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Cleaved/Total Caspase-3 Whole Cell Lysate Kit

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Caspase-3 plays a key role in the regulation of apoptosis. Initially synthesized as a precursor protein, it is activated by proteolytic cleavage of the N-terminus. The Cleaved/Total Caspase-3 Whole Cell Lysate Kit provides assay specific components for the quantitative determination of the p20 and p17 cleavage (ASP175) products of caspase-3 and total caspase-3 in human whole cell lysates.

Specifications
Kit Contents
Documentation
References

Application(s)		Phosphoproteins , Intracellular Signaling
Analyte(s)		Caspase-3
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO SECTOR S 600MM , SECTOR Imager 6000 , MESO QuickPlex SQ 120MM , MESO QuickPlex SQ 120 , MESO SECTOR S 600
Plate Type		96-well
Capture Antibody		Cleaved Caspase-3: Rabbit Polyclonal Caspase-3: Goat Polyclonal
Detection Antibody		Cleaved Caspase-3: Goat Polyclonal Caspase-3: Goat Polyclonal
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Kit Model		Whole Cell Lysate
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		2-8 °C
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K15140D-1

Product Description	Storage	Quantity per Kit
Protease Inhibitor Solution 0.1 mL	2-8 °C	1 each (1 each)

SULFO-TAG Anti-Caspase-3 Antibody	2-8 °C	1 vial (75 μL)

Cleaved/Total Caspase-3 Plate	2-8 °C	1 each (1 each)

MSD Tris Lysis Buffer	2-8 °C	1 bottle (50 mL)

MSD Tris Wash Buffer (10x)	2-8 °C	1 bottle (200 mL)

Read Buffer T 4X	RT	1 bottle (50 mL)

MSD Blocker A	RT	1 bottle (15 g)

Kit Contents for Catalog No. K15140D-2

Product Description	Storage	Quantity per Kit
Protease Inhibitor Solution 0.5mL	2-8 °C	1 each (1 each)

SULFO-TAG Anti-Caspase-3 Antibody	2-8 °C	1 vial (375 μL)

Cleaved/Total Caspase-3 Plate	2-8 °C	5 each (1 each)

MSD Tris Lysis Buffer	2-8 °C	1 bottle (50 mL)

MSD Tris Wash Buffer (10x)	2-8 °C	1 bottle (200 mL)

Read Buffer T 4X	RT	1 bottle (50 mL)

MSD Blocker A	RT	1 bottle (15 g)

Kit Contents for Catalog No. K15140D-3

Product Description	Storage	Quantity per Kit
Protease Inhibitor Solution 2.0mL	2-8 °C	1 each (1 each)

SULFO-TAG Anti-Caspase-3 Antibody	2-8 °C	4 vial (375 μL)

Cleaved/Total Caspase-3 Plate	2-8 °C	20 each (1 each)

Tris Lysis Buffer	2-8 °C	1 bottle (200 mL)

Tris Wash Buffer	2-8 °C	1 bottle (1000 mL)

Read Buffer T 4X	RT	1 bottle (200 mL)

MSD Blocker A	RT	1 bottle (15 g)

Order Details

Catalog No

Size

Price in USD

Qty

K15140D-1

1 plate

$1,035.00

Usually ships in 1-3 days

K15140D-2

5 plates

$4,252.00

Usually ships in 1-3 days

K15140D-3

20 plates

$14,430.00

Usually ships in 1-3 days

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		Cleaved Total Caspase3 WCL
Datasheets		DS Cleaved Total Caspase3
SDS		Blocker A Protease Inhibitor Solution Read Buffer T (4x), Read Buffer S (4x) and Read Buffer S (1x) Tris Lysis Buffer Tris Wash Buffer (10x)

Title	Journal	Year
Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233. Gurley SN, Abidi AH, Allison P, Guan P, Duntsch C, Robertson JH, Kosanke SD, Keir ST, Bigner DD, Elberger AJ, Moore BM 2nd.	J Neurooncol.	2012
The CEA/CD3-Bispecific Antibody MEDI-565 (MT111) Binds a Nonlinear Epitope in the Full-Length but Not a Short Splice Variant of CEA. Peng L, Oberst MD, Huang J, Brohawn P, Morehouse C, Lekstrom K, Baeuerle PA, Wu H, Yao Y, Coats SR, Dall'acqua W, Damschroder M, Hammond SA.	PLoS One.	2012
Optimization of Circulating Biomarkers of Obatoclax-Induced Cell Death in Patients with Small Cell Lung Cancer. Dean, E.J., Cummings, J., Roulston, A., Berger, M., Ranson, M., Blackhall, F., Dive, C.	Neoplasia.	2011
A novel small-molecule inhibitor of protein kinase D blocks pancreatic cancer growth in vitro and in vivo. Harikumar, K.B., Kunnumakkara, A.B., Ochi, N., Tong, Z., Deorukhkar, A., Sung, B., Kelland, L., Jamieson, S., Sutherland, R., Raynham, T., Charles, M., Bagherazadeh, A., Foxton, C., Boakes, A., Farooq, M., Maru, D., Diagaradjane, P., Matsuo, Y., Sinnett-Smith, J., Gelovani, J., Krishnan, S., Aggarwal, B.B., Rozengurt, E., Ireson, C.R., Guha, S.	Mol Cancer Ther.	2010
A small molecule inhibitor of XIAP induces apoptosis and synergizes with vinorelbine and cisplatin in NSCLC. Dean, E.J., Ward, T., Pinilla, C., Houghten, R., Welsh, K., Makin, G., Ranson, M., Dive, C.	Br J Cancer.	2010
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Cleaved/Total Caspase-3 Whole Cell Lysate Kit

Kit Contents for Catalog No. K15140D-1

Kit Contents for Catalog No. K15140D-2

Kit Contents for Catalog No. K15140D-3

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