Scientific Frequently Asked Question

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FAQ	Category
What standards are used to calibrate the assays? The assays are developed using positive and negative control Whole Cell Lysate S...	Assays
What type of payments does MSD accept? MSD accepts payments via purchase order. You may enter this information during t...	Ordering
What volume of lysate should I use? A minimum of 25 µL should be placed in each well to ensure adequate covera...	Assays
When I add more lysate to increase assay signals, why do the signals continue to decrease? High concentration of proteins in the sample can inhibit antibody binding, espec...
Where can I find general information about MSD assay development and plate spotting? The MSD Immunoblot and General Notes technical presentation includes detailed in...	Assays
Where can I find information about immunogenicity assay development? This product insert provides detailed information about using MSD Bridging ...	Assays
Where can I find the calibrator/standard curve concentrations for V-PLEX assays? A certificate of analysis (COA) is provided with each V-PLEX kit lot including t...	Assays
Where do I enter our tax exempt status? Tax exempt status and/or tax IDs are not required during checkout. We manage thi...	Ordering
Which diluent should I use for CSF samples? Diluent 35 is the best option as it closely mimics the sample matrix (it is esse...	Assays
Who do I contact for MSD’s Custom Conjugation Services? Please contact the MSD Customer Service Department at customerservice@mesos...
Why are my unknown sample values being reported as N/A when the signal is within standard curve range? As long as standards are assigned first, controls and unknowns will automaticall...	Software
Why are the lot numbers for my controls different from the ones shown in the certificate of analysis (COA) for my V-PLEX kit? The MSD control packs are not specific to a particular V-PLEX kit lot. Thi...	Assays
Why are the singleplex validated assays on a 4-spot plate? The performance of a small-spot plate and 4-spot plate are equivalent. We are wo...	Assays
Why did I get a message that my data has been quarantined? How do I avoid this message? Your data may be quarantined if the barcode reader fails to read the plate barco...	Software
Why do I need to dilute my CSF samples for Aß42, I usually use undiluted CSF? Other kits also use undiluted samples. We determined that a 1:8 dilution of CSF provided the best combination of matrix...	Assays
Why do I need to register? Registration is required in order for you to see customer-specific pricing, plac...	Ordering
Why do I not see the same dynamic range as I see with cytokine assays? Non-specific binding of antibodies, especially phospho-specific antibodies, limi...	Assays
Why do only some of the V-PLEX assays have NIBSC/WHO reference information? NIBSC/WHO standards are not available for all analytes. Results are included in ...	Assays
Why do the calibrator curve concentrations change from one lot to the next for V-PLEX assays? Calibrators are re-evaluated with each new V-PLEX production lot to provide opti...	Assays
Why do the concentrations of the highest calibrators in the certificate of analysis (COA) for validated kits vary between kit lots? As part of the kit lot verification process, we compare the calibrator for the n...	Assays
Why is the Human Aß42 Kit LLOD given as a range? Should I expect a mid-range LLOD value? The LLOD was calculated across many runs. Since the LLOD is computed as 2.5 stan...	Assays
Why was Wash Buffer not included in my V-PLEX assay kit? Wash Buffer is only included with the “V-PLEX Plus” version of the k...	Assays Reagents Diluents Buffers
Will the new tau calibrator be incorporated into the other Tau assays? MSD is currently working on new calibrators for our existing Total Tau and phosp...	Assays
Will the validated Alzheimer's kits be provided with controls? MSD sells quality controls as a separate package (Neurodegeneration Control Pack...	Assays
Will the validated Human Aß42 and Human Total Tau kits work with other sample types? The assay was validated with human CSF. The kits have been run with tissue cultu...

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