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S-PLEX Human IL-1β Kit

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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IL-1β Assay has the essential components for an ultra-sensitive assay that measures IL-1β in a single-analyte format. For detailed instructions on running the IL-1β assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
IL1F2, catabolin, proinflammatory, cytokine, interleukin 1 cytokine family, pyrogen, inflammation, angiogenesis, Th17, Th9, autoimmunity, allergy, asthma, multiple sclerosis

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		IL-1β
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , SECTOR Imager 6000 , MESO SECTOR S 600MM , MESO QuickPlex SQ 120
Plate Type		96-well
Capture Antibody		Mouse monoclonal
Detection Antibody		Mouse monoclonal
LLOD (Sensitivity)		19 fg/mL
Dynamic Range		19 - 70,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant, CSF, Urine
Recombinant standards		Expressed in E. coli
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		3553
UniProt ID(s)		P01584
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151ADSS-1

Product Description	Storage	Quantity per Kit
Diluent 58	≤-10 °C	1 bottle (10 mL)

S-PLEX Human IL-1β Antibody Set	2-8 °C	1 each (1 plate size)

Human IL-1β Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (8 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151ADSS-2

Product Description	Storage	Quantity per Kit
Diluent 58	≤-10 °C	1 bottle (50 mL)

S-PLEX Human IL-1β Antibody Set	2-8 °C	1 each (5 plate size)

Human IL-1β Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (40 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151ADSS-4

Product Description	Storage	Quantity per Kit
Diluent 58	≤-10 °C	5 bottle (50 mL)

S-PLEX Human IL-1β Antibody Set	2-8 °C	5 each (5 plate size)

Human IL-1β Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent 59	2-8 °C	5 bottle (40 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Order Details

Catalog No

Size

Price in USD

Qty

K151ADSS-1

1 plate size

$844.00

Not Applicable

K151ADSS-2

5 plate size

$3,574.00

Not Applicable

K151ADSS-4

25 plate size

$14,665.00

Not Applicable

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		S-PLEX IL-1b Kit Product Insert
Certificate of analysis		K00S0208 K00S0214 K00S0163 K00S0134 K00S0072 with Extension memo K00S0040
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 Diluent 57, Diluent 58, and Diluent 59 MSD GOLD Read Buffer B

Title	Journal	Year
A phase 1 study of prophylactic anakinra to mitigate ICANS in patients with large B-cell lymphoma Paolo Strati, Andrew Jallouk, Qing Deng, Xubin Li, Lei Feng, Ryan Sun, Sherry Adkins, Swapna Johncy, Taylor Cain, Raphael E. Steiner, Sairah Ahmed, Dai Chihara, Luis E. Fayad, Swaminathan P. Iyer, Sandra Horowitz, Loretta J. Nastoupil, Ranjit Nair, Ahmed Hassan, Taher E. Daoud, Misha Hawkins, Maria A. Rodriguez, Elizabeth J. Shpall, Jeremy L. Ramdial, Partow Kebriaei, David S. Hong, Jason R. Westin, Sattva S. Neelapu, Michael R. Green	Blood Advances	2023
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics Alpana Waghmare, Elizabeth M Krantz, Subhasish Baral, Emma Vasquez, Tillie Loeffelholz, E. Lisa Chung, Urvashi Pandey, Jane Kuypers, Elizabeth R Duke, Keith R Jerome, Alexander L Greninger, Daniel B Reeves, Florian Hladik, E Fabian Cardozo-Ojeda, Michael Boeckh, Joshua T Schiffer	TBD	2020
Alopecia areata is characterized by dysregulation in systemic type 17 and type 2 cytokines, which may contribute to disease-associated psychological morbidity Bain KA, McDonald E, Moffat F, Tutino M, Castelino M, Barton A, Cavanagh J, Ijaz UZ, Siebert S, McInnes IB, Astrand A, Holmes S, Milling SWF	British Journal of Dermatology	2019
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S-PLEX Human IL-1β Kit

Kit Contents for Catalog No. K151ADSS-1

Kit Contents for Catalog No. K151ADSS-2

Kit Contents for Catalog No. K151ADSS-4

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