Changes in novel biomarkers of disease activity in juvenile and adult dermatomyositis are sensitive biomarkers of disease course.

Reed AM, Peterson E, Bilgic H, Ytterberg SR, Amin S, Hein MS, Crowson CS, Ernste F, Gillespie EB.
Journal   Arthritis Rheum.
Species  
Analytes Measured   I-TAC , IL-10 , IL-6 , IL-8 , IP-10 , MCP-1 , MIG , TNF-RI
Matrix Tested   Serum
Year   2012
Volume  
Page Numbers  
Application   Cytokines and Chemokines
Abstract
OBJECTIVE: Muscle enzyme levels are insensitive markers of disease activity in juvenile and adult dermatomyositis (DM), especially during the active treatment phase. To improve our ability to monitor DM disease activity longitudinally, especially in the presence of immune modulating agents, we prospectively evaluated whether IFN-dependent peripheral blood gene and chemokine signatures could serve as sensitive and responsive biomarkers for change in disease activity in adult and juvenile DM.

METHODS: Peripheral blood and clinical data were collected from 51 juvenile and adult DM subjects prospectively over 2 study visits. Disease activity measures, whole-blood type I IFN gene and chemokine score were collected. We also measured serum levels of other pro-inflammatory cytokines, including IL-6.

RESULTS: Changes in juvenile and adult DM global disease activity correlated positively and significantly with changes in the type I IFN gene score before (r=0.33, p=0.023) and IFN chemokine score before and after adjustment for medication use (r=0.53, p<0.001 and r=0.50, p=<0.001). Changes in muscle and extramuscular VAS subscales positively correlated with change in IFN gene and chemokine score (p=0.002). Serum levels of IL-6, IL-8 and TNFα were positively correlated with changes in global, muscle and extra-muscular VAS before and after adjustment for medications (p<0.05).

CONCLUSION: Our findings suggest that changes in type I IFN gene and chemokine scores as well as levels of IL-6, IL-8 and TNFα may serve as sensitive and responsive longitudinal biomarkers of change in disease activity in juvenile and adult DM, even in the presence of immunosuppressant use.

View Publications

Related Products

U-PLEX Chemokine Combo 1 (mouse)
IP-10, KC/GRO, MCP-1, MDC, MIP-1α, MIP-1β, MIP-2, MIP-3α | Mouse
Multiplex
NEW
U-PLEX CAR-T Cell Safety Combo 1 (human)
GM-CSF, IFN-γ, IL-1β, IL-6, IL-10, MCP-1, TNF-α | Human
Multiplex
NEW
U-PLEX CAR-T Cell Efficacy Combo 1 (human)
Granzyme B, IFN-γ, IL-2, IL-6, IL-12p70, IP-10, Perforin, TNF-α | Human
Multiplex
NEW
U-PLEX Alzheimer's Disease Combo 1 (human)
Eotaxin, IL-1α, IL-12/IL-23p40, IL-16, IL-17A, MCP-1, MDC, MIF, SDF-1α, YKL-40 | Human
Multiplex
NEW
U-PLEX Adipokine Combo 1 (mouse)
BDNF, IL-1β, IL-6, IL-10, Insulin, Leptin, MCP-1, TNF-α | Mouse
Multiplex
NEW
U-PLEX Metabolic Hormone Combo 1 (mouse)
C-Peptide, Ghrelin (active), GLP-1 (active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PYY (total), TNF-α | Mouse
Multiplex
NEW
U-PLEX Macrophage M2 Combo 1 (mouse)
IL-4, IL-10, IL-13, MDC, TARC | Mouse
Multiplex
NEW
U-PLEX Macrophage M1 Combo 1 (mouse)
IL-1β, IL-6, IL-12p70, IL-15, IL-23, IP-10, MCP-1, MIP-1α, TNF-α | Mouse
Multiplex
NEW
U-PLEX TBI Combo 1 (human)
CD40L (soluble), Fractalkine, IFN-γ, IL-1β, IL-6, IL-18, MCP-1, RANKL/TNFSF11, TNF-α, VEGF-D | Human
Multiplex
NEW
U-PLEX Proinflam Combo 1 (mouse)
IFN-γ, IL-1β, IL-2, IL-6, IL-10, IL-12p70, IL-17A, IP-10, MCP-1, TNF-α | Mouse
Multiplex
NEW
U-PLEX Viral Combo 1 (human)
G-CSF, GM-CSF, IFN-α2a, IFN-β, IFN-γ, IL-1β, IL-1RA, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IP-10, MCP-1, MIP-1α, TNF-α, VEGF-A | Human
Multiplex
NEW
U-PLEX Viral Combo 1 (NHP)
G-CSF, GM-CSF, IFN-α2a, IFN-γ, IL-1β, IL-1RA, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IP-10, MCP-1, MIP-1α, TNF-α, VEGF-A | Non-human primate
Multiplex
NEW
U-PLEX Oncolysis Combo 1 (human)
CTLA-4, IFN-α2a, IFN-β, IL-1β, IL-2Rα, IL-6, IL-8, IP-10, LAG3, MIP-1α, PD1 (epitope 1), TIGIT | Human
Multiplex
NEW
U-PLEX Multiple Sclerosis Combo 1 (human)
CTACK, Fractalkine, GRO-α, IL-7, IL-12/IL-23p40, IP-10, MCP-1, MIP-1β, MIP-3α | Human
Multiplex
NEW
U-PLEX Macrophage M1 Combo 1 (human)
IL-1β, IL-6, IL-12p70, IL-18, IL-23, IP-10, MCP-1, MIP-1α, TNF-α | Human
Multiplex
NEW
U-PLEX Macrophage M2 Combo 1 (human)
Eotaxin-2, IL-4, IL-10, IL-13, M-CSF, MDC, TARC | Human
Multiplex
NEW
S-PLEX Proinflammatory Panel 1 (human) Kit
IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17A, TNF-α | Human
Multiplex
NEW
Human MCP-1 Antibody
MCP-1 | Human
Human IP-10 Antibody
IP-10 | Human
Chemokine Panel 1 (human) Gen. B Control Pack
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Human
V-PLEX Human MCP-1 Gen. B Kit
MCP-1 | Human
Singleplex
V-PLEX Human IP-10 Gen. B Kit
IP-10 | Human
Singleplex
V-PLEX NHP MCP-1 Gen. B Kit
MCP-1 | Non-human primate
Singleplex
V-PLEX NHP IP-10 Gen. B Kit
IP-10 | Non-human primate
Singleplex
V-PLEX Plus Human IP-10 Gen. B Kit
IP-10 | Human
Singleplex
V-PLEX Plus Human MCP-1 Gen. B Kit
MCP-1 | Human
Singleplex
V-PLEX Plus Chemokine Panel 1 (NHP) Gen. B Kit
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Non-human primate
Multiplex
V-PLEX Chemokine Panel 1 (NHP) Gen. B Kit
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Non-human primate
Multiplex
V-PLEX Plus Chemokine Panel 1 (human) Gen. B Kit
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Human
Multiplex
V-PLEX Chemokine Panel 1 (human) Gen. B Kit
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Human
Multiplex
V-PLEX Plus NHP MCP-1 Gen. B Kit
MCP-1 | Non-human primate
Singleplex
V-PLEX Plus NHP IP-10 Gen. B Kit
IP-10 | Non-human primate
Singleplex
R-PLEX Mouse I-TAC Antibody Set
I-TAC | Mouse
R-PLEX Mouse I-TAC Assay
I-TAC | Mouse
Singleplex
U-PLEX Human MIG Antibody Set
MIG | Human
U-PLEX Human TNF-RI Antibody Set
TNF-RI | Human
U-PLEX Human TNF-RI Assay
TNF-RI | Human
Singleplex
U-PLEX Human TNF-RI 384-well Assay
TNF-RI | Human
Singleplex
U-PLEX Human MIG Assay
MIG | Human
Singleplex
U-PLEX Human MIG 384-well Assay
MIG | Human
Singleplex
U-PLEX Mouse IL-10 384-Well Assay
IL-10 | Mouse
Singleplex
U-PLEX Mouse MCP-1 384-Well Assay
MCP-1 | Mouse
Singleplex
U-PLEX Mouse IP-10 384-Well Assay
IP-10 | Mouse
Singleplex
U-PLEX Mouse IL-6 384-Well Assay
IL-6 | Mouse
Singleplex
U-PLEX Mouse TNF-RI 384-Well Assay
TNF-RI | Mouse
Singleplex
U-PLEX NHP IL-10 384-Well Assay
IL-10 | Non-human primate
Singleplex
U-PLEX NHP IP-10 384-Well Assay
IP-10 | Non-human primate
Singleplex
U-PLEX NHP IL-8 384-Well Assay
IL-8 | Non-human primate
Singleplex
U-PLEX NHP IL-6 384-Well Assay
IL-6 | Non-human primate
Singleplex
U-PLEX NHP MCP-1 384-Well Assay
MCP-1 | Non-human primate
Singleplex
U-PLEX NHP I-TAC 384-Well Assay
I-TAC | Non-human primate
Singleplex
U-PLEX Human IL-10 384-Well Assay
IL-10 | Human
Singleplex
U-PLEX Human IL-8 384-Well Assay
IL-8 | Human
Singleplex
U-PLEX Human IL-6 384-Well Assay
IL-6 | Human
Singleplex
U-PLEX Human MCP-1 384-Well Assay
MCP-1 | Human
Singleplex
U-PLEX Human I-TAC 384-Well Assay
I-TAC | Human
Singleplex
U-PLEX Human IP-10 384-Well Assay
IP-10 | Human
Singleplex
S-PLEX NHP IL-6 Kit
IL-6 | Non-human primate
Singleplex
S-PLEX Human IL-6 Kit
IL-6 | Human
Singleplex
S-PLEX Human IL-10 Kit
IL-10 | Human
Singleplex
V-PLEX Plus Viral Panel 3 Human Kit
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α | Human
Multiplex
V-PLEX Plus Viral Panel 3 NHP Kit
IFN-γ, IL-1β, IL-5, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Viral Panel 3 NHP Kit
IFN-γ, IL-1β, IL-5, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Viral Panel 3 Human Kit
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α | Human
Multiplex
V-PLEX Plus Viral Panel 2 Human Kit
IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-10, TNF-α | Human
Multiplex
V-PLEX Plus Viral Panel 1 NHP Kit
IL-1β, IL-6, IL-8 | Non-human primate
Multiplex
V-PLEX Plus Viral Panel 1 Human Kit
IL-1β, IL-6, IL-8, TNF-α | Human
Multiplex
V-PLEX Viral Panel 2 NHP Kit
IFN-γ, IL-1β, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Viral Panel 2 Human Kit
IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-10, TNF-α | Human
Multiplex
V-PLEX Viral Panel 1 NHP Kit
IL-1β, IL-6, IL-8 | Non-human primate
Multiplex
V-PLEX Viral Panel 1 Human Kit
IL-1β, IL-6, IL-8, TNF-α | Human
Multiplex
V-PLEX Plus Viral Panel 2 NHP Kit
IFN-γ, IL-1β, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
U-PLEX Mouse TNF-RI Assay
TNF-RI | Mouse
Singleplex
R-PLEX Mouse MIG Assay
MIG | Mouse
Singleplex
R-PLEX Rat MCP-1 Assay
MCP-1 | Rat
Singleplex
R-PLEX Rat IL-6 Assay
IL-6 | Rat
Singleplex
R-PLEX Rat IL-10 Assay
IL-10 | Rat
Singleplex
U-PLEX Mouse TNF-RI Antibody Set
TNF-RI | Mouse
R-PLEX Mouse MIG Antibody Set
MIG | Mouse
R-PLEX Rat MCP-1 Antibody Set
MCP-1 | Rat
R-PLEX Rat IL-10 Antibody Set
IL-10 | Rat
R-PLEX Rat IL-6 Antibody Set
IL-6 | Rat
Cytokine Panel 1 Mouse Control Pack
IL-9, IL-15, IL-17A/F, IL-27p28/IL-30, IL-33, IP-10, MCP-1, MIP-1α, MIP-2 | Mouse
U-PLEX Human MCP-1 Antibody Set
MCP-1 | Human
U-PLEX Human I-TAC Antibody Set
I-TAC | Human
U-PLEX Human IP-10 Antibody Set
IP-10 | Human
U-PLEX Human IL-10 Antibody Set
IL-10 | Human
U-PLEX Human IL-8 Antibody Set
IL-8 | Human
U-PLEX Human IL-6 Antibody Set
IL-6 | Human
Rat IL-6 Antibody
IL-6
Mouse IL-10 Antibody
IL-10
Proinflammatory Panel 1 Rat Control Pack
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-13, KC/GRO, TNF-α | Rat
Proinflammatory Panel 1 Mouse Control Pack
IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, KC/GRO, TNF-α | Mouse
Chemokine Panel 1 Human Control Pack
Eotaxin, Eotaxin-3, IL-8, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Human
Human IL-8 Antibody Bulk Product
IL-8
Human IL-6 Antibody Bulk Product
IL-6
Proinflammatory Panel 1 Human Control Pack
IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-α | Human
Rat IL-10 Antibody
IL-10
Mouse MCP-1 Antibody
MCP-1 | Mouse
Mouse IP-10 Antibody
IP-10 | Mouse
Product Search
Browse Our Products

By Analytes
By Applications
Search
Meso Scale Japan 株式会社