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S-PLEX Human IFN-γ Kit

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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IFN-γ Assay has the essential components for an ultra-sensitive assay that measures IFN-γ in a single-analyte format. For detailed instructions on running the IFN-γ assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
cytokine, type II Interferon, IFNG, IFG, IFI, antimicrobial, inflammation, antiviral, immunomodulatory, Th1, autoimmunity, Huntington's disease, hepatitis C

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		IFN-γ
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , SECTOR Imager 6000 , MESO SECTOR S 600MM , MESO QuickPlex SQ 120
Plate Type		96-well
Capture Antibody		Mouse monoclonal
Detection Antibody		Mouse monoclonal
LLOD (Sensitivity)		5.3 fg/mL
Dynamic Range		5.3 - 15,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant, CSF
Recombinant standards		Expressed in E. coli
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		3458
UniProt ID(s)		P01579
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151X9S-1

Product Description	Storage	Quantity per Kit
Diluent 58	≤-10 °C	1 bottle (10 mL)

S-PLEX Human IFN-γ Antibody Set	2-8 °C	1 each (1 plate size)

Human IFN-γ Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (8 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151X9S-2

Product Description	Storage	Quantity per Kit
Diluent 58	≤-10 °C	1 bottle (50 mL)

S-PLEX Human IFN-γ Antibody Set	2-8 °C	1 each (5 plate size)

Human IFN-γ Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent 59	2-8 °C	1 bottle (40 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151X9S-4

Product Description	Storage	Quantity per Kit
Diluent 58	≤-10 °C	5 bottle (50 mL)

S-PLEX Human IFN-γ Antibody Set	2-8 °C	5 each (5 plate size)

Human IFN-γ Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent 59	2-8 °C	5 bottle (40 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Title	Journal	Year
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Multisystem inflammatory syndrome in children and COVID-19 are distinct presentations of SARS–CoV-2 Caroline Diorio, Sarah E. Henrickson, Laura A. Vella, Kevin O. McNerney, Julie Chase, Chakkapong Burudpakdee, Jessica H. Lee, Cristina Jasen, Fran Balamuth, David M. Barrett, Brenda L. Banwell, Kathrin M. Bernt, Allison M. Blatz, Kathleen Chiotos, Brian T. Fisher, Julie C. Fitzgerald, Jeffrey S. Gerber, Kandace Gollomp, Christopher Gray, Stephan A. Grupp, Rebecca M. Harris, Todd J. Kilbaugh, Audrey R. Odom John, Michele Lambert, Emily J. Liebling, Michele E. Paessler, Whitney Petrosa, Charles Phillips, Anne F. Reilly, Neil D. Romberg, Alix Seif, Deborah A. Sesok-Pizzini, Kathleen E. Sullivan, Julie Vardaro, Edward M. Behrens, David T. Teachey, Hamid Bassiri	The Journal of Clinical Investigation	2020
Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics Alpana Waghmare, Elizabeth M Krantz, Subhasish Baral, Emma Vasquez, Tillie Loeffelholz, E. Lisa Chung, Urvashi Pandey, Jane Kuypers, Elizabeth R Duke, Keith R Jerome, Alexander L Greninger, Daniel B Reeves, Florian Hladik, E Fabian Cardozo-Ojeda, Michael Boeckh, Joshua T Schiffer	TBD	2020
Early and late effects of maternal experience on hippocampal neurogenesis, microglia, and the circulating cytokine milieu Rand S. Eid, Jessica A. Chaiton, Stephanie E. Lieblich, Tamara S. Bodnar, Joanne Weinberg, Liisa A.M. Galea	Neurobiology of Aging	2019
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Order Details

Catalog No

Size

Price in USD

Qty

K151X9S-1

1 plate size

$844.00

Not Applicable

K151X9S-2

5 plate size

$3,574.00

Not Applicable

K151X9S-4

25 plate size

$14,665.00

Not Applicable

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		S-PLEX Human IFN-g Kit Product Insert
Certificate of analysis		K00S0199 K00S0179 K00S0105 K00S0082 with Extension memo K00S0032
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 Diluent 57, Diluent 58, and Diluent 59 MSD GOLD Read Buffer B

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S-PLEX Human IFN-γ Kit

Kit Contents for Catalog No. K151X9S-1

Kit Contents for Catalog No. K151X9S-2

Kit Contents for Catalog No. K151X9S-4

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