Renal biomarker changes associated with hyaline droplet nephropathy in rats are time and potentially compound dependent.

Register
Cart (0)

Home > References > 2012 > Renal biomarker changes associated with hyaline droplet nephropathy in rats are time and potentially compound dependent.

Contact an Account Manager

Renal biomarker changes associated with hyaline droplet nephropathy in rats are time and potentially compound dependent.

Brott DA, Bentley P, Nadella MV, Thurman D, Fikes J, Cheatham L, McGrath F, Luo W, Kinter LB.

Journal		Toxicology
Species
Analytes Measured		Albumin , Clusterin , HAVCR1 KIM-1 , Osteopontin , RPA-1
Matrix Tested		Urine

Year		2012
Volume
Page Numbers
Application		Kidney Injury , Toxicology

Abstract

Alpha 2µ-globulin mediated hyaline droplet nephropathy (HDN) is a male rat specific lesion induced when a compound or metabolite binds to alpha 2µ-globulin. The objective of this study was to investigate if the newer and more sensitive renal biomarkers would be altered with HDN as well as be able to distinguish between HDN and oxidative stress-induced kidney injury. Rats were dosed orally for 7 days to determine (1) if HDN (induced by 2-propanol or d-limonene) altered the newer renal biomarkers and not BUN or creatinine, (2) if renal biomarkers could distinguish between HDN and oxidative stress-induced kidney injury (potassium bromate), (3) sensitivity of HDN-induced renal biomarker changes relative to d-limonene dose, and (4) reversibility of HDN and renal biomarkers, using vehicle or 300mg/kg/day d-limonene with 7 days of dosing and necropsies scheduled over the period of Days 8-85. HDN-induced renal biomarker changes in male rats were potentially compound specific: (1) 2-propanol induced mild HDN without increased renal biomarkers, (2) potassium bromate induced moderate HDN with increased clusterin, and (3) d-limonene induced marked HDN with increased αGST, µGST and albumin. Administration of potassium bromate did not result in oxidative stress-induced kidney injury, based on histopathology and renal biomarkers creatinine and BUN. The compound d-limonene induced a dose dependent increase in HDN severity and renal biomarker changes without altering BUN, creatinine or NAG: (1) minimal induction of HDN and no altered biomarkers at 10mg/kg/day, (2) mild induction of HDN with increased αGST and µGST at 50mg/kg/day and (3) marked induction of HDN with increased αGST, µGST and albumin at 300mg/kg/day. HDN induced by d-limonene was reversible, but with a variable renal biomarker pattern over time: Day 8 there was increased αGST, µGST and albumin; on Day 15 increased clusterin, albumin and Kim-1. In summary, HDN altered the newer and more sensitive renal biomarkers in a time and possibly compound dependent manner.

View Publications

Related Products


U-PLEX Human HAVCR1/KIM-1 Antibody Set HAVCR1/KIM-1 \| Human
U-PLEX Human HAVCR1/KIM-1 Assay, 384-well HAVCR1/KIM-1 \| Human Singleplex
U-PLEX Human HAVCR1/KIM-1 Assay HAVCR1/KIM-1 \| Human Singleplex
R-PLEX Mouse HAVCR1/KIM-1 Antibody Set HAVCR1/KIM-1 \| Mouse
R-PLEX Mouse HAVCR1/KIM-1 Assay HAVCR1/KIM-1 \| Mouse Singleplex
U-PLEX Human Clusterin 384-Well Assay Clusterin \| Human Singleplex
U-PLEX Custom Biomarker Group 3 (human) 384-well Assays Human Multiplex
U-PLEX Custom Biomarker Group 3 (human) Assays Human Multiplex
U-PLEX Human Clusterin Antibody Set Clusterin \| Human
U-PLEX Metabolic Combo 2 (human) Adiponectin, ApoA1, ApoC3, Clusterin, CRP, DPPIV, NGAL/LCN2, RBP4, SHBG, sTfR-1 \| Human Multiplex
U-PLEX Kidney Injury Combo 1 (human) A2M, Clusterin, Cystatin C, NGAL/LCN2, RBP4, Serpin A1 \| Human Multiplex
U-PLEX Human Clusterin Assay Clusterin \| Human Singleplex
R-PLEX Rat Albumin Antibody Set Albumin \| Rat
R-PLEX Human Albumin Assay Albumin \| Human Singleplex
R-PLEX Rat Osteopontin Assay Osteopontin \| Rat Singleplex
R-PLEX Rat HAVCR1/KIM-1 Assay HAVCR1/KIM-1 \| Rat Singleplex
R-PLEX Human HAVCR1/KIM-1 Assay HAVCR1/KIM-1 \| Human Singleplex
R-PLEX Rat Albumin Assay Albumin \| Rat Singleplex
R-PLEX Mouse Clusterin Assay Clusterin \| Mouse Singleplex
R-PLEX Human Osteopontin Assay Osteopontin \| Human Singleplex
R-PLEX Mouse Clusterin Antibody Set Clusterin \| Mouse
R-PLEX Human Albumin Antibody Set Albumin \| Human
R-PLEX Human HAVCR1/KIM-1 Antibody Set HAVCR1/KIM-1 \| Human
R-PLEX Rat Osteopontin Antibody Set Osteopontin \| Rat
R-PLEX Rat HAVCR1/KIM-1 Antibody Set HAVCR1/KIM-1 \| Rat
R-PLEX Human Osteopontin Antibody Set Osteopontin \| Human
Kidney Injury Panel 3 Human Kit Calbindin, Clusterin, HAVCR1/KIM-1, Osteoactivin, TFF3, VEGF-A \| Human Multiplex
Rat Clusterin Kit Clusterin \| Rat Singleplex
Kidney Injury Panel 1 Rat Kit Albumin, HAVCR1/KIM-1, NGAL/LCN2, Osteopontin \| Rat Multiplex
U-PLEX Immuno-Oncology Group 1 (human) 384-well Assays BAFF-R/TNFRSF13C, BCMA/TNFRSF17, CD20, CD27, CD28, CD40L (soluble), CD276/B7-H3, CTACK, CTLA-4, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FGF (basic), FLT3L, Fractalkine, G-CSF, GITR/TNFRSF18, GITRL/TNFSF18, GM-CSF, gp130 (soluble), Granzyme A, Granzyme B, GRO-α, HAVCR2/TIM-3, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, IP-10, I-TAC, LAG3, MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-1β, MIP-5, OX40/TNFRSF4, PD1 (epitope 1), PD1 (epitope 2), PD-L1 (epitope 1), PD-L2, PlGF, RANKL/TNFSF11, Tie-2, TIGIT, TLR1, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, VEGF-D, YKL-40 \| Human Multiplex
U-PLEX Immuno-Oncology Group 1 (human) Assays APRIL/TNFSF13, BAFF-R/TNFRSF13C, BCMA/TNFRSF17, CD20, CD27, CD28, CD40L (soluble), CD276/B7-H3, CTACK, CTLA-4, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, E-Selectin, FGF (basic), FLT3L, Fractalkine, Galectin-9, G-CSF, GITR/TNFRSF18, GITRL/TNFSF18, GM-CSF, gp130 (soluble), Granzyme A, Granzyme B, GRO-α, HAVCR2/TIM-3, HVEM/TNFRSF14, I-309, ICOS, ICOS-L/B7-H2, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, IP-10, I-TAC, LAG3, LIGHT/TNFSF14, MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIG, MIP-1α, MIP-1β, MIP-5, MMP-1, MMP-2, MMP-7, MMP-9 (total), Nectin-4, OX40/TNFRSF4, PD1 (epitope 1), PD1 (epitope 2), PD-L1 (epitope 1), PD-L1 (epitope 2), PD-L2, Pentraxin 3, Perforin, PlGF, proMMP-9, P-Selectin, RAGE (soluble), RANKL/TNFSF11, RANTES, S100A12, Tie-2, TIGIT, TLR1, TNF-α, TNF-β, TNF-RI, TNF-RII, TPO, TRAIL, TSLP, VEGF-A, VEGF-D, VEGFR-1/Flt-1, YKL-40 \| Human Multiplex
U-PLEX Immuno-Oncology Group 1 (human) Assays APRIL/TNFSF13, BAFF-R/TNFRSF13C, BCMA/TNFRSF17, CD20, CD27, CD28, CD40L (soluble), CD276/B7-H3, CTACK, CTLA-4, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, E-Selectin, FGF (basic), FLT3L, Fractalkine, Galectin-9, G-CSF, GITR/TNFRSF18, GITRL/TNFSF18, GM-CSF, gp130 (soluble), Granzyme A, Granzyme B, GRO-α, HAVCR2/TIM-3, HVEM/TNFRSF14, I-309, ICOS, ICOS-L/B7-H2, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, IP-10, I-TAC, LAG3, LIGHT/TNFSF14, MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIG, MIP-1α, MIP-1β, MIP-5, MMP-1, MMP-2, MMP-7, MMP-9 (total), Nectin-4, OX40/TNFRSF4, PD1 (epitope 1), PD1 (epitope 2), PD-L1 (epitope 1), PD-L1 (epitope 2), PD-L2, Pentraxin 3, Perforin, PlGF, proMMP-9, P-Selectin, RAGE (soluble), RANKL/TNFSF11, RANTES, S100A12, Tie-2, TIGIT, TLR1, TNF-α, TNF-β, TNF-RI, TNF-RII, TPO, TRAIL, TSLP, VEGF-A, VEGF-D, VEGFR-1/Flt-1, YKL-40 \| Human Multiplex

R-PLEX: Matched Antibody Sets - Suitable for Many Research Applications

Quick Links

Browse Our Products

By Analytes

By Applications

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA


U-PLEX Human HAVCR1/KIM-1 Antibody Set HAVCR1/KIM-1 \| Human
U-PLEX Human HAVCR1/KIM-1 Assay, 384-well HAVCR1/KIM-1 \| Human Singleplex
U-PLEX Human HAVCR1/KIM-1 Assay HAVCR1/KIM-1 \| Human Singleplex
R-PLEX Mouse HAVCR1/KIM-1 Antibody Set HAVCR1/KIM-1 \| Mouse
R-PLEX Mouse HAVCR1/KIM-1 Assay HAVCR1/KIM-1 \| Mouse Singleplex
U-PLEX Human Clusterin 384-Well Assay Clusterin \| Human Singleplex
U-PLEX Custom Biomarker Group 3 (human) 384-well Assays Human Multiplex
U-PLEX Custom Biomarker Group 3 (human) Assays Human Multiplex
U-PLEX Human Clusterin Antibody Set Clusterin \| Human
U-PLEX Metabolic Combo 2 (human) Adiponectin, ApoA1, ApoC3, Clusterin, CRP, DPPIV, NGAL/LCN2, RBP4, SHBG, sTfR-1 \| Human Multiplex
U-PLEX Kidney Injury Combo 1 (human) A2M, Clusterin, Cystatin C, NGAL/LCN2, RBP4, Serpin A1 \| Human Multiplex
U-PLEX Human Clusterin Assay Clusterin \| Human Singleplex
R-PLEX Rat Albumin Antibody Set Albumin \| Rat
R-PLEX Human Albumin Assay Albumin \| Human Singleplex
R-PLEX Rat Osteopontin Assay Osteopontin \| Rat Singleplex
R-PLEX Rat HAVCR1/KIM-1 Assay HAVCR1/KIM-1 \| Rat Singleplex
R-PLEX Human HAVCR1/KIM-1 Assay HAVCR1/KIM-1 \| Human Singleplex
R-PLEX Rat Albumin Assay Albumin \| Rat Singleplex
R-PLEX Mouse Clusterin Assay Clusterin \| Mouse Singleplex
R-PLEX Human Osteopontin Assay Osteopontin \| Human Singleplex
R-PLEX Mouse Clusterin Antibody Set Clusterin \| Mouse
R-PLEX Human Albumin Antibody Set Albumin \| Human
R-PLEX Human HAVCR1/KIM-1 Antibody Set HAVCR1/KIM-1 \| Human
R-PLEX Rat Osteopontin Antibody Set Osteopontin \| Rat
R-PLEX Rat HAVCR1/KIM-1 Antibody Set HAVCR1/KIM-1 \| Rat
R-PLEX Human Osteopontin Antibody Set Osteopontin \| Human
Kidney Injury Panel 3 Human Kit Calbindin, Clusterin, HAVCR1/KIM-1, Osteoactivin, TFF3, VEGF-A \| Human Multiplex
Rat Clusterin Kit Clusterin \| Rat Singleplex
Kidney Injury Panel 1 Rat Kit Albumin, HAVCR1/KIM-1, NGAL/LCN2, Osteopontin \| Rat Multiplex
U-PLEX Immuno-Oncology Group 1 (human) 384-well Assays BAFF-R/TNFRSF13C, BCMA/TNFRSF17, CD20, CD27, CD28, CD40L (soluble), CD276/B7-H3, CTACK, CTLA-4, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FGF (basic), FLT3L, Fractalkine, G-CSF, GITR/TNFRSF18, GITRL/TNFSF18, GM-CSF, gp130 (soluble), Granzyme A, Granzyme B, GRO-α, HAVCR2/TIM-3, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, IP-10, I-TAC, LAG3, MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-1β, MIP-5, OX40/TNFRSF4, PD1 (epitope 1), PD1 (epitope 2), PD-L1 (epitope 1), PD-L2, PlGF, RANKL/TNFSF11, Tie-2, TIGIT, TLR1, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, VEGF-D, YKL-40 \| Human Multiplex
U-PLEX Immuno-Oncology Group 1 (human) Assays APRIL/TNFSF13, BAFF-R/TNFRSF13C, BCMA/TNFRSF17, CD20, CD27, CD28, CD40L (soluble), CD276/B7-H3, CTACK, CTLA-4, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, E-Selectin, FGF (basic), FLT3L, Fractalkine, Galectin-9, G-CSF, GITR/TNFRSF18, GITRL/TNFSF18, GM-CSF, gp130 (soluble), Granzyme A, Granzyme B, GRO-α, HAVCR2/TIM-3, HVEM/TNFRSF14, I-309, ICOS, ICOS-L/B7-H2, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, IP-10, I-TAC, LAG3, LIGHT/TNFSF14, MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIG, MIP-1α, MIP-1β, MIP-5, MMP-1, MMP-2, MMP-7, MMP-9 (total), Nectin-4, OX40/TNFRSF4, PD1 (epitope 1), PD1 (epitope 2), PD-L1 (epitope 1), PD-L1 (epitope 2), PD-L2, Pentraxin 3, Perforin, PlGF, proMMP-9, P-Selectin, RAGE (soluble), RANKL/TNFSF11, RANTES, S100A12, Tie-2, TIGIT, TLR1, TNF-α, TNF-β, TNF-RI, TNF-RII, TPO, TRAIL, TSLP, VEGF-A, VEGF-D, VEGFR-1/Flt-1, YKL-40 \| Human Multiplex
U-PLEX Immuno-Oncology Group 1 (human) Assays APRIL/TNFSF13, BAFF-R/TNFRSF13C, BCMA/TNFRSF17, CD20, CD27, CD28, CD40L (soluble), CD276/B7-H3, CTACK, CTLA-4, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, E-Selectin, FGF (basic), FLT3L, Fractalkine, Galectin-9, G-CSF, GITR/TNFRSF18, GITRL/TNFSF18, GM-CSF, gp130 (soluble), Granzyme A, Granzyme B, GRO-α, HAVCR2/TIM-3, HVEM/TNFRSF14, I-309, ICOS, ICOS-L/B7-H2, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, IP-10, I-TAC, LAG3, LIGHT/TNFSF14, MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIG, MIP-1α, MIP-1β, MIP-5, MMP-1, MMP-2, MMP-7, MMP-9 (total), Nectin-4, OX40/TNFRSF4, PD1 (epitope 1), PD1 (epitope 2), PD-L1 (epitope 1), PD-L1 (epitope 2), PD-L2, Pentraxin 3, Perforin, PlGF, proMMP-9, P-Selectin, RAGE (soluble), RANKL/TNFSF11, RANTES, S100A12, Tie-2, TIGIT, TLR1, TNF-α, TNF-β, TNF-RI, TNF-RII, TPO, TRAIL, TSLP, VEGF-A, VEGF-D, VEGFR-1/Flt-1, YKL-40 \| Human Multiplex

Please Sign In

User name

Password

Renal biomarker changes associated with hyaline droplet nephropathy in rats are time and potentially compound dependent.

Abstract

Related Products

Quick Links

Browse Our Products

Contact Information

Products

Services

Orders & Support

Key Topics