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Phospho-STAT Panel Kit

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Please note: There is a new version of this kit that uses improved antibodies, a 10-spot plate, and MSD GOLD Read Buffer B. Click here to be directed to the new version. This legacy kit will be available until December 31, 2026 to allow time to transition to the new kit. Contact your account manager to discuss your transition plan, or Technical Support if further assistance is needed.

Specifications
Kit Contents
Documentation
References

Application(s)		Phosphoproteins , Phosphoproteins , Intracellular Signaling
Analyte(s)		STAT3, STAT4, STAT5a,b (total)
Species		Human, Mouse, Rat
Plate Type		96-well
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K15202D-1

Product Description	Storage	Quantity per Kit
Phosphatase Inhibitor I 0.1 mL	2-8 °C	1 each (0.1 mL)

Phosphatase Inhibitor II 0.1 mL	2-8 °C	1 each (1 each)

Protease Inhibitor Solution 0.1 mL	2-8 °C	1 each (1 each)

SULFO-TAG Anti-Total STAT4 Antibody	2-8 °C	1 vial (75 μL)

SULFO-TAG Anti-Phospho-STAT3 (Tyr705) Antibody	2-8 °C	1 vial (1 vial)

SULFO-TAG Anti-Total STAT5a,b Antibody	2-8 °C	1 vial (75 μL)

Phospho-STAT Panel Plate	2-8 °C	1 each (1 each)

MSD Tris Lysis Buffer	2-8 °C	1 bottle (50 mL)

MSD Tris Wash Buffer (10x)	2-8 °C	1 bottle (200 mL)

Read Buffer T 4X	RT	1 bottle (50 mL)

MSD Blocker A	RT	1 bottle (15 g)

MSD Blocker D-M, 0.2 mL	≤-10 °C	1 vial (0.2 mL)

MSD Blocker D-R, 0.05 mL	≤-10 °C	1 vial (1 vial)

Kit Contents for Catalog No. K15202D-2

Product Description	Storage	Quantity per Kit
Phosphatase Inhibitor I 0.5mL	2-8 °C	1 each (1 each)

Phosphatase Inhibitor II 0.5mL	2-8 °C	1 each (1 each)

Protease Inhibitor Solution 0.5mL	2-8 °C	1 each (1 each)

SULFO-TAG Anti-Total STAT4 Antibody	2-8 °C	1 vial (375 μL)

SULFO-TAG Anti-Phospho-STAT3 (Tyr705) Antibody	2-8 °C	1 vial

SULFO-TAG Anti-Total STAT5a,b Antibody	2-8 °C	1 vial (375 μL)

Phospho-STAT Panel Plate	2-8 °C	5 each (1 each)

MSD Tris Lysis Buffer	2-8 °C	1 bottle (50 mL)

MSD Tris Wash Buffer (10x)	2-8 °C	1 bottle (200 mL)

Read Buffer T 4X	RT	1 bottle (50 mL)

MSD Blocker A	RT	1 bottle (15 g)

MSD Blocker D-M, 1.8 mL	≤-10 °C	1 vial (1.8 mL)

MSD Blocker D-R	≤-10 °C	1 vial (1 mL)

Kit Contents for Catalog No. K15202D-4

Product Description	Storage	Quantity per Kit
Phosphatase Inhibitor I 0.5mL	2-8 °C	5 each (1 each)

Phosphatase Inhibitor II 0.5mL	2-8 °C	5 each (1 each)

Protease Inhibitor Solution 0.5mL	2-8 °C	5 each (1 each)

SULFO-TAG Anti-Total STAT4 Antibody	2-8 °C	5 vial (375 μL)

SULFO-TAG Anti-Phospho-STAT3 (Tyr705) Antibody	2-8 °C	5 vial

SULFO-TAG Anti-Total STAT5a,b Antibody	2-8 °C	5 vial (375 μL)

Phospho-STAT Panel Plate	2-8 °C	25 each (1 each)

MSD Tris Lysis Buffer	2-8 °C	5 bottle (50 mL)

MSD Tris Wash Buffer (10x)	2-8 °C	5 bottle (200 mL)

Read Buffer T 4X	RT	5 bottle (50 mL)

MSD Blocker A	RT	5 bottle (15 g)

MSD Blocker D-M, 1.8 mL	≤-10 °C	5 vial (1.8 mL)

MSD Blocker D-R	≤-10 °C	5 vial (1 mL)

Order Details

K15202D

Select your instrument:

S 600

SQ 120

Q 60

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		STAT
Product Highlights		STAT Product Highlights
SDS		Blocker A Phosphatase Inhibitor I Protease Inhibitor Solution Read Buffer T (4x), Read Buffer S (4x) and Read Buffer S (1x) Tris Lysis Buffer Tris Wash Buffer (10x)
Scientific Posters		High Throughput Assay to Screen for Inhibitors of the Proto-Oncogene Stat3

Title	Journal	Year
A randomized, placebo-controlled study of the pharmacokinetics, pharmacodynamics, and tolerability of the oral JAK2 inhibitor fedratinib (SAR302503) in healthy volunteers. Zhang M, Xu CR, Shamiyeh E, Liu F, Yin J, von Moltke LL, Smith WB.	J Clin Pharmacol.	2013
Characterization of a high-affinity human antibody with a disulfide bridge in the third complementarity-determining region of the heavy chain. Almagro JC, Raghunathan G, Beil E, Janecki DJ, Chen Q, Dinh T, Lacombe A, Connor J, Ware M, Kim PH, Swanson RV, Fransson J.	J Mol Recognit.	2012
Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233. Gurley SN, Abidi AH, Allison P, Guan P, Duntsch C, Robertson JH, Kosanke SD, Keir ST, Bigner DD, Elberger AJ, Moore BM 2nd.	J Neurooncol.	2012
Development and Utilization of Activated STAT3 Detection Assays for Screening a Library of Secreted Proteins. Fursov, N., Gates, I.V., Panavas, T., Giles-Komar, J., Powers, G.	Assay and Drug Dev Technol.	2011
Cooperative signaling through the signal transducer and activator of transcription 3 and nuclear factor-{kappa}B pathways in subtypes of diffuse large B-cell lymphoma. Lam, L.T., Wright, G., Davis, R.E., Lenz, G., Farinha, P., Dang, L., Chan, J.W., Rosenwald, A., Gascoyne, R.D., Staudt, L.M.	Blood	2008

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Phospho-STAT Panel Kit

Kit Contents for Catalog No. K15202D-1

Kit Contents for Catalog No. K15202D-2

Kit Contents for Catalog No. K15202D-4

Order Details

K15202D

S 600

SQ 120

Q 60

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