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This MAP Kinase Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-JNK (Thr183/Tyr185), phospho-p38 (Thr180/Tyr182), and phospho-ERK-1/2 (Thr/Tyr: 202/204; 185/187) in human, mouse, and rat whole cell lysates.
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Phosphoproteins
,
Intracellular Signaling
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Analyte(s)
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ERK-1/2 (total), JNK, p38 (total)
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Species
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Human, Mouse, Rat
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Instrument(s)
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SECTOR Imager 2400
,
MESO SECTOR S 600MM
,
SECTOR Imager 6000
,
MESO QuickPlex SQ 120MM
,
MESO QuickPlex SQ 120
,
MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Phospho-p38: Mouse Monoclonal
Phospho-ERK1/2: Mouse Monoclonal
Phospho-JNK: Rabbit Polyclonal
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Detection Antibody
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Phospho-p38: Rabbit Polyclonal
Phospho-ERK1/2: Mouse Monoclonal
Phospho-JNK: Mouse Monoclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Schedule B Code
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3822.19.0000
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Product Description
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Storage
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Quantity per Kit
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SULFO-TAG Anti-Phospho-JNK Antibody
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2-8 °C
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1 vial
(1 vial)
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Phosphatase Inhibitor I 0.1 mL
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2-8 °C
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1 each
(0.1 mL)
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Phosphatase Inhibitor II 0.1 mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.1 mL
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2-8 °C
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1 each
(1 each)
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PMSF in DMSO, 0.05 mL
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≤-10 °C
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1 each
(1 each)
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SULFO-TAG Anti-Total Erk1/2 Antibody
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2-8 °C
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1 vial
(75 μL)
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SULFO-TAG Anti-p38 Antibody
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2-8 °C
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1 vial
(75 μL)
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MAP Kinase Panel Plate
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2-8 °C
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1 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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1 vial
(0.2 mL)
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MSD Blocker D-R, 0.05 mL
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≤-10 °C
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1 vial
(1 vial)
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Product Description
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Storage
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Quantity per Kit
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SULFO-TAG Anti-Phospho-JNK Antibody
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2-8 °C
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1 vial
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Phosphatase Inhibitor I 0.5mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.5mL
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2-8 °C
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1 each
(1 each)
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PMSF in DMSO, 0.250 mL
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≤-10 °C
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1 each
(1 each)
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SULFO-TAG Anti-Total Erk1/2 Antibody
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2-8 °C
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1 vial
(375 μL)
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SULFO-TAG Anti-p38 Antibody
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2-8 °C
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1 vial
(375 μL)
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MAP Kinase Panel Plate
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2-8 °C
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5 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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1 vial
(0.2 mL)
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Product Description
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Storage
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Quantity per Kit
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SULFO-TAG Anti-Phospho-JNK Antibody
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2-8 °C
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4 vial
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Phosphatase Inhibitor I 2.0mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 2.0mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 2.0mL
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2-8 °C
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1 each
(1 each)
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PMSF in DMSO, 1.0 mL
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≤-10 °C
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1 each
(1 each)
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SULFO-TAG Anti-Total Erk1/2 Antibody
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2-8 °C
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4 vial
(375 μL)
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SULFO-TAG Anti-p38 Antibody
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2-8 °C
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4 vial
(375 μL)
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MAP Kinase Panel Plate
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2-8 °C
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20 each
(1 each)
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2-8 °C
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1 bottle
(200 mL)
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2-8 °C
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1 bottle
(1000 mL)
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RT
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1 bottle
(200 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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4 vial
(0.2 mL)
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Product Inserts
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Datasheets
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SDS
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Title
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Journal
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Year
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Salidroside and tyrosol from Rhodiola protect H9c2 cells from ischemia/reperfusion-induced apoptosis.
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Chronic treatment with epoxyeicosatrienoic acids modulates insulin signaling and prevents insulin resistance in hepatocytes.
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ERK activation by GM-CSF reduces effectiveness of p38 inhibitor on inhibiting TNFalpha release.
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A novel peptide, colivelin, prevents alcohol-induced apoptosis in fetal brain of C57BL/6 mice: Signaling pathway investigations.
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Characterization of a murine keyhole limpet hemocyanin (KLH)-delayed-type hypersensitivity (DTH) model: role for p38 kinase.
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Meso Scale Japan 株式会社