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Home > References > 2013 > Use of Meso Scale Discovery™ to examine cytokine content in microglia cell supernatant.

Use of Meso Scale Discovery™ to examine cytokine content in microglia cell supernatant.

Burguillos MA.
Journal   Methods Mol Biol.
Species  
Analytes Measured  
Matrix Tested   Cell culture supernatants
Year   2013
Volume   1041
Page Numbers   93-100
Application   Cytokines and Chemokines
Abstract
Cytokine production by activated microglia is one of the hallmarks of inflammatory response in the CNS. The cytokines released by microglia cells can be very different depending on the proinflammatory stimulus.Traditionally, to quantify these different cytokines, the "Sandwich"-enzyme-linked immunosorbent assay (Sandwich-ELISA) has been used. In this chapter we will discuss and describe an improved protocol of the Sandwich-ELISA developed by Meso-Scale Discovery based on an electrochemiluminescence detection system, which allows the ultralow detection of multiple cytokines in microglia cell supernatant.

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