Michael J. Peluso, Nikita S. Iyer, Leonel Torres, Joanna L. Donatelli, Sadie E. Munter, Christopher C. Nixon, Rachel L. Rutishauser, Isabel Rodriguez-Barraquer, Bryan Greenhouse, John D. Kelly, Jeffrey N. Martin, Steven G. Deeks, Timothy J. Henrich, and Lynn Pulliam	Cells	2021
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
IL-6 as a Mediator of the Association Between Traditional Risk Factors and Future Myocardial Infarction: A Nested Case-Control Study Mulugeta M Zegeye, Jonas S O Andersson, Patrik Wennberg, Dirk Repsilber, Allan Sirsjö, Liza U Ljungberg	Arteriosclerosis, Thrombosis, and Vascular Biology	2021
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S-PLEX Human IL-6 Kit

Kit Contents for Catalog No. K151B3S-1

Kit Contents for Catalog No. K151B3S-2

Kit Contents for Catalog No. K151B3S-4

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