Akt Calibrator is a full-length, recombinant human Akt1 protein expressed in baculovirus and phosphorylated in vitro using phosphoinositide-dependent protein kinase-1 and MAP kinase-activated protein kinase 2. Supplied as a 20X concentrated frozen liquid, it is intended for use as a calibrator for MSD assays that measure phospho-Akt (Ser473) and total Akt.