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The Apoptosis Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of total p53, phospho-p53 (Ser15), PARP (cleaved), and Caspase-3 (cleaved) in human whole cell lysates. Caspase-3 is initially synthesized as a precursor protein but is activated by proteolytic cleavage of the N-terminus to produce the p20 and p17 cleavage (ASP175) products measured in this kit. PARP becomes inactive following cleavage (ASP214), however, the small cleavage product does retain its DNA-binding capabilities, blocking access to damaged DNA and inhibiting further DNA repair.
Please note: MSD has transitioned this product to a status of “made-to-order” with a minimum order size of 25 plates. For an order of the 5-plate pack size, the minimum quantity is 5 packs (25 plates). The 1-plate and 20-plate pack sizes have been discontinued.
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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Caspase-3, p53, PARP
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Species
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Human
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
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MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Cleaved PARP: Rabbit Polyclonal
Cleaved Caspase-3: Rabbit Polyclonal
Phospho-p53: Mouse Monoclonal
p53: Mouse Monoclonal
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Detection Antibody
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Cleaved PARP: Rabbit Polyclonal
Cleaved Caspase-3: Goat Polyclonal
Phospho-p53: Goat Polyclonal
p53: Goat Polyclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Schedule B Code
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3822.19.0000
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.5mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.5mL
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2-8 °C
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1 each
(1 each)
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SULFO-TAG Anti-PARP Antibody
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2-8 °C
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1 vial
(375 μL)
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SULFO-TAG Anti-Caspase-3 Antibody
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2-8 °C
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1 vial
(375 μL)
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SULFO-TAG Anti-p53 (1b, apoptosis) Antibody
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2-8 °C
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1 vial
(375 μL)
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Apoptosis Panel Plate
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2-8 °C
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5 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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RT
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1 bottle
(1 g)
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≤-10 °C
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1 vial
(0.2 mL)
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Product Inserts
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Datasheets
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Certificate of analysis
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SDS
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Title
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Journal
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Year
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Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233.
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The CEA/CD3-Bispecific Antibody MEDI-565 (MT111) Binds a Nonlinear Epitope in the Full-Length but Not a Short Splice Variant of CEA.
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Optimization of Circulating Biomarkers of Obatoclax-Induced Cell Death in Patients with Small Cell Lung Cancer.
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A novel small-molecule inhibitor of protein kinase D blocks pancreatic cancer growth in vitro and in vivo.
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A small molecule inhibitor of XIAP induces apoptosis and synergizes with vinorelbine and cisplatin in NSCLC.
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View All
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Order Details
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