Click image to enlarge
STAT 5a and 5b are members of the STAT family of transcription factors, part of the JAK/STAT signal transduction pathway, and approximately 90% identical at the amino acid level. When inactive, STATs are cytoplasmic. Upon ligand binding and activation of a cytokine receptor, the receptor binds to a member of the JAK family. The receptor is phosphorylated and recruits a member of the STAT family that is then phosphorylated. It then dimerizes and is transported into the nucleus, where it can act as a transcription factor. In all, there are about 38 cytokines that signal through their cytokine receptors and through the JAK/STAT pathway to cause downstream transcriptional effects. Interestingly, these effects seem to be cell-type specific and JAK/STAT specific. To name a few, STAT5s respond to cellular stimulation by IL-2, IL-3, IL-5, IL-7, GM-CSF, IFN-g, insulin, erythropoietin, growth hormone, and prolactin.
STAT5a and 5b are involved in different types of cancer (including breast and prostate), inflammatory responses, and allergic reactions. STAT5s also prevent apoptosis in certain cell types and diseases based on their role as transcription factors and the genes they regulate. Due to the transcriptional control provided by the STAT family of proteins, their role in intracellular signaling, and their significance in diseases such as cancer and inflammation, this important signaling pathway is the subject of a number of basic research and pharmacological studies. This kit provides the assay-specific components for the quantitative determination of endogenous STAT 5a and STAT 5b in human, mouse, and rat cell lysates.
-
-
Specifications
-
Kit Contents
-
Documentation
-
Application(s)
|
|
Phosphoproteins
,
Intracellular Signaling
|
|
Analyte(s)
|
|
STAT5a/b
|
|
Species
|
|
Human, Mouse, Rat
|
|
Instrument(s)
|
|
SECTOR Imager 2400
,
MESO SECTOR S 600MM
,
SECTOR Imager 6000
,
MESO QuickPlex SQ 120MM
,
MESO QuickPlex SQ 120
,
MESO SECTOR S 600
|
|
Plate Type
|
|
96-well
|
|
Capture Antibody
|
|
Phospho-STAT 5a,b: Mouse MonoclonalTotal STAT 5a: Mouse MonoclonalTotal STAT 5b: Mouse Monoclonal
|
|
Detection Antibody
|
|
STAT 5a: Mouse MonoclonalSTAT 5b: Mouse Monoclonal
|
|
Usage Statement
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
|
Kit Model
|
|
Whole Cell Lysate
|
|
Storage Statement(s)
|
|
Please refer to the product insert for the storage conditions of individual kit components.
|
|
Storage Condition
|
|
Multi-Component
|
|
Schedule B Code
|
|
3822.19.0000
|
|
Product Description
|
Storage
|
Quantity per Kit
|
Phosphatase Inhibitor I 0.1 mL
|
2-8 °C
|
1 each (0.1 mL)
|
|
Phosphatase Inhibitor II 0.1 mL
|
2-8 °C
|
1 each (1 each)
|
|
Protease Inhibitor Solution 0.1 mL
|
2-8 °C
|
1 each (1 each)
|
|
SULFO-TAG Anti-Total STAT5a,b Antibody
|
2-8 °C
|
1 vial (75 μL)
|
|
Phospho(Tyr694)/Total STAT5a,b Plate
|
2-8 °C
|
1 each (1 each)
|
|
|
2-8 °C
|
1 bottle (50 mL)
|
|
|
2-8 °C
|
1 bottle (200 mL)
|
|
|
RT
|
1 bottle (50 mL)
|
|
|
RT
|
1 bottle (15 g)
|
|
|
≤-10 °C
|
1 vial (0.2 mL)
|
|
MSD Blocker D-R, 0.05 mL
|
≤-10 °C
|
1 vial (1 vial)
|
|
Product Description
|
Storage
|
Quantity per Kit
|
Phosphatase Inhibitor I 0.5mL
|
2-8 °C
|
1 each (1 each)
|
|
Phosphatase Inhibitor II 0.5mL
|
2-8 °C
|
1 each (1 each)
|
|
Protease Inhibitor Solution 0.5mL
|
2-8 °C
|
1 each (1 each)
|
|
SULFO-TAG Anti-Total STAT5a,b Antibody
|
2-8 °C
|
1 vial (375 μL)
|
|
Phospho(Tyr694)/Total STAT5a,b Plate
|
2-8 °C
|
5 each (1 each)
|
|
|
2-8 °C
|
1 bottle (50 mL)
|
|
|
2-8 °C
|
1 bottle (200 mL)
|
|
|
RT
|
1 bottle (50 mL)
|
|
|
RT
|
1 bottle (15 g)
|
|
|
≤-10 °C
|
1 vial (0.2 mL)
|
|
Product Description
|
Storage
|
Quantity per Kit
|
Phosphatase Inhibitor I 2.0mL
|
2-8 °C
|
1 each (1 each)
|
|
Phosphatase Inhibitor II 2.0mL
|
2-8 °C
|
1 each (1 each)
|
|
Protease Inhibitor Solution 2.0mL
|
2-8 °C
|
1 each (1 each)
|
|
SULFO-TAG Anti-Total STAT5a,b Antibody
|
2-8 °C
|
4 vial (375 μL)
|
|
Phospho(Tyr694)/Total STAT5a,b Plate
|
2-8 °C
|
20 each (1 each)
|
|
|
2-8 °C
|
1 bottle (200 mL)
|
|
|
2-8 °C
|
1 bottle (1000 mL)
|
|
|
RT
|
1 bottle (200 mL)
|
|
|
RT
|
1 bottle (15 g)
|
|
|
≤-10 °C
|
4 vial (0.2 mL)
|
|
Product Inserts
|
|
|
Datasheets
|
|
|
Certificate of analysis
|
|
|
SDS
|
|
|
Order Details
Catalog No
Size
Price in USD
Qty
Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters