While the Aβ42 assay was validated with a washed assay, a homogenous protocol may be used with similar performance and sensitivity. Samples were not validated with the homogenous protocol. The homogeneous protocol is provided in the Abeta 3-Plex kits. The basic protocol is as follows:

• Block plate with 1% Blocker A for 1 hour and wash.
• Add 25 µl of detection antibody. Add 25 µl of sample. Incubate for 2 hours and wash.
• Add 150 µl of Read Buffer T (2X) and read.