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U-PLEX Proinflam Combo 2 (human)

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U-PLEX Combos offer popular panels of biomarkers that have been grouped together for convenient ordering. They have been tested for multiplex compatibility and are fulfilled as a kit. The U-PLEX Proinflam Combo 2 (human) contains assays for 9 proinflammatory biomarkers (Table 1). The assays can be used for quantitative measurements in the sample types listed in the "Specifications" tab below, and offer detection limits (LLODs) in the sub- to low pg/mL range.

Table 1. List of analytes and typical detection limits

Analyte	LLOD - ULOD	units
GM-CSF	0.12 - 9,400	pg/mL
IFN-γ	1.7 - 17,000	pg/mL
IL-1β	0.15 - 3,800	pg/mL
IL-2	0.70 - 1,900	pg/mL
IL-4	0.076 - 2,100	pg/mL
IL-6	0.33 - 2,000	pg/mL
IL-8	0.15 - 2,200	pg/mL
IL-10	0.14 - 3,700	pg/mL
IL-12p70	0.69 - 5,300	pg/mL

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO SECTOR S 600MM , SECTOR Imager 6000 , MESO QuickPlex SQ 120MM , MESO QuickPlex SQ 120 , MESO SECTOR S 600
U-PLEX Group Compatibility		Biomarker Group 1
Plate Type		96-well
Sample Type		Serum, Plasma, Cell Culture Supernatant
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component
Schedule B Code		3822.19.0000

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 9-Assay (300 µL)	2-8 °C	1 each (1 vial)

U-PLEX Human IFN-γ Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human IL-1β Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human IL-2 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human IL-4 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human IL-6 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human IL-8 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human IL-10 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human IL-12p70 Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

U-PLEX Human GM-CSF Antibody Set (1 Plate Size)	2-8 °C	1 each (1 plate size)

Calibrator 1	2-8 °C	1 vial (1 vial)

U-PLEX 9-Assay, 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)

Diluent 3	≤-10 °C	1 bottle (8 mL)

Diluent 57	≤-10 °C	1 bottle (10 mL)

MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 9-Assay (1.8 mL)	2-8 °C	1 each (1 vial)

U-PLEX Human IFN-γ Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human IL-1β Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human IL-2 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human IL-4 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human IL-6 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human IL-8 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human IL-10 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human IL-12p70 Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

U-PLEX Human GM-CSF Antibody Set (5 Plate Size)	2-8 °C	1 each (5 plate size)

Calibrator 1	2-8 °C	5 vial (1 vial)

U-PLEX 9-Assay, 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)

Diluent 3	≤-10 °C	1 bottle (40 mL)

Diluent 57	≤-10 °C	1 bottle (50 mL)

MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

Product Description	Storage	Quantity per Kit
U-PLEX Linker Set, 9-Assay (1.8 mL)	2-8 °C	5 each (1 vial)

U-PLEX Human IFN-γ Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human IL-1β Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human IL-2 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human IL-4 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human IL-6 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human IL-8 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human IL-10 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human IL-12p70 Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

U-PLEX Human GM-CSF Antibody Set (5 Plate Size)	2-8 °C	5 each (5 plate size)

Calibrator 1	2-8 °C	25 vial (1 vial)

U-PLEX 9-Assay, 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

U-PLEX Stop Solution	2-8 °C	5 bottle (40 mL)

Diluent 3	≤-10 °C	5 bottle (40 mL)

Diluent 57	≤-10 °C	5 bottle (50 mL)

MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

Order Details

Catalog No

Size

Price in USD

Qty

K15066K-1

1 plate

$1,898.00

Usually ships in 1-3 days

K15066K-2

5 plates

$8,064.00

Usually ships in 1-3 days

K15066K-4

25 plates

$35,547.00

Usually ships in 1-3 days

Contact Information

Meso Scale Japan 株式会社
電話: 03.6265.7384
〒107-0062
東京都港区南青山 1-1-1 新青山ビル西館１４階 Vistra　Japan内

Product Inserts		U-PLEX Biomarker Group 1 (human) Multiplex Insert
Datasheets		U-PLEX Human GM-CSF U-PLEX Human IFN-γ U-PLEX Human IL-10 U-PLEX Human IL-12p70 U-PLEX Human IL-1β U-PLEX Human IL-2 U-PLEX Human IL-4 View More
Product Highlights		U-PLEX Platform Highlights
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 57, Diluent 58, and Diluent 59 MSD Calibrators and Controls MSD GOLD Read Buffer B U-PLEX Linker Set

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
A phase 1 study of prophylactic anakinra to mitigate ICANS in patients with large B-cell lymphoma Paolo Strati, Andrew Jallouk, Qing Deng, Xubin Li, Lei Feng, Ryan Sun, Sherry Adkins, Swapna Johncy, Taylor Cain, Raphael E. Steiner, Sairah Ahmed, Dai Chihara, Luis E. Fayad, Swaminathan P. Iyer, Sandra Horowitz, Loretta J. Nastoupil, Ranjit Nair, Ahmed Hassan, Taher E. Daoud, Misha Hawkins, Maria A. Rodriguez, Elizabeth J. Shpall, Jeremy L. Ramdial, Partow Kebriaei, David S. Hong, Jason R. Westin, Sattva S. Neelapu, Michael R. Green	Blood Advances	2023
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations Bing Sun, Norina Tang, Michael J. Peluso, Nikita S. Iyer, Leonel Torres, Joanna L. Donatelli, Sadie E. Munter, Christopher C. Nixon, Rachel L. Rutishauser, Isabel Rodriguez-Barraquer, Bryan Greenhouse, John D. Kelly, Jeffrey N. Martin, Steven G. Deeks, Timothy J. Henrich, and Lynn Pulliam	Cells	2021
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
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U-PLEX Proinflam Combo 2 (human)

Kit Contents for Catalog No. K15066K-1

Kit Contents for Catalog No. K15066K-2

Kit Contents for Catalog No. K15066K-4

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