Click image to enlarge
The ERK-STAT3 Signaling Cascade Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-MEK1/2 (Ser217/221), phospho-ERK-1/2 (Thr/Tyr: 202/204; 185/187), and phospho-STAT3 (Tyr705) in human, mouse, and rat whole cell lysates.
Please note: MSD has transitioned this product to a status of “made-to-order” with a minimum order size of 25 plates. For an order of the 5-plate pack size, the minimum quantity is 5 packs (25 plates). The 1-plate and 20-plate pack sizes have been discontinued.
-
-
Specifications
-
Kit Contents
-
Documentation
-
References
|
Application(s)
|
|
Phosphoproteins
,
Intracellular Signaling
|
|
|
Analyte(s)
|
|
ERK-1/2 (total), MEK 1/2, STAT3
|
|
|
Species
|
|
Human, Mouse, Rat
|
|
|
Instrument(s)
|
|
SECTOR Imager 2400
,
MESO SECTOR S 600MM
,
SECTOR Imager 6000
,
MESO QuickPlex SQ 120MM
,
MESO QuickPlex SQ 120
,
MESO SECTOR S 600
|
|
|
Plate Type
|
|
96-well
|
|
|
Capture Antibody
|
|
ERK1/2: Mouse Monoclonal
MEK1/2: Mouse Monoclonal
STAT3: Mouse Monoclonal
|
|
|
Detection Antibody
|
|
ERK1/2: Mouse Monoclonal
MEK1/2: Rabbit Polyclonal
STAT3: Rabbit Polyclonal
|
|
|
Usage Statement
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
|
|
Kit Model
|
|
Whole Cell Lysate
|
|
|
Storage Statement(s)
|
|
Please refer to the product insert for the storage conditions of individual kit components.
|
|
|
Storage Condition
|
|
Multi-Component
|
|
|
Schedule B Code
|
|
3822.19.0000
|
|
|
Product Description
|
Storage
|
Quantity per Kit
|
|
Phosphatase Inhibitor I 0.5mL
|
2-8 °C
|
1 each
(1 each)
|
|
|
|
Phosphatase Inhibitor II 0.5mL
|
2-8 °C
|
1 each
(1 each)
|
|
|
|
Protease Inhibitor Solution 0.5mL
|
2-8 °C
|
1 each
(1 each)
|
|
|
|
PMSF in DMSO, 0.250 mL
|
≤-10 °C
|
1 each
(1 each)
|
|
|
|
SULFO-TAG Anti-Total Erk1/2 Antibody
|
2-8 °C
|
1 vial
(375 μL)
|
|
|
|
SULFO-TAG Anti-Phospho-MEK1/2 Antibody
|
2-8 °C
|
1 vial
(500 μL)
|
|
|
|
SULFO-TAG Anti-Phospho-STAT3 Antibody
|
2-8 °C
|
1 vial
(375 μL)
|
|
|
|
ERK-STAT3 Cascade Plate
|
2-8 °C
|
5 each
(1 each)
|
|
|
|
|
2-8 °C
|
1 bottle
(50 mL)
|
|
|
|
|
2-8 °C
|
1 bottle
(200 mL)
|
|
|
|
|
RT
|
1 bottle
(50 mL)
|
|
|
|
|
RT
|
1 bottle
(15 g)
|
|
|
|
|
≤-10 °C
|
1 vial
(0.2 mL)
|
|
|
|
Product Inserts
|
|
|
|
Datasheets
|
|
|
|
SDS
|
|
|
|
Title
|
Journal
|
Year
|
|
A randomized, placebo-controlled study of the pharmacokinetics, pharmacodynamics, and tolerability of the oral JAK2 inhibitor fedratinib (SAR302503) in healthy volunteers.
|
|
|
|
Characterization of a high-affinity human antibody with a disulfide bridge in the third complementarity-determining region of the heavy chain.
|
|
|
|
Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233.
|
|
|
|
Development and Utilization of Activated STAT3 Detection Assays for Screening a Library of Secreted Proteins.
|
|
|
|
Cooperative signaling through the signal transducer and activator of transcription 3 and nuclear factor-{kappa}B pathways in subtypes of diffuse large B-cell lymphoma.
|
|
|
|
|
|
Order Details
Catalog No
Size
Price in USD
Qty
Meso Scale Japan 株式会社