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IGF (Insulin like Growth Factor) signaling is mediated by the ligands IGF1 and IGF2, and the receptors IGF-1R (Insulin like Growth Factor-1 Receptor), IGF-2R, and IR (Insulin Receptor). Additionally, downstream signaling from IGF-1R/IR through the PI3K signaling pathway is accomplished due to the adaptor protein IRS-1 (Insulin Receptor Substrate-1) binding to the phosphorylated and activated IGF-1R or IR. IR and IGF-1R signaling play a key role in normal development, growth, metabolism, and cellular homeostasis as well as being implicated in such disease processes as growth abnormalities, metabolic processes, and different types of cancer. Pharmacological disruption of IGF-1R/IR signaling is a fertile area of research, but due a critical role in normal cellular metabolism and growth, the ability to identify selective therapeutics targeting abnormally signaling cells would be greatly advantageous. This kit provides the assay-specific components for the quantitative determination of phospho-IGF-1R, phospho-IR, and phospho-IRS-1 in human whole cell lysates.
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Specifications
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Kit Contents
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Documentation
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References
Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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IGF-1R, IR, IRS-1
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Species
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Human
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
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MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
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MESO SECTOR S 600
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Plate Type
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96-well
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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2-8 °C
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Schedule B Code
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3822.19.0000
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.1 mL
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2-8 °C
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1 each (0.1 mL)
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Phosphatase Inhibitor II 0.1 mL
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2-8 °C
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1 each (1 each)
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Protease Inhibitor Solution 0.1 mL
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2-8 °C
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1 each (1 each)
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SULFO-TAG Anti-Phospho-Tyrosine Antibody
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2-8 °C
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1 vial (75 μL)
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Insulin Signaling Panel Plate
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2-8 °C
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1 each (1 each)
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2-8 °C
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1 bottle (50 mL)
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2-8 °C
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1 bottle (200 mL)
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RT
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1 bottle (50 mL)
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RT
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1 bottle (15 g)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.5mL
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2-8 °C
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1 each (1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8 °C
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1 each (1 each)
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Protease Inhibitor Solution 0.5mL
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2-8 °C
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1 each (1 each)
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SULFO-TAG Anti-Phospho-Tyrosine Antibody
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2-8 °C
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1 vial (375 μL)
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Insulin Signaling Panel Plate
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2-8 °C
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5 each (1 each)
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2-8 °C
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1 bottle (50 mL)
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2-8 °C
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1 bottle (200 mL)
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RT
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1 bottle (50 mL)
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RT
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1 bottle (15 g)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 2.0mL
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2-8 °C
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1 each (1 each)
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Phosphatase Inhibitor II 2.0mL
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2-8 °C
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1 each (1 each)
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Protease Inhibitor Solution 2.0mL
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2-8 °C
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1 each (1 each)
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SULFO-TAG Anti-Phospho-Tyrosine Antibody
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2-8 °C
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4 vial (375 μL)
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Insulin Signaling Panel Plate
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2-8 °C
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20 each (1 each)
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2-8 °C
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1 bottle (200 mL)
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2-8 °C
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1 bottle (1000 mL)
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RT
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1 bottle (200 mL)
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RT
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1 bottle (15 g)
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Product Inserts
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Datasheets
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Certificate of analysis
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SDS
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Title
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Journal
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Year
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Targeting IGF-IR with ganitumab inhibits tumorigenesis and increases durability of response to androgen-deprivation therapy in VCaP prostate cancer xenografts.
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Dependence of Wilms tumor cells on signaling through insulin-like growth factor 1 in an orthotopic xenograft model targetable by specific receptor inhibition.
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Neutrophils mediate insulin resistance in mice fed a high-fat diet through secreted elastase.
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Dual IGF-I/II-Neutralizing Antibody MEDI-573 Potently Inhibits IGF Signaling and Tumor Growth.
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Efficacy of Ganitumab (AMG 479), Alone and in Combination With Rapamycin, in Ewing's and Osteogenic Sarcoma Models.
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