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Calibrator 2 is a lyophilized blend of nine recombinant human proteins: Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, and TARC. It was developed for use as an assay calibrator for MSD U-PLEX human and non-human primate assays that measure any of the included proteins. The utility of this product has not been evaluated in other applications. Individual analyte concentrations are provided in the lot-specific certificates of analysis. All analytes in Calibrator 2 were expressed in E. coli. The instructions on using Calibrator 2 in U-PLEX assays are provided in U-PLEX Biomarker Group 1 Human Product Inserts and U-PLEX Biomarker Group 1 NHP Product Inserts.