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S-PLEX Human IL-21 Kit

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S-PLEX is MSD’s ultrasensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IL-21 Assay has the essential components for an ultrasensitive assay that measures IL-21 in a single-analyte format. For detailed instructions on running the IL-21 assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
CVID11, Za11, cytokine, Th2, Th17, interleukin, immunity, anti-viral immunity, regulatory cytokine, arthritis, colitis, Crohn's disease, Hodgkin's lymphoma

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		IL-21
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , SECTOR Imager 6000 , MESO SECTOR S 600MM , MESO QuickPlex SQ 120
Plate Type		96-well
Capture Antibody		Mouse monoclonal
Detection Antibody		Mouse monoclonal
LLOD (Sensitivity)		8.2 fg/mL
Dynamic Range		8.2 - 54,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant, CSF
Recombinant standards		Expressed in a human cell line
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		59067
UniProt ID(s)		Q9HBE4
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151ADNS-1

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	1 each (8 mL)

S-PLEX Human IL-21 Antibody Set	2-8 °C	1 each (1 plate size)

Human IL-21 Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (8 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151ADNS-2

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	1 each (40 mL)

S-PLEX Human IL-21 Antibody Set	2-8 °C	1 each (5 plate size)

Human IL-21 Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (40 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151ADNS-4

Product Description	Storage	Quantity per Kit
Diluent 2	≤-10 °C	5 each (40 mL)

S-PLEX Human IL-21 Antibody Set	2-8 °C	5 each (5 plate size)

Human IL-21 Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent 59	2-8 °C	5 bottle (40 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Order Details

Catalog No

Size

Price in USD

Qty

K151ADNS-1

1 plate size

$844.00

Not Applicable

K151ADNS-2

5 plate size

$3,574.00

Not Applicable

K151ADNS-4

25 plate size

$14,665.00

Not Applicable

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		S-PLEX Human IL-21 Kit Product Insert
Certificate of analysis		K00S0192 K00S0127 with Extension memo
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 Diluent 57, Diluent 58, and Diluent 59 MSD GOLD Read Buffer B

Title	Journal	Year
Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics Alpana Waghmare, Elizabeth M Krantz, Subhasish Baral, Emma Vasquez, Tillie Loeffelholz, E. Lisa Chung, Urvashi Pandey, Jane Kuypers, Elizabeth R Duke, Keith R Jerome, Alexander L Greninger, Daniel B Reeves, Florian Hladik, E Fabian Cardozo-Ojeda, Michael Boeckh, Joshua T Schiffer	TBD	2020
Alopecia areata is characterized by dysregulation in systemic type 17 and type 2 cytokines, which may contribute to disease-associated psychological morbidity Bain KA, McDonald E, Moffat F, Tutino M, Castelino M, Barton A, Cavanagh J, Ijaz UZ, Siebert S, McInnes IB, Astrand A, Holmes S, Milling SWF	British Journal of Dermatology	2019

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S-PLEX Human IL-21 Kit

Kit Contents for Catalog No. K151ADNS-1

Kit Contents for Catalog No. K151ADNS-2

Kit Contents for Catalog No. K151ADNS-4

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