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S-PLEX Human IL-2 Kit

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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IL-2 Assay has the essential components for an ultrasensitive assay that measures IL-2 in a single-analyte format. For detailed instructions on running the IL-2 assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
IL2, TCGF, lymphokine, interleukin 2, capillary leak syndrome, diphtheria

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		IL-2
Species		Human
Instrument(s)		SECTOR Imager 2400 , SECTOR Imager 6000 , MESO QuickPlex SQ 120 , MESO SECTOR S 600 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600MM
Plate Type		96-well
Capture Antibody		Mouse monoclonal
Detection Antibody		Mouse monoclonal
LLOD (Sensitivity)		7.3 fg/mL
Dynamic Range		7.3 - 47,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant
Recombinant standards		Expressed in E. coli
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		3558
UniProt ID(s)		P60568
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151Z2S-1

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-2 Antibody Set	2-8 °C	1 each (1 plate size)

Human IL-2 Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 21 (includes Diluent 2 – 8 mL, and Diluent 3 – 8 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	1 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (18 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151Z2S-2

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-2 Antibody Set	2-8 °C	1 each (5 plate size)

Human IL-2 Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	5 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (90 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151Z2S-4

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-2 Antibody Set	2-8 °C	5 each (5 plate size)

Human IL-2 Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)	≤-10 °C	5 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	25 each (50 μL)

MSD GOLD Read Buffer A	RT	5 bottle (90 mL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Order Details

Catalog No

Size

Price in USD

Qty

K151Z2S-1

1 plate size

$882.00

Usually ships in 1-3 days

K151Z2S-2

5 plate size

$3,735.00

Usually ships in 1-3 days

K151Z2S-4

25 plate size

$15,873.00

Usually ships in 1-3 days

Contact Information

Meso Scale Japan 株式会社
電話: (050) 1706-7074
〒103-0027
東京都中央区日本橋三丁目９番１号日本橋三丁目スクエア 11階 Vistra　Japan内

Product Inserts		S-PLEX IL-2 Kit Product Insert
Certificate of analysis		K00S0181 K00S0164 K00S0146 K00S0047 K00S0024 K00S0001A
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 MSD GOLD Read Buffer A

Title	Journal	Year
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics Alpana Waghmare, Elizabeth M Krantz, Subhasish Baral, Emma Vasquez, Tillie Loeffelholz, E. Lisa Chung, Urvashi Pandey, Jane Kuypers, Elizabeth R Duke, Keith R Jerome, Alexander L Greninger, Daniel B Reeves, Florian Hladik, E Fabian Cardozo-Ojeda, Michael Boeckh, Joshua T Schiffer	TBD	2020
Capturing the systemic immune signature of a norovirus infection: an n-of-1 case study within a clinical trial Antony J. Cutler, Joao Oliveira, Ricardo C. Ferreira, Ben Challis, Neil M. Walker, Sarah Caddy, Jia Lu, Helen E. Stevens, Deborah J. Smyth, Marcin L. Pekalski, Jane Kennet, Kara M.D. Hunter, Ian Goodfellow, Linda S. Wicker, John A. Todd, Frank Waldron-Lynch	Wellcome Open Research	2017
Cytokine biomarkers in tear film for primary open-angle glaucoma Gupta D, Wen JC, Huebner JL, Stinnett S, Kraus VB, Tseng HC, Walsh M.	Clin Ophthalmol.	2017
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S-PLEX Human IL-2 Kit

Kit Contents for Catalog No. K151Z2S-1

Kit Contents for Catalog No. K151Z2S-2

Kit Contents for Catalog No. K151Z2S-4

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