spot difference
  • Sign In
  • Register
  • Cart (0)
Home > References > 2013 > Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice.

Beneficial effects of parenteral GLP-1 delivery by cell therapy in insulin-deficient streptozotocin diabetic mice.

Vasu S, Moffett RC, McCluskey JT, Hamid MH, Irwin N, Flatt PR.
Journal   Gene Ther.
Species  
Analytes Measured   GLP-1 , Glucagon
Matrix Tested   Plasma
Year   2013
Volume  
Page Numbers  
Application   Metabolic
Abstract
Parenteral delivery of long-acting glucagon-like peptide-1 (GLP-1) mimetics has received much attention as a therapeutic option for diabetes. However, cell therapy-based GLP-1 treatments may provide a more physiological regulation of blood glucose. The present study assessed the effects of chronic GLP-1 delivery by cell therapy, using the GLP-1-secreting GLUTag cell line, in normoglycemic and streptozotocin-induced diabetic mice. GLUTag cell aggregates were transplanted into the subscapular region of mice. Over 30 days, cellular transplantation gave rise to encapsulated and well-vascularized growths, which contained immunoreactive GLP-1. Cell implantation was well tolerated and had no appreciable metabolic effects in normal mice. However, transplantation significantly (P<0.001) countered excessive food and fluid intake in diabetic mice and maintained normal body weight. Circulating glucose (P<0.01) and glucagon (P<0.05) were significantly reduced and plasma insulin and GLP-1 dramatically increased. This was associated with significantly (P<0.01) improved glucose tolerance in diabetic mice. Histological examination of the pancreata of these mice revealed elevations (P<0.001) in islet and β-cell area, with reduced (P<0.001) α-cell area. Increased β-cell mass reflected the enhanced proliferation relative to apoptosis. These studies emphasize the potential of chronic GLP-1 delivery by cell therapy as a potential therapeutic option for diabetes.

View Publications
View Publications

Related Products

U-PLEX Diabetes Combo 1 (mouse)
C-Peptide, GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
U-PLEX Metabolic 3-Plex Combo 1 (mouse)
GLP-1 (total), Glucagon, Insulin | Mouse
Multiplex
U-PLEX Microbiome Combo 1 (human)
Ghrelin (total), GLP-1 (total), Glucagon, Insulin, Leptin, PP, PYY (total) | Human
Multiplex
U-PLEX Diabetes Combo 1 (rat)
C-Peptide, GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
U-PLEX Metabolic 3-Plex Combo 1 (rat)
GLP-1 (total), Glucagon, Insulin | Rat
Multiplex
U-PLEX Diabetes Combo 1 (human)
C-Peptide, GIP (total), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Human
Multiplex
U-PLEX Custom Metabolic Group 1 (rat) 384-well Assays
Rat
Multiplex
U-PLEX Mouse GLP-1 (total) 384-Well Assay
GLP-1 (total) | Mouse
Singleplex
U-PLEX Rat GLP-1 (total) 384-Well Assay
GLP-1 (total) | Rat
Singleplex
U-PLEX Custom Metabolic Group 1 (mouse) 384-well Assays
BAFF, BCA-1/BLC, BDNF, CD40/TNFRSF5, C-Peptide, Eotaxin, EPO, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, IFN-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27p28/IL-30, IL-31, IL-33, Insulin, IP-10, KC/GRO, Leptin, MCP-1, MCP-5/CCL12, MDC, MIP-1α, MIP-1β, MIP-2, MIP-3α, MMP-9 (total), PYY (total), RANTES, TARC, TNF-α, VEGF-A | Mouse
Multiplex
U-PLEX Mouse Glucagon 384-Well Assay
Glucagon | Mouse
Singleplex
U-PLEX Rat Glucagon 384-Well Assay
Glucagon | Rat
Singleplex
U-PLEX Human Glucagon 384-well Assay
Glucagon | Human
Singleplex
U-PLEX Human GLP-1 (total) 384-well Assay
GLP-1 (total) | Human
Singleplex
U-PLEX Custom Metabolic Group 1 (human) 384-well Assays
BAFF, BDNF, β-NGF, C-Peptide, CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FGF-21, FGF-23, FLT3L, Fractalkine, FSH, G-CSF, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive), GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, Insulin, IP-10, Leptin, Luteinizing Hormone (LH), MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-5, PP, Proinsulin, PYY (total), SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, YKL-40 | Human
Multiplex
Glucagon Antibody
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
V-PLEX Metabolic Panel 1 Rat Kit
GIP (active), GLP-1 (active), Glucagon | Rat
Multiplex
V-PLEX Metabolic Panel 1 NHP Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, PP | Non-human primate
Multiplex
V-PLEX Metabolic Panel 1 Mouse Kit
GLP-1 (active), Glucagon | Mouse
Multiplex
V-PLEX Metabolic Panel 1 Human Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, Leptin, PP | Human
Multiplex
V-PLEX Metabolic Panel 1 Canine Kit
GIP (active), GLP-1 (active), Glucagon, Insulin | Canine
Multiplex
V-PLEX Plus Metabolic Panel 1 Rat Kit
GIP (active), GLP-1 (active), Glucagon | Rat
Multiplex
V-PLEX Plus Metabolic Panel 1 NHP Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, PP | Non-human primate
Multiplex
V-PLEX Plus Metabolic Panel 1 Mouse Kit
GLP-1 (active), Glucagon | Mouse
Multiplex
V-PLEX Plus Metabolic Panel 1 Human Kit
C-Peptide, GIP (active), GLP-1 (active), Glucagon, Insulin, Leptin, PP | Human
Multiplex
V-PLEX Plus Metabolic Panel 1 Canine Kit
GIP (active), GLP-1 (active), Glucagon, Insulin | Canine
Multiplex
V-PLEX Plus Glucagon Kit
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
Singleplex
V-PLEX Glucagon Kit
Glucagon | Human, Mouse, Rat, Non-human primate, Canine
Singleplex
U-PLEX Rat Glucagon Assay
Glucagon | Rat
Singleplex
U-PLEX Mouse GLP-1 (total) Assay
GLP-1 (total) | Mouse
Singleplex
U-PLEX Mouse Glucagon Assay
Glucagon | Mouse
Singleplex
U-PLEX Rat GLP-1 (total) Assay
GLP-1 (total) | Rat
Singleplex
U-PLEX Human Glucagon Assay
Glucagon | Human
Singleplex
U-PLEX Human GLP-1 (total) Assay
GLP-1 (total) | Human
Singleplex
U-PLEX Custom Metabolic Group 1 (rat) Assays
Rat
Multiplex
U-PLEX Custom Metabolic Group 1 (mouse) Assays
Mouse
Multiplex
U-PLEX Obesity Combo 2 (rat)
C-Peptide, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) | Rat
Multiplex
U-PLEX Metabolic 3-Plex Combo 2 (rat)
GLP-1 (active), Glucagon, Insulin | Rat
Multiplex
U-PLEX Metabolic 3-Plex Combo 2 (mouse)
GLP-1 (active), Glucagon, Insulin | Mouse
Multiplex
U-PLEX Metabolic 2-Plex Combo 2 (rat)
Glucagon, Insulin | Rat
Multiplex
U-PLEX Metabolic 2-Plex Combo 2 (mouse)
Glucagon, Insulin | Mouse
Multiplex
U-PLEX Gut Hormone Combo 1 (mouse)
Ghrelin (active), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
U-PLEX Obesity Combo 2 (mouse)
C-Peptide, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) | Mouse
Multiplex
U-PLEX Obesity Combo 1 (rat)
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin | Rat
Multiplex
U-PLEX Obesity Combo 1 (mouse)
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin | Mouse
Multiplex
U-PLEX Metabolic Hormones Combo 1 (rat)
C-Peptide, Ghrelin (active), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
U-PLEX Metabolic Combo 1 (rat)
BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Rat
Multiplex
U-PLEX Metabolic Combo 1 (mouse)
BAFF, BDNF, C-Peptide, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, PYY (total) | Mouse
Multiplex
U-PLEX Metabolic Hormones Combo 1 (mouse)
C-Peptide, Ghrelin (active), GLP-1 (active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PYY (total), TNF-α | Mouse
Multiplex
U-PLEX Metabolic 4-Plex Combo 1 (human)
GLP-1 (active), Glucagon, Insulin, Leptin | Human
Multiplex
U-PLEX Metabolic 3-Plex Combo 1 (human)
GLP-1 (active), Glucagon, Insulin | Human
Multiplex
U-PLEX Custom Metabolic Group 1 (human) Assays
BAFF, BDNF, β-NGF, C-Peptide, CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FGF-21, FGF-23, FLT3L, Fractalkine, FSH, G-CSF, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive), GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, Insulin, IP-10, Leptin, Luteinizing Hormone (LH), MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-5, PP, Proinsulin, PYY (total), SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, YKL-40 | Human
Multiplex
U-PLEX Glucagon Antibody Set (5 Plate Size)
Glucagon | Human, Mouse, Rat
U-PLEX GLP-1 (total) Antibody Set (5 Plate Size)
GLP-1 (total) | Human, Mouse, Rat
U-PLEX Obesity Combo 1 (human)
BDNF, FGF-21, Ghrelin (total), Glucagon, Leptin | Human
Multiplex
U-PLEX Metabolic Combo 1 (human)
BAFF, BDNF, β-NGF, C-Peptide, FGF-21, FGF-23, FSH, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive), GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, Insulin, Leptin, Luteinizing Hormone (LH), PP, Proinsulin, PYY (total) | Human
Multiplex
U-PLEX Diabetes Combo 2 (human)
C-Peptide, GIP (total), GLP-1 (active), Glucagon, Insulin, Leptin, PYY (total) | Human
Multiplex
U-PLEX Obesity Combo 2 (human)
C-Peptide, FGF-23, Ghrelin (total), GLP-1 (total), Insulin, Leptin, PYY (total) | Human
Multiplex
GLP-1 (total) Control Pack
GLP-1 (total) | Human, Mouse, Non-human primate, Rat, Canine
V-PLEX Plus GLP-1 (total) Kit
GLP-1 (total) | Human, Mouse, Non-human primate, Rat, Canine
Singleplex
V-PLEX GLP-1 (total) Kit
GLP-1 (total) | Human, Mouse, Non-human primate, Rat, Canine
Singleplex
GLP-1 (total) Antibody
GLP-1 (total) | Human
Browse Our Products

By Analytes
By Applications
Search
Customer Service/Orders


Scientific/Technical Support


Instrument Support


Company Headquarters