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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IL-12p70 Assay has the essential components for an ultrasensitive assay that measures IL-12p70 in a single-analyte format. For detailed instructions on running the IL-12p70 assay, please see the product insert in the "Documentation" tab below.
Related Key Terms:
NKSF1, IL-12 subunit p35, IL-12 subunit p40, IL12 p70, p35, Interleukin 12A
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IL-12p70
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Species
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Human
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Instrument(s)
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SECTOR Imager 2400
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SECTOR Imager 6000
,
MESO QuickPlex SQ 120
,
MESO SECTOR S 600
,
MESO QuickPlex SQ 120MM
,
MESO SECTOR S 600MM
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Plate Type
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96-well
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Capture Antibody
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Rat monoclonal
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Detection Antibody
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Mouse monoclonal
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LLOD (Sensitivity)
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2.3 fg/mL
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Dynamic Range
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2.3 - 35,000 fg/mL
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Sample Type
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Serum, Plasma, Cell Culture Supernatant
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Recombinant standards
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Expressed in Insect cell line
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Condition
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Multi-Component
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Gene ID(s)
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3592
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UniProt ID(s)
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O60595
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Schedule B Code
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3822.19.0000
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Product Description
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Storage
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Quantity per Kit
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S-PLEX Human IL-12p70 Antibody Set
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2-8 °C
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1 each
(1 plate size)
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Human IL-12p70 Calibrator
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2-8 °C
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1 vial
(1 vial)
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S-PLEX Coating Reagent C1
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≤-70 °C
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1 vial
(300 μL)
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S-PLEX Detect D2 (200X)
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≤-70 °C
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1 each
(50 μL)
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S-PLEX Detect D1 (4X)
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≤-70 °C
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1 each
(1.7 mL)
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S-PLEX 96-Well SECTOR Plate
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2-8 °C
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1 each
(1 plate)
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2-8 °C
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1 bottle
(50 mL)
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Diluent Assembly 21 (includes Diluent 2 – 8 mL, and Diluent 3 – 8 mL)
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≤-10 °C
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1 each
(1 each)
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S-PLEX Enhance E1 (4X)
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≤-10 °C
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1 each
(1.7 mL)
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S-PLEX Enhance E2
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≤-10 °C
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1 vial
(1.7 mL)
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S-PLEX Enhance E3 (200X)
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≤-70 °C
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1 each
(50 μL)
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RT
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1 bottle
(18 mL)
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≤-10 °C
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1 vial
(500 μL)
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Product Description
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Storage
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Quantity per Kit
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S-PLEX Human IL-12p70 Antibody Set
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2-8 °C
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1 each
(5 plate size)
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Human IL-12p70 Calibrator
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2-8 °C
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5 vial
(1 vial)
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S-PLEX Coating Reagent C1
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≤-70 °C
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1 vial
(300 μL)
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S-PLEX Detect D2 (200X)
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≤-70 °C
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5 each
(50 μL)
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S-PLEX Detect D1 (4X)
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≤-70 °C
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5 each
(1.7 mL)
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S-PLEX 96-Well SECTOR Plate
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2-8 °C
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5 each
(1 plate)
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2-8 °C
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1 bottle
(50 mL)
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Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)
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≤-10 °C
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1 each
(1 each)
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S-PLEX Enhance E1 (4X)
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≤-10 °C
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5 each
(1.7 mL)
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S-PLEX Enhance E2
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≤-10 °C
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5 vial
(1.7 mL)
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S-PLEX Enhance E3 (200X)
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≤-70 °C
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5 each
(50 μL)
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RT
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1 bottle
(90 mL)
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≤-10 °C
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1 vial
(500 μL)
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Product Description
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Storage
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Quantity per Kit
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S-PLEX Human IL-12p70 Antibody Set
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2-8 °C
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5 each
(5 plate size)
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Human IL-12p70 Calibrator
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2-8 °C
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25 vial
(1 vial)
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S-PLEX Coating Reagent C1
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≤-70 °C
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5 vial
(300 μL)
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S-PLEX Detect D2 (200X)
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≤-70 °C
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25 each
(50 μL)
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S-PLEX Detect D1 (4X)
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≤-70 °C
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25 each
(1.7 mL)
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S-PLEX 96-Well SECTOR Plate
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2-8 °C
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25 each
(1 plate)
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2-8 °C
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5 bottle
(50 mL)
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Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)
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≤-10 °C
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5 each
(1 each)
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S-PLEX Enhance E1 (4X)
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≤-10 °C
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25 each
(1.7 mL)
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S-PLEX Enhance E2
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≤-10 °C
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25 vial
(1.7 mL)
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S-PLEX Enhance E3 (200X)
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≤-70 °C
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25 each
(50 μL)
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RT
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5 bottle
(90 mL)
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≤-10 °C
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5 vial
(500 μL)
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Product Inserts
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Certificate of analysis
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SDS
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Title
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Journal
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Year
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Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection
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Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics
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Cytokine biomarkers in tear film for primary open-angle glaucoma
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A Pilot Study of Preoperative Single-Dose Ipilimumab and/or Cryoablation in Women with Early-Stage Breast Cancer with Comprehensive Immune Profiling.
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Effect of Wild-Type Shigella Species and Attenuated Shigella Vaccine Candidates on Small Intestinal Barrier Function, Antigen Trafficking, and Cytokine Release.
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