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S-PLEX Human G-CSF Kit

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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human G-CSF Assay has the essential components for an ultrasensitive assay that measures G-CSF in a single-analyte format. For detailed instructions on running the G-CSF assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
CSF3, C17orf33, CSF3OS, GCSF, colony stimulating factor 3, neutropenia

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		G-CSF
Species		Human
Instrument(s)		SECTOR Imager 2400 , SECTOR Imager 6000 , MESO QuickPlex SQ 120 , MESO SECTOR S 600 , MESO QuickPlex SQ 120MM , MESO SECTOR S 600MM
Plate Type		96-well
Capture Antibody		Mouse monoclonal
Detection Antibody		Goat polyclonal
LLOD (Sensitivity)		44 fg/mL
Dynamic Range		44 - 540,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant
Recombinant standards		Expressed in E. coli
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		1440
UniProt ID(s)		P09919
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151K3S-1

Product Description	Storage	Quantity per Kit
S-PLEX Human G-CSF Antibody Set	2-8 °C	1 each (1 plate size)

Human G-CSF Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 23 (includes Diluent 43 – 10 mL, and Diluent 3 – 8 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	1 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (18 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151K3S-2

Product Description	Storage	Quantity per Kit
S-PLEX Human G-CSF Antibody Set	2-8 °C	1 each (5 plate size)

Human G-CSF Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 24 (includes Diluent 43 – 50 mL, and Diluent 3 – 40 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	5 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (90 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151K3S-4

Product Description	Storage	Quantity per Kit
S-PLEX Human G-CSF Antibody Set	2-8 °C	5 each (5 plate size)

Human G-CSF Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent Assembly 24 (includes Diluent 43 – 50 mL, and Diluent 3 – 40 mL)	≤-10 °C	5 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	25 each (50 μL)

MSD GOLD Read Buffer A	RT	5 bottle (90 mL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Order Details

Catalog No

Size

Price in USD

Qty

K151K3S-1

1 plate size

$844.00

Usually ships in 1-3 days

K151K3S-2

5 plate size

$3,574.00

Usually ships in 1-3 days

K151K3S-4

25 plate size

$14,665.00

Usually ships in 1-3 days

Contact Information

Meso Scale Japan 株式会社
電話: 03.6265.7384
〒107-0062
東京都港区南青山 1-1-1 新青山ビル西館１４階 Vistra　Japan内

Product Inserts		S-PLEX G-CSF Kit Product Insert
Certificate of analysis		K00S0187 K00S0120 K00S0113 K00S0061 with Extension memo K00S0021 K00S0013
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 41, Diluent 43 and Diluent 45 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 MSD GOLD Read Buffer A

Title	Journal	Year
Immune response profiling in early rheumatoid arthritis: discovery of a novel interaction of treatment response with viral immunity. Davis JM 3rd, Knutson KL, Strausbauch MA, Green AB, Crowson CS, Therneau TM, Matteson EL, Gabriel SE.	Arthritis Res Ther.	2013
Influence of a polyphenol-enriched protein powder on exercise-induced inflammation and oxidative stress in athletes: a randomized trial using a metabolomics approach. Nieman DC, Gillitt ND, Knab AM, Shanely RA, Pappan KL, Jin F, Lila MA.	PLoS One	2013
A CD4 T cell gene signature for early rheumatoid arthritis implicates interleukin 6-mediated STAT3 signalling, particularly in anti-citrullinated peptide antibody-negative disease. Pratt AG, Swan DC, Richardson S, Wilson G, Hilkens CMU, Young DA, Isaacs JD.	Ann Rheum Dis.	2012
A profile of immune response to herpesvirus is associated with radiographic joint damage in rheumatoid arthritis. Davis JM 3rd, Knutson KL, Skinner JA, Strausbauch MA, Crowson CS, Therneau TM, Wettstein PJ, Matteson EL, Gabriel SE.	Arthritis Res Ther.	2012
Granulocyte colony stimulating factor exacerbates antineutrophil cytoplasmic antibody vasculitis. Freeley SJ, Coughlan AM, Popat RJ, Dunn-Walters DK, Robson MG.	Ann Rheum Dis.	2012
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S-PLEX Human G-CSF Kit

Kit Contents for Catalog No. K151K3S-1

Kit Contents for Catalog No. K151K3S-2

Kit Contents for Catalog No. K151K3S-4

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