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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IFN-α2a Assay has the essential components for an ultrasensitive assay that measures IFN-α2a in a single-analyte format. For detailed instructions on running the IFN-α2a assay, please see the product insert in the "Documentation" tab below.
Related Key Terms:
IFNA2, IFN-alphaA, IFNA, interferon alpha 2, hepatitis, lupus
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IFN-α2a
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Species
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Human
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Instrument(s)
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SECTOR Imager 2400
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SECTOR Imager 6000
,
MESO QuickPlex SQ 120
,
MESO SECTOR S 600
,
MESO QuickPlex SQ 120MM
,
MESO SECTOR S 600MM
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Plate Type
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96-well
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Capture Antibody
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Mouse monoclonal
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Detection Antibody
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Mouse monoclonal
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LLOD (Sensitivity)
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4.9 fg/mL
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Dynamic Range
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4.9 - 67,000 fg/mL
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Sample Type
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Serum, Plasma, Cell Culture Supernatant
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Recombinant standards
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Expressed in E. coli
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Condition
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Multi-Component
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Gene ID(s)
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3440
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UniProt ID(s)
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P01563
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Schedule B Code
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3822.19.0000
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Product Description
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Storage
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Quantity per Kit
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S-PLEX Human IFN-α2a Antibody Set
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2-8 °C
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1 each
(1 plate size)
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Human IFN-α2a Calibrator
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2-8 °C
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1 vial
(1 vial)
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S-PLEX Coating Reagent C1
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≤-70 °C
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1 vial
(300 μL)
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S-PLEX Detect D2 (200X)
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≤-70 °C
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1 each
(50 μL)
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S-PLEX Detect D1 (4X)
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≤-70 °C
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1 each
(1.7 mL)
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S-PLEX 96-Well SECTOR Plate
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2-8 °C
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1 each
(1 plate)
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2-8 °C
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1 bottle
(50 mL)
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Diluent Assembly 23 (includes Diluent 43 – 10 mL, and Diluent 3 – 8 mL)
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≤-10 °C
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1 each
(1 each)
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S-PLEX Enhance E1 (4X)
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≤-10 °C
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1 each
(1.7 mL)
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S-PLEX Enhance E2
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≤-10 °C
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1 vial
(1.7 mL)
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S-PLEX Enhance E3 (200X)
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≤-70 °C
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1 each
(50 μL)
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RT
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1 bottle
(18 mL)
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≤-10 °C
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1 vial
(500 μL)
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Product Description
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Storage
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Quantity per Kit
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S-PLEX Human IFN-α2a Antibody Set
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2-8 °C
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1 each
(5 plate size)
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Human IFN-α2a Calibrator
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2-8 °C
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5 vial
(1 vial)
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S-PLEX Coating Reagent C1
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≤-70 °C
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1 vial
(300 μL)
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S-PLEX Detect D2 (200X)
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≤-70 °C
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5 each
(50 μL)
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S-PLEX Detect D1 (4X)
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≤-70 °C
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5 each
(1.7 mL)
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S-PLEX 96-Well SECTOR Plate
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2-8 °C
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5 each
(1 plate)
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2-8 °C
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1 bottle
(50 mL)
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Diluent Assembly 24 (includes Diluent 43 – 50 mL, and Diluent 3 – 40 mL)
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≤-10 °C
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1 each
(1 each)
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S-PLEX Enhance E1 (4X)
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≤-10 °C
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5 each
(1.7 mL)
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S-PLEX Enhance E2
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≤-10 °C
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5 vial
(1.7 mL)
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S-PLEX Enhance E3 (200X)
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≤-70 °C
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5 each
(50 μL)
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RT
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1 bottle
(90 mL)
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≤-10 °C
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1 vial
(500 μL)
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Product Description
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Storage
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Quantity per Kit
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S-PLEX Human IFN-α2a Antibody Set
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2-8 °C
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5 each
(5 plate size)
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Human IFN-α2a Calibrator
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2-8 °C
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25 vial
(1 vial)
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S-PLEX Coating Reagent C1
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≤-70 °C
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5 vial
(300 μL)
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S-PLEX Detect D2 (200X)
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≤-70 °C
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25 each
(50 μL)
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S-PLEX Detect D1 (4X)
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≤-70 °C
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25 each
(1.7 mL)
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S-PLEX 96-Well SECTOR Plate
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2-8 °C
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25 each
(1 plate)
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2-8 °C
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5 bottle
(50 mL)
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Diluent Assembly 24 (includes Diluent 43 – 50 mL, and Diluent 3 – 40 mL)
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≤-10 °C
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5 each
(1 each)
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S-PLEX Enhance E1 (4X)
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≤-10 °C
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25 each
(1.7 mL)
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S-PLEX Enhance E2
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≤-10 °C
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25 vial
(1.7 mL)
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S-PLEX Enhance E3 (200X)
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≤-70 °C
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25 each
(50 μL)
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RT
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5 bottle
(90 mL)
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≤-10 °C
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5 vial
(500 μL)
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Product Inserts
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Certificate of analysis
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SDS
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Title
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Journal
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Year
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Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses
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Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics
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Endogenous intrahepatic IFNs and association with IFN-free HCV treatment outcome.
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