Calibrator 22 is a lyophilized blend of BCMA/TNFRSF17, gp130 (soluble), HAVCR2/TIM-3, and Tie-2. It is intended for use as an assay calibrator for MSD U-PLEX human assays that measure any of the included proteins. The utility of this product has not been evaluated in other applications. Individual analyte concentrations are provided in the lot-specific certificates of analysis. BCMA/TNFRSF17 was expressed in a human cell line, HAVCR2/TIM-3 and Tie-2 were expressed in a mouse cell line, and gp130 (soluble) was expressed in an insect cell line. Instructions on using Calibrator 22 are provided in U-PLEX Immuno-Oncology Group 1 Human Product Inserts.