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The Akt Signaling (Total Protein) Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of total Akt, p70S6K, and GSK-3β in human, mouse, and rat whole cell lysates.
Please note: MSD has transitioned this product to a status of “made-to-order” with a minimum order size of 25 plates. For an order of the 5-plate pack size, the minimum quantity is 5 packs (25 plates). The 1-plate and 20-plate pack sizes have been discontinued.
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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Akt, GSK-3β, p70S6K
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Species
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Human, Mouse, Rat
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
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MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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p70S6K: Mouse Monoclonal
GSK-3β: Rat Monoclonal
Akt: Mouse Monoclonal
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Detection Antibody
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p70S6K: Rabbit Polyclonal
GSK-3β: Mouse Monoclonal
Akt: Mouse Monoclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Schedule B Code
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3822.19.0000
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Product Description
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Storage
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Quantity per Kit
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SULFO-TAG Anti-p70S6K Antibody
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2-8 °C
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1 vial
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SULFO-TAG Anti-GSK-3β Antibody
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2-8 °C
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1 vial
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SULFO-TAG Anti-Akt Antibody
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2-8 °C
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1 vial
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Phosphatase Inhibitor I 0.5mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.5mL
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2-8 °C
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1 each
(1 each)
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Akt Signaling (Total Protein) Panel Plate
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2-8 °C
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5 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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1 vial
(0.2 mL)
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Product Inserts
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Datasheets
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SDS
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Title
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Journal
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Year
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Discovery of diverse and functional antibodies from large human repertoire antibody libraries.
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Dual blockade of the PI3K/AKT/mTOR (AZD8055) and RAS/MEK/ERK (AZD6244) pathways synergistically inhibits rhabdomyosarcoma cell growth in vitro and in vivo.
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Functional Consequences of the Macrophage Stimulating Protein 689C Inflammatory Bowel Disease Risk Allele.
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Multimodal microvascular imaging reveals that selective inhibition of class I PI3K is sufficient to induce an antivascular response.
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S49076 is a novel kinase inhibitor of MET, AXL and FGFR with strong preclinical activity alone and in association with bevacizumab.
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View All
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Order Details
Catalog No
Size
Price in USD
Qty
Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters